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Host adaption to the bacteriophage carrier state of Campylobacter jejuni

Brathwaite, Kelly J.; Siringan, Patcharin; Connerton, Phillippa L.; Connerton, Ian F.

Authors

Kelly J. Brathwaite

Patcharin Siringan

Phillippa L. Connerton

IAN CONNERTON IAN.CONNERTON@NOTTINGHAM.AC.UK
Northern Foods Professor of Food Safety



Abstract

The carrier state of the foodborne pathogen Campylobacter jejuni represents an alternative life cycle whereby virulent bacteriophage can persistent in association with host bacteria without commitment to lysogeny. Host bacteria exhibit significant phenotypic changes that improve their ability to survive extra-intestinal environments but exhibit growth phase dependent impairment in motility. We demonstrate that early-exponential phase cultures become synchronised with respect to the non-motile phenotype, which corresponds with a reduction in their ability adhere and invade intestinal epithelial cells. Comparative transcriptome analyses (RNA-seq) identify changes in gene expression that account for the observed phenotypes: down regulation of stress response genes hrcA, hspR and perR; and down regulation of the major flagellin flaA with the chemotactic response signalling genes cheV, cheA and cheW. These changes present mechanisms by which the host and bacteriophage can remain associated without lysis, and the cultures survive extra-intestinal transit. These data provide a basis for understanding a critical link in the ecology of Campylobacter bacteriophage.

Citation

Brathwaite, K. J., Siringan, P., Connerton, P. L., & Connerton, I. F. (2015). Host adaption to the bacteriophage carrier state of Campylobacter jejuni. Research in Microbiology, 166(6), 504-515. https://doi.org/10.1016/j.resmic.2015.05.003

Journal Article Type Article
Acceptance Date May 6, 2015
Online Publication Date May 22, 2015
Publication Date 2015-07
Deposit Date Jul 20, 2015
Publicly Available Date Jul 20, 2015
Journal Research in Microbiology
Print ISSN 0923-2508
Electronic ISSN 0923-2508
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 166
Issue 6
Pages 504-515
DOI https://doi.org/10.1016/j.resmic.2015.05.003
Keywords Campylobacter, bacteriophage, carrier state life cycle, RNA-seq
Public URL http://eprints.nottingham.ac.uk/id/eprint/28911
Publisher URL http://www.sciencedirect.com/science/article/pii/S0923250815000789
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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