Study to investigate the prevalence of human papillomavirus in Barrett's oesophagus using a novel screening methodology

White, Jonathan Richard; Ragunath, Krish; Whitton, Aimee; Marsh, Elizabeth; Kaye, Philip; Knight, Gillian

Abstract

Introduction Human papillomavirus (HPV) is strongly associated with Barrett's dysplasia and oesophageal cancer suggesting a role in carcinogenesis. HPV persistence predicts treatment failure after endotherapy for Barrett's dysplasia. This pilot study applies a novel HPV screening tool (previously only used in the oropharynx) to detect HPV DNA directly and determine the prevalence rates in Barrett's oesophagus (BO). Method DNA was extracted from 20 formalin-fixed BO samples. HPV DNA was detected using real-time PCR and gel electrophoresis. Results 5 out of 20 patients were identified as positive for HPV. Prevalence was 25% in patients with BO. Conclusion This method can be used in BO's tissue to determine HPV infection. Adoption of this as a screening test could potentially revolutionise future research in this area. If a clear link between HPV and Barrett's dysplasia can be confirmed, this qPCR method has the potential to aid in monitoring and/or dysplasia detection by stratifying those most at risk and aid in the development of new therapies.

Citation

White, J. R., Ragunath, K., Whitton, A., Marsh, E., Kaye, P., & Knight, G. (2022). Study to investigate the prevalence of human papillomavirus in Barrett's oesophagus using a novel screening methodology. BMJ Open Gastroenterology, 9(1), Article e000840. https://doi.org/10.1136/bmjgast-2021-000840

• Journal Article Type: Article
• Acceptance Date: Jan 25, 2022
• Online Publication Date: Apr 4, 2022
• Publication Date: Apr 1, 2022
• Deposit Date: Apr 7, 2022
• Publicly Available Date: Apr 8, 2022
• Journal: BMJ Open Gastroenterology
• Electronic ISSN: 2054-4774
• Publisher: BMJ Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 9
• Issue: 1
• Article Number: e000840
• DOI: https://doi.org/10.1136/bmjgast-2021-000840
• Keywords: Gastroenterology
• Public URL: https://nottingham-repository.worktribe.com/output/7711671
• Publisher URL: https://bmjopengastro.bmj.com/content/9/1/e000840