Effects of cyclic equibiaxial mechanical stretch on α-BK and TRPV4 expression in equine chondrocytes

Hdud, Ismail M.; Mobasheri, Ali; Loughna, Paul

Abstract

Background

Chondrocytes are regularly exposed to load-induced stimuli and have the capability to sense and respond to applied mechanical stress. However, the mechanisms involved in chondrocyte mechanotransduction are not clearly understood. The purpose of this study was to explore the effects of cyclic equibiaxial mechanical stretch on the expression of α-BK and TRPV4 channels.
Findings

Freshly isolated equine articular chondrocytes were subjected to mechanical stress (8% elongation at frequency of 0.5 Hz for 8 h). Western blotting was used to investigate the expression of BKCa and TRPV4 channel proteins. Mechanical stretch increased the expression of BKCa channels by 1.8 fold but TRPV4 expression was not affected.
Conclusions

Upregulation of BKCa channel may be the result of direct membrane stretch or elevated intracellular Ca2+.

Citation

Hdud, I. M., Mobasheri, A., & Loughna, P. (2014). Effects of cyclic equibiaxial mechanical stretch on α-BK and TRPV4 expression in equine chondrocytes. SpringerPlus, 3, Article 59. https://doi.org/10.1186/2193-1801-3-59

• Journal Article Type: Article
• Acceptance Date: Jan 21, 2014
• Publication Date: Jan 29, 2014
• Deposit Date: Sep 25, 2017
• Publicly Available Date: Sep 25, 2017
• Journal: SpringerPlus
• Electronic ISSN: 2193-1801
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 3
• Article Number: 59
• DOI: https://doi.org/10.1186/2193-1801-3-59
• Keywords: Chondrocyte; Mechanical stretch; TRPV4; BK channel; Mechanotransduction
• Public URL: https://nottingham-repository.worktribe.com/output/721007
• Publisher URL: https://springerplus.springeropen.com/articles/10.1186/2193-1801-3-59
• Contract Date: Sep 25, 2017