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A conserved trypanosomatid differentiation regulator controls substrate attachment and morphological development in Trypanosoma congolense (2024)
Journal Article
Silvester, E., Szoor, B., Ivens, A., Awuah-Mensah, G., Gadelha, C., Wickstead, B., & Matthews, K. R. (2024). A conserved trypanosomatid differentiation regulator controls substrate attachment and morphological development in Trypanosoma congolense. PLoS Pathogens, 20(2), Article e1011889. https://doi.org/10.1371/journal.ppat.1011889

Trypanosomatid parasites undergo developmental regulation to adapt to the different environments encountered during their life cycle. In Trypanosoma brucei, a genome wide selectional screen previously identified a regulator of the protein family ESAG... Read More about A conserved trypanosomatid differentiation regulator controls substrate attachment and morphological development in Trypanosoma congolense.

A synthetic ancestral kinesin-13 depolymerizes microtubules faster than any natural depolymerizing kinesin (2022)
Journal Article
Belsham, H. R., Alghamdi, H. M., Dave, N., Rathbone, A. J., Wickstead, B., & Friel, C. T. (2022). A synthetic ancestral kinesin-13 depolymerizes microtubules faster than any natural depolymerizing kinesin. Open Biology, 12(8), Article 220133. https://doi.org/10.1098/rsob.220133

The activity of a kinesin is largely determined by the approximately 350 residue motor domain, and this region alone is sufficient to classify a kinesin as a member of a particular family. The kinesin-13 family are a group of microtubule depolymerizi... Read More about A synthetic ancestral kinesin-13 depolymerizes microtubules faster than any natural depolymerizing kinesin.

Divergent metabolism between Trypanosoma congolense and Trypanosoma brucei results in differential sensitivity to metabolic inhibition (2021)
Journal Article
Steketee, P. C., Dickie, E. A., Iremonger, J., Crouch, K., Paxton, E., Jayaraman, S., …Morrison, L. J. (2021). Divergent metabolism between Trypanosoma congolense and Trypanosoma brucei results in differential sensitivity to metabolic inhibition. PLoS Pathogens, 17(7), Article e1009734. https://doi.org/10.1371/journal.ppat.1009734

Animal African Trypanosomiasis (AAT) is a debilitating livestock disease prevalent across sub-Saharan Africa, a main cause of which is the protozoan parasite Trypanosoma congolense. In comparison to the well-studied T. brucei, there is a major paucit... Read More about Divergent metabolism between Trypanosoma congolense and Trypanosoma brucei results in differential sensitivity to metabolic inhibition.

TbSAP is a novel chromatin protein repressing metacyclic variant surface glycoprotein expression sites in bloodstream form Trypanosoma brucei (2021)
Journal Article
Davies, C., Ooi, C. P., Sioutas, G., Hall, B. S., Sidhu, H., Butter, F., …Rudenko, G. (2021). TbSAP is a novel chromatin protein repressing metacyclic variant surface glycoprotein expression sites in bloodstream form Trypanosoma brucei. Nucleic Acids Research, 49(6), 3242-3262. https://doi.org/10.1093/nar/gkab109

The African trypanosome Trypanosoma brucei is a unicellular eukaryote, which relies on a protective variant surface glycoprotein (VSG) coat for survival in the mammalian host. A single trypanosome has >2000 VSG genes and pseudogenes of which only one... Read More about TbSAP is a novel chromatin protein repressing metacyclic variant surface glycoprotein expression sites in bloodstream form Trypanosoma brucei.

Reliable, scalable functional genetics in bloodstream-form Trypanosoma congolense in vitro and in vivo (2021)
Journal Article
Awuah-Mensah, G., McDonald, J., Steketee, P. C., Autheman, D., Whipple, S., D'Archivio, S., …Wickstead, B. (2021). Reliable, scalable functional genetics in bloodstream-form Trypanosoma congolense in vitro and in vivo. PLoS Pathogens, 17(1), Article e1009224. https://doi.org/10.1371/journal.ppat.1009224

Copyright: © 2021 Awuah-Mensah et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author a... Read More about Reliable, scalable functional genetics in bloodstream-form Trypanosoma congolense in vitro and in vivo.

Plasmodium P-type cyclin CYC3 modulates endomitotic growth during oocyst development in mosquitoes (2015)
Journal Article
Roques, M., Wall, R. J., Douglass, A. P., Ramaprasad, A., Ferguson, D. J. P., Kaindama, M. L., …Tewari, R. (2015). Plasmodium P-type cyclin CYC3 modulates endomitotic growth during oocyst development in mosquitoes. PLoS Pathogens, 11(11), Article e1005273. https://doi.org/10.1371/journal.ppat.1005273

Cell-cycle progression and cell division in eukaryotes are governed in part by the cyclin family and their regulation of cyclin-dependent kinases (CDKs). Cyclins are very well characterised in model systems such as yeast and human cells, but surprisi... Read More about Plasmodium P-type cyclin CYC3 modulates endomitotic growth during oocyst development in mosquitoes.

Identification of the ISWI chromatin remodeling complex of the early branching Eukaryote Trypanosoma brucei (2015)
Journal Article
Stanne, T., Shankar Narayanan, M., Ridewood, S., Ling, A., Witmer, K., Kushwaha, M., …Rudenko, G. (2015). Identification of the ISWI chromatin remodeling complex of the early branching Eukaryote Trypanosoma brucei. Journal of Biological Chemistry, 290, https://doi.org/10.1074/jbc.M115.679019

ISWI chromatin remodelers are highly conserved in eukaryotes and are important for the assembly and spacing of nucleosomes, thereby controlling transcription initiation and elongation. ISWI is typically associated with different subunits, forming sp... Read More about Identification of the ISWI chromatin remodeling complex of the early branching Eukaryote Trypanosoma brucei.

A SAS-6-like protein suggests that the Toxoplasma conoid complex evolved from flagellar components (2013)
Journal Article
de Leon, J. C., Scheumann, N., Beatty, W., Beck, J. R., Tran, J. Q., Yau, C., …Morrissette, N. S. (2013). A SAS-6-like protein suggests that the Toxoplasma conoid complex evolved from flagellar components. Eukaryotic Cell, 12(7), https://doi.org/10.1128/EC.00096-13

SAS-6 is required for centriole biogenesis in diverse eukaryotes. Here, we describe a novel family of SAS-6-like (SAS6L) proteins that share an N-terminal domain with SAS-6 but lack coiled-coil tails. SAS6L proteins are found in a subset of eukaryote... Read More about A SAS-6-like protein suggests that the Toxoplasma conoid complex evolved from flagellar components.