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Investigating the effects of an oral fructose challenge on hepatic ATP reserves in healthy volunteers: a 31P MRS study

Bawden, Stephen; Stephenson, M.C.; Ciampi, Elisabetta; Hunter, K.; Marciani, Luca; MacDonald, Ian A.; Aithal, Guruprasad P.; Morris, P.G.; Gowland, Penny A.

Authors

M.C. Stephenson

Elisabetta Ciampi

K. Hunter

LUCA MARCIANI LUCA.MARCIANI@NOTTINGHAM.AC.UK
Professor of Gastrointestinal Imaging

Ian A. MacDonald

Guruprasad P. Aithal

P.G. Morris

Penny A. Gowland



Abstract

Background: Impaired homeostasis of hepatic ATP has been associated with NAFLD. An intravenous fructose infusion has been shown to be an effective challenge to monitor the depletion and subsequent recovery of hepatic ATP reserves using 31P MRS.
Aims: The purpose of this study was to evaluate the effects of an oral rather than intravenous fructose challenge on hepatic ATP reserves in healthy subjects.
Methods: Self-reported healthy males were recruited. Following an overnight fast, baseline liver glycogen and lipid levels were measured using Magnetic Resonance Spectroscopy (MRS). Immediately after consuming a 500 ml 75 g fructose drink (1275 kJ) subjects were scanned continuously for 90 min to acquire dynamic 31P MRS measurements of liver ATP reserves.
Results: A significant effect on ATP reserves was observed across the time course (P < 0.05). Mean ATP levels reached a minimum at 50 min which was markedly lower than baseline (80 ± 17% baseline, P < 0.05). Subsequently, mean values tended to rise but did not reach statistical significance above minimum. The time to minimum ATP levels across subjects was negatively correlated with BMI (R2 ¼ 0.74, P < 0.005). Rates of ATP recovery were not significantly correlated with BMI or liver fat levels, but were negatively correlated with baseline glycogen levels (R2 ¼ 0.7, P < 0.05).
Conclusions: Depletion of ATP reserves can be measured non-invasively following an oral fructose challenge using 31P.

Journal Article Type Article
Journal Clinical Nutrition
Print ISSN 0261-5614
Electronic ISSN 1532-1983
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 35
Issue 3
APA6 Citation Bawden, S., Stephenson, M., Ciampi, E., Hunter, K., Marciani, L., MacDonald, I. A., …Gowland, P. A. (in press). Investigating the effects of an oral fructose challenge on hepatic ATP reserves in healthy volunteers: a 31P MRS study. Clinical Nutrition, 35(3), https://doi.org/10.1016/j.clnu.2015.04.001
DOI https://doi.org/10.1016/j.clnu.2015.04.001
Keywords ATP, fructose infusion, oral challenge, NAFLD, liver, 31P MRS
Publisher URL http://www.sciencedirect.com/science/article/pii/S0261561415000990
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0





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