Depletion of HP1α alters the mechanical properties of MCF7 nuclei

Pradhan, Susav; Solomon, Raoul; Gangotra, Ankita; Yakubov, Gleb E.; Willmott, Geoff R.; Whitby, Catherine P.; Hale, Tracy K.; Williams, Martin A.K.

Abstract

Within the nucleus of the eukaryotic cell, DNA is partitioned into domains of highly condensed, transcriptionally silent heterochromatin and less condensed, transcriptionally active euchromatin. Heterochromatin protein 1α (HP1α) is an architectural protein that establishes and maintains heterochromatin, ensuring genome fidelity and nuclear integrity. Although the mechanical effects of changes in the relative amount of euchromatin and heterochromatin brought about by inhibiting chromatin-modifying enzymes have been studied previously, here we measure how the material properties of the nuclei are modified after the knockdown of HP1α. These studies were inspired by the observation that poorly invasive MCF7 breast cancer cells become more invasive after knockdown of HP1α expression and that, indeed, in many solid tumors the loss of HP1α correlates with the onset of tumor cell invasion. Atomic force microscopy (AFM), optical tweezers (OT), and techniques based on micropipette aspiration (MA) were each used to characterize the mechanical properties of nuclei extracted from HP1α knockdown or matched control MCF7 cells. Using AFM or OT to locally indent nuclei, those extracted from MCF7 HP1α knockdown cells were found to have apparent Young's moduli that were significantly lower than nuclei from MCF7 control cells, consistent with previous studies that assert heterochromatin plays a major role in governing the mechanical response in such experiments. In contrast, results from pipette-based techniques in the spirit of MA, in which the whole nuclei were deformed and aspirated into a conical pipette, showed considerably less variation between HP1α knockdown and control, consistent with previous studies reporting that it is predominantly the lamins in the nuclear envelope that determine the mechanical response to large whole-cell deformations. The differences in chromatin organization observed by various microscopy techniques between the MCF7 control and HP1α knockdown nuclei correlate well with the results of our measured mechanical responses and our hypotheses regarding their origin.

Citation

Pradhan, S., Solomon, R., Gangotra, A., Yakubov, G. E., Willmott, G. R., Whitby, C. P., …Williams, M. A. (2021). Depletion of HP1α alters the mechanical properties of MCF7 nuclei. Biophysical Journal, 120(13), 2631-2643. https://doi.org/10.1016/j.bpj.2021.05.017

• Journal Article Type: Article
• Acceptance Date: May 15, 2021
• Online Publication Date: Jul 6, 2021
• Publication Date: Jul 6, 2021
• Deposit Date: Apr 15, 2023
• Journal: Biophysical Journal
• Print ISSN: 0006-3495
• Electronic ISSN: 1542-0086
• Publisher: Biophysical Society
• Peer Reviewed: Peer Reviewed
• Volume: 120
• Issue: 13
• Pages: 2631-2643
• DOI: https://doi.org/10.1016/j.bpj.2021.05.017
• Public URL: https://nottingham-repository.worktribe.com/output/5769294
• Publisher URL: https://www.cell.com/biophysj/fulltext/S0006-3495(21)00446-X