All Outputs (14)

Quorum-sensing, intra- and inter-species competition in the staphylococci (2023)
Journal Article

In Gram-positive bacteria such as Staphylococcus aureus and the coagulase-negative staphylococci (CoNS), the accessory gene regulator (agr) is a highly conserved but polymorphic quorum-sensing system involved in colonization, virulence and biofilm de... Read More about Quorum-sensing, intra- and inter-species competition in the staphylococci.

Conformational analysis and interaction of the Staphylococcus aureus transmembrane peptidase AgrB with its AgrD propeptide substrate (2023)
Journal Article

Virulence gene expression in the human pathogen, S. aureus is regulated by the agr (accessory gene regulator) quorum sensing (QS) system which is conserved in diverse Gram-positive bacteria. The agr QS signal molecule is an autoinducing peptide (AIP)... Read More about Conformational analysis and interaction of the Staphylococcus aureus transmembrane peptidase AgrB with its AgrD propeptide substrate.

The Role of Lipid Chains as Determinants of Membrane Stability in the Presence of Styrene (2022)
Journal Article

Biofermentative production of styrene from renewable carbon sources is crucially dependent on strain tolerance and viability at elevated styrene concentrations. Solvent-driven collapse of bacterial plasma membranes limits yields and is technologicall... Read More about The Role of Lipid Chains as Determinants of Membrane Stability in the Presence of Styrene.

Solid state NMR of membrane proteins: Methods and applications (2021)
Journal Article

Membranes of cells are active barriers, in which membrane proteins perform essential remodelling, transport and recognition functions that are vital to cells. Membrane proteins are key regulatory components of cells and represent essential targets fo... Read More about Solid state NMR of membrane proteins: Methods and applications.

Membrane protein structure determination and characterisation by solution and solid-state nmr (2020)
Journal Article

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Biological membranes define the interface of life and its basic unit, the cell. Membrane proteins play key roles in membrane functions, yet their structure and mechanisms remain poorly underst... Read More about Membrane protein structure determination and characterisation by solution and solid-state nmr.

Immunostimulatory efficacy and protective potential of putative TgERK7 protein in mice experimentally infected by Toxoplasma gondii (2020)
Journal Article

© 2020 The Author(s) The extracellular signal-regulated kinases (ERKs) serve as important determinants of cellular signal transduction pathways, and hence may play important roles during infections. Previous work suggested that putative ERK7 of Toxop... Read More about Immunostimulatory efficacy and protective potential of putative TgERK7 protein in mice experimentally infected by Toxoplasma gondii.

Membrane Stability in the Presence of Methacrylate Esters (2020)
Journal Article

Bioproduction of poly(methyl methacrylate) is a fast growing global industry that is limited by cellular toxicity of monomeric methacrylate intermediates to the producer strains. Maintaining high methacrylate concentrations during biofermentation, re... Read More about Membrane Stability in the Presence of Methacrylate Esters.

Timing is everything – impact of naturally occurring Staphylococcus aureus AgrC cytoplasmic domain adaptive mutations on auto-induction (2019)
Journal Article

Mutations in the polymorphic Staphylococcus aureus agr locus responsible for quorum sensing (QS) dependent virulence gene regulation occur frequently during host adaptation. In two genomically closely related S. aureus clinical isolates exhibiting ma... Read More about Timing is everything – impact of naturally occurring Staphylococcus aureus AgrC cytoplasmic domain adaptive mutations on auto-induction.

Bacterial Resistance to Antibiotics – From Molecules to Man (2019)
Book

© 2020 John Wiley & Sons Ltd. AN AUTHORITATIVE SURVEY OF CURRENT RESEARCH INTO CLINICALLY USEFUL CONVENTIONAL AND NONCONVENTIONAL ANTIBIOTIC THERAPEUTICS Pharmaceutically-active antibiotics revolutionized the treatment of infectious diseases, leadi... Read More about Bacterial Resistance to Antibiotics – From Molecules to Man.

Molecular recognition of lipopolysaccaride by the lantibiotic nisin (2018)
Journal Article
Lanne, A. B., Goode, A., Prattley, C., Kumari, D., Drasbek, M. R., Williams, P., …Bonev, B. B. (2019). Molecular recognition of lipopolysaccaride by the lantibiotic nisin. BBA - Biomembranes, 1861(1), 83-92. https://doi.org/10.1016/j.bbamem.2018.10.006

Nisin is a lanthionine antimicrobial effective against diverse Gram-positive bacteria and is used as a food preservative worldwide. Its action is mediated by pyrophosphate recognition of the bacterial cell wall receptors lipid II and undecaprenyl pyr... Read More about Molecular recognition of lipopolysaccaride by the lantibiotic nisin.

To boil an egg: substrate binding affects critical stability in thermal unfolding of proteins (2018)
Journal Article
Hussain, R., Hughes, C. S., Jávorfi, T., Siligardi, G., Williams, P., & Bonev, B. B. (2018). To boil an egg: substrate binding affects critical stability in thermal unfolding of proteins. Journal of Physical Chemistry B, 122(8), 2213-2218. https://doi.org/10.1021/acs.jpcb.7b10643

Thermal unfolding of proteins is used extensively in screening of drug candidates because molecular interactions with ligands and substrates affect strongly protein stability, transition temperature, and cooperativity. We use synchrotron radiation ci... Read More about To boil an egg: substrate binding affects critical stability in thermal unfolding of proteins.

Docking and molecular dynamics simulations of the ternary complex nisin2:lipid II (2016)
Journal Article
Mulholland, S., Turpin, E. R., Bonev, B. B., & Hirst, J. (2016). Docking and molecular dynamics simulations of the ternary complex nisin2:lipid II. Scientific Reports, 6(21185), https://doi.org/10.1038/srep21185

Lanthionine antibiotics are an important class of naturally-occurring antimicrobial peptides. The best-known, nisin, is a commercial food preservative. However, structural and mechanistic details on nisin/lipid II membrane complexes are currently lac... Read More about Docking and molecular dynamics simulations of the ternary complex nisin2:lipid II.

New CHARMM force field parameters for dehydrated amino acid residues, the key to lantibiotic molecular dynamics simulations (2014)
Journal Article
Turpin, E. R., Mulholland, S., Teale, A. M., Bonev, B. B., & Hirst, J. D. (2014). New CHARMM force field parameters for dehydrated amino acid residues, the key to lantibiotic molecular dynamics simulations. RSC Advances, 4(89), 48621-48631. https://doi.org/10.1039/c4ra09897h

Lantibiotics are an important class of naturally occurring antimicrobial peptides containing unusual dehydrated amino acid residues. In order to enable molecular dynamics simulations of lantibiotics, we have developed empirical force field parameters... Read More about New CHARMM force field parameters for dehydrated amino acid residues, the key to lantibiotic molecular dynamics simulations.

Molecular mechanism of target recognition by subtilin, a class I lanthionine antibiotic (2008)
Journal Article

The increasing resistance of human pathogens to conventional antibiotics presents a growing threat to the chemotherapeutic management of infectious diseases. The lanthionine antibiotics, still unused as therapeutic agents, have recently attracted sig... Read More about Molecular mechanism of target recognition by subtilin, a class I lanthionine antibiotic.