Giuseppe Silvestri
Preimplantation genetic testing for aneuploidy improves live birth rates with in vitro produced bovine embryos: A blind retrospective study
Silvestri, Giuseppe; Canedo-Ribeiro, Carla; Serrano-Albal, María; Labrecque, Remi; Blondin, Patrick; Larmer, Steven G.; Marras, Gabriele; Tutt, Desmond A.R.; Handyside, Alan H.; Farré, Marta; Sinclair, Kevin D.; Griffin, Darren K.
Authors
Carla Canedo-Ribeiro
María Serrano-Albal
Remi Labrecque
Patrick Blondin
Steven G. Larmer
Gabriele Marras
Desmond A.R. Tutt
Alan H. Handyside
Marta Farré
KEVIN SINCLAIR kevin.sinclair@nottingham.ac.uk
Professor of Developmental Biology
Darren K. Griffin
Abstract
Approximately one million in vitro produced (IVP) cattle embryos are transferred world-wide each year as a way to improve the rates of genetic gain. The most advanced programmes also apply genomic selection at the embryonic stage by SNP genotyping and the calculation of genomic estimated breeding values (GEBVs). However, a high proportion of cattle embryos fail to establish a pregnancy. Here, we demonstrate that further interrogation of the SNP data collected for GEBVs can effectively remove aneuploid embryos from the pool, improving live births per embryo transfer (ET). Using three preimplantation genetic testing for aneuploidy (PGT-A) approaches, we assessed 1713 cattle blastocysts in a blind, retrospective analysis. Our findings indicate aneuploid embryos have a 5.8% chance of establishing a pregnancy and a 5.0% chance of given rise to a live birth. This compares to 59.6% and 46.7% for euploid embryos (p < 0.0001). PGT-A improved overall pregnancy and live birth rates by 7.5% and 5.8%, respectively (p < 0.0001). More detailed analyses revealed donor, chromosome, stage, grade, and sex-specific rates of error. Notably, we discovered a significantly higher incidence of aneuploidy in XY embryos and, as in humans, detected a preponderance of maternal meiosis I errors. Our data strongly support the use of PGT-A in cattle IVP programmes.
Citation
Silvestri, G., Canedo-Ribeiro, C., Serrano-Albal, M., Labrecque, R., Blondin, P., Larmer, S. G., …Griffin, D. K. (2021). Preimplantation genetic testing for aneuploidy improves live birth rates with in vitro produced bovine embryos: A blind retrospective study. Cells, 10(9), Article 2284. https://doi.org/10.3390/cells10092284
Journal Article Type | Article |
---|---|
Acceptance Date | Aug 30, 2021 |
Online Publication Date | Sep 2, 2021 |
Publication Date | Sep 1, 2021 |
Deposit Date | Mar 28, 2022 |
Publicly Available Date | Mar 28, 2024 |
Journal | Cells |
Electronic ISSN | 2073-4409 |
Publisher | MDPI AG |
Peer Reviewed | Peer Reviewed |
Volume | 10 |
Issue | 9 |
Article Number | 2284 |
DOI | https://doi.org/10.3390/cells10092284 |
Keywords | General Medicine |
Public URL | https://nottingham-repository.worktribe.com/output/6184454 |
Publisher URL | https://www.mdpi.com/2073-4409/10/9/2284 |
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Silvestri Et Al 2021 - Cells
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Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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