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Preimplantation genetic testing for aneuploidy improves live birth rates with in vitro produced bovine embryos: A blind retrospective study

Silvestri, Giuseppe; Canedo-Ribeiro, Carla; Serrano-Albal, María; Labrecque, Remi; Blondin, Patrick; Larmer, Steven G.; Marras, Gabriele; Tutt, Desmond A.R.; Handyside, Alan H.; Farré, Marta; Sinclair, Kevin D.; Griffin, Darren K.

Preimplantation genetic testing for aneuploidy improves live birth rates with in vitro produced bovine embryos: A blind retrospective study Thumbnail


Authors

Giuseppe Silvestri

Carla Canedo-Ribeiro

María Serrano-Albal

Remi Labrecque

Patrick Blondin

Steven G. Larmer

Gabriele Marras

Desmond A.R. Tutt

Alan H. Handyside

Marta Farré

KEVIN SINCLAIR kevin.sinclair@nottingham.ac.uk
Professor of Developmental Biology

Darren K. Griffin



Abstract

Approximately one million in vitro produced (IVP) cattle embryos are transferred world-wide each year as a way to improve the rates of genetic gain. The most advanced programmes also apply genomic selection at the embryonic stage by SNP genotyping and the calculation of genomic estimated breeding values (GEBVs). However, a high proportion of cattle embryos fail to establish a pregnancy. Here, we demonstrate that further interrogation of the SNP data collected for GEBVs can effectively remove aneuploid embryos from the pool, improving live births per embryo transfer (ET). Using three preimplantation genetic testing for aneuploidy (PGT-A) approaches, we assessed 1713 cattle blastocysts in a blind, retrospective analysis. Our findings indicate aneuploid embryos have a 5.8% chance of establishing a pregnancy and a 5.0% chance of given rise to a live birth. This compares to 59.6% and 46.7% for euploid embryos (p < 0.0001). PGT-A improved overall pregnancy and live birth rates by 7.5% and 5.8%, respectively (p < 0.0001). More detailed analyses revealed donor, chromosome, stage, grade, and sex-specific rates of error. Notably, we discovered a significantly higher incidence of aneuploidy in XY embryos and, as in humans, detected a preponderance of maternal meiosis I errors. Our data strongly support the use of PGT-A in cattle IVP programmes.

Citation

Silvestri, G., Canedo-Ribeiro, C., Serrano-Albal, M., Labrecque, R., Blondin, P., Larmer, S. G., …Griffin, D. K. (2021). Preimplantation genetic testing for aneuploidy improves live birth rates with in vitro produced bovine embryos: A blind retrospective study. Cells, 10(9), Article 2284. https://doi.org/10.3390/cells10092284

Journal Article Type Article
Acceptance Date Aug 30, 2021
Online Publication Date Sep 2, 2021
Publication Date Sep 1, 2021
Deposit Date Mar 28, 2022
Publicly Available Date Mar 28, 2024
Journal Cells
Electronic ISSN 2073-4409
Publisher MDPI AG
Peer Reviewed Peer Reviewed
Volume 10
Issue 9
Article Number 2284
DOI https://doi.org/10.3390/cells10092284
Keywords General Medicine
Public URL https://nottingham-repository.worktribe.com/output/6184454
Publisher URL https://www.mdpi.com/2073-4409/10/9/2284