Comparing PCR techniques against conventional cercarial shedding methods for detecting Schistosoma mansoni infection in Biomphalaria snails

Joof, Ebrima; Andrus, Peter S.; Sowunmi, Kehinde; Onyango, Victor M.; Wade, Christopher M.

Abstract

© 2020 Elsevier B.V. The detection of Schistosoma mansoni infection in both its intermediate (snail) and definitive (human) hosts is useful in providing information on the transmission of schistosomiasis. Three pairs of previously designed PCR primers (SM1-7, SMF/R & ND5) used for the detection of S. mansoni infection were tested. We assess the utility of each of these primer sets for detecting S. mansoni infection both in artificially exposed laboratory bred Biomphalaria glabrata, and field infected African Biomphalaria sudanica and Biomphalaria pfeifferi. Two of the three primer sets (SMF/R & ND5) detected S. mansoni infection in snails, but amplification of S. mansoni DNA with SM1-7 was unreliable. For the artificially exposed laboratory bred B. glabrata snails, SMF/R and ND5 both detected infection in more snails than the cercarial shedding method. Infection detection rates were 62.4% for ND5, 57.1% for SMF/R and 50.4% using traditional cercarial shedding methods. Both SMF/R and ND5 detected S. mansoni infection in 91% of snails observed shedding cercariae, increasing to 98.5% when low stringency PCR methods were used. When comparing each of the detection methods using a Bayesian latent class analysis model, ND5 had the highest detection sensitivity and negative predictive value (NPV), while SMF/R had the highest detection specificity and positive predictive value (PPV). In field collected Biomphalaria snails, ND5 detected S. mansoni infection in 21 of 24 snails categorised as shedding S. mansoni cercariae and 4 of 24 snails categorised as shedding non-S. mansoni cercariae, while SMF/R detected infection in 18 of 24 snails categorised as shedding S. mansoni cercariae and in 3 of 24 snails categorised as shedding non-S. mansoni cercariae. All SMF/R and ND5 PCR products were shown to be S. mansoni indicating that these field snails must have been infected with both S. mansoni and cercariae from other Schistosoma species. This indicates that the two primer sets are specific for S. mansoni and will not amplify non-S. mansoni species when used at their recommended annealing temperatures. Both the SMF/R and ND5 primers effectively detected S. mansoni infection in three Biomphalaria species and have improved detection sensitivity over cercarial shedding.

Citation

Joof, E., Andrus, P. S., Sowunmi, K., Onyango, V. M., & Wade, C. M. (2020). Comparing PCR techniques against conventional cercarial shedding methods for detecting Schistosoma mansoni infection in Biomphalaria snails. Acta Tropica, 212, Article 105716. https://doi.org/10.1016/j.actatropica.2020.105716

• Journal Article Type: Article
• Acceptance Date: Sep 18, 2020
• Online Publication Date: Sep 20, 2020
• Publication Date: Dec 1, 2020
• Deposit Date: Aug 8, 2022
• Journal: Acta Tropica
• Print ISSN: 0001-706X
• Electronic ISSN: 1873-6254
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 212
• Article Number: 105716
• DOI: https://doi.org/10.1016/j.actatropica.2020.105716
• Public URL: https://nottingham-repository.worktribe.com/output/4958179
• Publisher URL: https://www.sciencedirect.com/science/article/abs/pii/S0001706X19315967?via%3Dihub