All Outputs (3)

Rapid host strain improvement by in vivo rearrangement of a synthetic yeast chromosome (2018)
Journal Article
Blount, B. A., Gowers, G.-O. F., Ho, J. C. H., Ledesma-Amaro, R., Jovicevic, D., McKiernan, R. M., …Ellis, T. (2018). Rapid host strain improvement by in vivo rearrangement of a synthetic yeast chromosome. Nature Communications, 9(1), Article 1932. https://doi.org/10.1038/s41467-018-03143-w

© 2018 The Author(s). Synthetic biology tools, such as modular parts and combinatorial DNA assembly, are routinely used to optimise the productivity of heterologous metabolic pathways for biosynthesis or substrate utilisation, yet it is well establis... Read More about Rapid host strain improvement by in vivo rearrangement of a synthetic yeast chromosome.

Biosynthesis of the antibiotic nonribosomal peptide penicillin in baker’s yeast (2017)
Journal Article
Awan, A. R., Blount, B. A., Bell, D. J., Shaw, W. M., Ho, J. C., McKiernan, R. M., & Ellis, T. (2017). Biosynthesis of the antibiotic nonribosomal peptide penicillin in baker’s yeast. Nature Communications, 8, Article 15202. https://doi.org/10.1038/ncomms15202

© The Author(s) 2017. Fungi are a valuable source of enzymatic diversity and therapeutic natural products including antibiotics. Here we engineer the baker’s yeast Saccharomyces cerevisiae to produce and secrete the antibiotic penicillin, a beta-lact... Read More about Biosynthesis of the antibiotic nonribosomal peptide penicillin in baker’s yeast.

GC preps: Fast and easy extraction of stable yeast genomic DNA (2016)
Journal Article
Blount, B. A., Driessen, M. R., & Ellis, T. (2016). GC preps: Fast and easy extraction of stable yeast genomic DNA. Scientific Reports, 6, Article 26863. https://doi.org/10.1038/srep26863

Existing yeast genomic DNA extraction methods are not ideally suited to extensive screening of colonies by PCR, due to being too lengthy, too laborious or yielding poor quality DNA and inconsistent results. We developed the GC prep method as a soluti... Read More about GC preps: Fast and easy extraction of stable yeast genomic DNA.