Skip to main content

Research Repository

See what's under the surface

Advanced Search

Auxin methylation is required for differential growth in Arabidopsis

Abbas, Mohamad; Hernández-García, Jorge; Pollmann, Stephan; Samodelov, Sophia L.; Kolb, Martina; Friml, Jirí; Hammes, Ulrich Z.; Zurbriggen, Matías; Blázquez, Miguel A.; Alabadí, David

Authors

Mohamad Abbas

Jorge Hernández-García

Stephan Pollmann

Sophia L. Samodelov

Martina Kolb

Jirí Friml

Ulrich Z. Hammes

Matías Zurbriggen

Miguel A. Blázquez

David Alabadí



Abstract

Asymmetric auxin distribution is instrumental for the differential growth that causes organ bending on tropic stimuli and curvatures during plant development. Local differences in auxin concentrations are achieved mainly by polarized cellular distribution of PIN auxin transporters, but whether other mechanisms involving auxin homeostasis are also relevant for the formation of auxin gradients is not clear. Here we show that auxin methylation is required for asymmetric auxin distribution across the hypocotyl, particularly during its response to gravity. We found that loss-of-function mutants in Arabidopsis IAA CARBOXYL METHYLTRANSFERASE1 (IAMT1) prematurely unfold the apical hook, and that their hypocotyls are impaired in gravitropic reorientation. This defect is linked to an auxin-dependent increase in PIN gene expression, leading to an increased polar auxin transport and lack of asymmetric distribution of PIN3 in the iamt1 mutant. Gravitropic reorientation in the iamt1 mutant could be restored with either endodermis-specific expression of IAMT1 or partial inhibition of polar auxin transport, which also results in normal PIN gene expression levels. We propose that IAA methylation is necessary in gravity-sensing cells to restrict polar auxin transport within the range of auxin levels that allow for differential responses.

Journal Article Type Article
Publication Date Jun 13, 2018
Journal Proceedings of the National Academy of Sciences
Electronic ISSN 1091-6490
Publisher National Academy of Sciences
Peer Reviewed Peer Reviewed
Volume 115
Issue 26
Pages 6864-6869
APA6 Citation Abbas, M., Hernández-García, J., Pollmann, S., Samodelov, S. L., Kolb, M., Friml, J., …Alabadí, D. (2018). Auxin methylation is required for differential growth in Arabidopsis. Proceedings of the National Academy of Sciences, 115(26), 6864-6869. doi:10.1073/pnas.1806565115
DOI https://doi.org/10.1073/pnas.1806565115
Publisher URL http://www.pnas.org/content/early/2018/06/07/1806565115
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

Files

pnas201806565_1wxasn.pdf (1.2 Mb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf


pnas201806565_1wxasn.pdf (1.3 Mb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





You might also like



Downloadable Citations

;