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Digalactoside expression in the lipopolysaccharide of haemophilus influenzae and its role in intravascular survival

Griffin, R.; Bayliss, Christopher D.; Herbert, Mark A.; Cox, Andrew D.; Makepeace, Katherine; Richards, J.C.; Hood, Derek W.; Moxon, E. Richard

Authors

R. Griffin mbzrg@nottingham.ac.uk

Christopher D. Bayliss

Mark A. Herbert

Andrew D. Cox

Katherine Makepeace

J.C. Richards

Derek W. Hood

E. Richard Moxon



Abstract

Digalactoside (gal-1-4 gal) structures of the lipopolysaccharide (LPS) of Haemophilus influenzae are implicated in virulence. A confounding factor is that tetranucleotide repeats within the lic2A, lgtC, and lex2 genes mediate phase-variable expression of the digalactosides. By deleting these repeats, we constructed recombinant strains of RM153 constitutively expressing either one or two LPS digalactosides. Expression of two digalactosides, rather than one, was associated with increased virulence of H. influenzae in vivo.

Journal Article Type Article
Publication Date Oct 1, 2005
Journal Infection and Immunity
Print ISSN 0019-9567
Electronic ISSN 1098-5522
Publisher American Society for Microbiology
Peer Reviewed Peer Reviewed
Volume 73
Issue 10
APA6 Citation Griffin, R., Bayliss, C. D., Herbert, M. A., Cox, A. D., Makepeace, K., Richards, J., …Moxon, E. R. (2005). Digalactoside expression in the lipopolysaccharide of haemophilus influenzae and its role in intravascular survival. Infection and Immunity, 73(10), doi:10.1128/IAI.73.10.7022-7026.2005
DOI https://doi.org/10.1128/IAI.73.10.7022-7026.2005
Publisher URL http://iai.asm.org/content/73/10/7022
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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