Digalactoside expression in the lipopolysaccharide of haemophilus influenzae and its role in intravascular survival

Griffin, R.; Bayliss, Christopher D.; Herbert, Mark A.; Cox, Andrew D.; Makepeace, Katherine; Richards, J.C.; Hood, Derek W.; Moxon, E. Richard

Abstract

Digalactoside (gal-1-4 gal) structures of the lipopolysaccharide (LPS) of Haemophilus influenzae are implicated in virulence. A confounding factor is that tetranucleotide repeats within the lic2A, lgtC, and lex2 genes mediate phase-variable expression of the digalactosides. By deleting these repeats, we constructed recombinant strains of RM153 constitutively expressing either one or two LPS digalactosides. Expression of two digalactosides, rather than one, was associated with increased virulence of H. influenzae in vivo.

Citation

Griffin, R., Bayliss, C. D., Herbert, M. A., Cox, A. D., Makepeace, K., Richards, J., Hood, D. W., & Moxon, E. R. (2005). Digalactoside expression in the lipopolysaccharide of haemophilus influenzae and its role in intravascular survival. Infection and Immunity, 73(10), https://doi.org/10.1128/IAI.73.10.7022-7026.2005

• Journal Article Type: Article
• Acceptance Date: Jun 16, 2005
• Publication Date: Oct 1, 2005
• Deposit Date: Dec 14, 2017
• Journal: Infection and Immunity
• Print ISSN: 0019-9567
• Electronic ISSN: 1098-5522
• Publisher: American Society for Microbiology
• Peer Reviewed: Peer Reviewed
• Volume: 73
• Issue: 10
• DOI: https://doi.org/10.1128/IAI.73.10.7022-7026.2005
• Public URL: https://nottingham-repository.worktribe.com/output/703428
• Publisher URL: http://iai.asm.org/content/73/10/7022
• Contract Date: Dec 12, 2017