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Increased maternal and fetal cholesterol efflux capacity and placental cyp27a1 expression in pre-eclampsia

Mistry, Hiten D.; Kurlak, L.O.; Mansour, Yosef; Zurkinden, Line; Mohaupt, Markus; Escher, Genevieve

Authors

Hiten D. Mistry hiten.mistry@nottingham.ac.uk

L.O. Kurlak lesia.kurlak@nottingham.ac.uk

Yosef Mansour

Line Zurkinden

Markus Mohaupt

Genevieve Escher



Abstract

Preeclampsia is a pregnancy-specific condition that leads to increased cardiovascular risk in later life. A decrease in cholesterol efflux capacity is linked to CVD. We hypothesized that in preeclampsia there would be a disruption of maternal/fetal plasma to efflux cholesterol, as well as differences in the concentrations of both placental sterol 27-hydroxylase (CYP27A1) and apoA1 binding protein (AIBP). Total, HDL-, and ABCA1-mediated cholesterol effluxes were performed with maternal and fetal plasma from women with preeclampsia and normotensive controls (both n = 17). apoA1 and apoE were quantified by chemiluminescence, and 27-hydroxycholesterol (27-OHC) by GC-MS. Immunohistochemistry was used to determine placental expression/localization of CYP27A1, AIBP, apoA1, apoE, and SRB1. Maternal and fetal total and HDL-mediated cholesterol efflux capacities were increased in preeclampsia (by 10–20%), but ABCA1-mediated efflux was decreased (by 20–35%; P < 0.05). Maternal and fetal apoE concentrations were higher in preeclampsia. Fetal plasma 27-OHC levels were decreased in preeclamptic samples (P < 0.05). Placental protein expression of both CYP27A1 and AIBP were localized around fetal vessels and significantly increased in preeclampsia (P = 0.04). Placental 27-OHC concentrations were also raised in preeclampsia (P < 0.05). Increased HDL-mediated cholesterol efflux capacity and placental CYP27A1/27-OHC could be a rescue mechanism in preeclampsia, to remove cholesterol from cells to limit lipid peroxidation and increase placental angiogenesis.

Journal Article Type Article
Publication Date Jun 10, 2017
Journal Journal of Lipid Research
Print ISSN 0022-2275
Electronic ISSN 1539-7262
Publisher American Society for Biochemistry and Molecular Biology
Peer Reviewed Peer Reviewed
Volume 58
APA6 Citation Mistry, H. D., Kurlak, L., Mansour, Y., Zurkinden, L., Mohaupt, M., & Escher, G. (2017). Increased maternal and fetal cholesterol efflux capacity and placental cyp27a1 expression in pre-eclampsia. Journal of Lipid Research, 58, doi:10.1194/jlr.M071985
DOI https://doi.org/10.1194/jlr.M071985
Keywords lipid/efflux; 27-hydroxycholesterol; apolipoproteins; pregnancy; hypertension; sterol 27-hydroxylase
Publisher URL http://www.jlr.org/content/58/6/1186.short
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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CE and Plac CYP paper Final accpeted.pdf (753 Kb)
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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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