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Effects of cyclic equibiaxial mechanical stretch on α-BK and TRPV4 expression in equine chondrocytes

Hdud, Ismail M.; Mobasheri, Ali; Loughna, Paul

Authors

Ismail M. Hdud mrxih2@nottingham.ac.uk

Ali Mobasheri

Paul Loughna paul.loughna@nottingham.ac.uk

Abstract

Background

Chondrocytes are regularly exposed to load-induced stimuli and have the capability to sense and respond to applied mechanical stress. However, the mechanisms involved in chondrocyte mechanotransduction are not clearly understood. The purpose of this study was to explore the effects of cyclic equibiaxial mechanical stretch on the expression of α-BK and TRPV4 channels.
Findings

Freshly isolated equine articular chondrocytes were subjected to mechanical stress (8% elongation at frequency of 0.5 Hz for 8 h). Western blotting was used to investigate the expression of BKCa and TRPV4 channel proteins. Mechanical stretch increased the expression of BKCa channels by 1.8 fold but TRPV4 expression was not affected.
Conclusions

Upregulation of BKCa channel may be the result of direct membrane stretch or elevated intracellular Ca2+.

Journal Article Type Article
Publication Date Jan 29, 2014
Journal SpringerPlus
Electronic ISSN 2193-1801
Publisher Humana Press
Peer Reviewed Peer Reviewed
Volume 3
Article Number 59
Institution Citation Hdud, I. M., Mobasheri, A., & Loughna, P. (2014). Effects of cyclic equibiaxial mechanical stretch on α-BK and TRPV4 expression in equine chondrocytes. SpringerPlus, 3, doi:10.1186/2193-1801-3-59
DOI https://doi.org/10.1186/2193-1801-3-59
Keywords Chondrocyte; Mechanical stretch; TRPV4; BK channel; Mechanotransduction
Publisher URL https://springerplus.springeropen.com/articles/10.1186/2193-1801-3-59
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0




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