Jessica Buck
Sensitivity of Multiphase Pseudocontinuous Arterial Spin Labelling (MP pCASL) Magnetic Resonance Imaging for Measuring Brain and Tumour Blood Flow in Mice
Buck, Jessica; Larkin, James R.; Simard, Manon A.; Khrapitchev, Alexandre A.; Chappell, Michael A.; Sibson, Nicola R.
Authors
James R. Larkin
Manon A. Simard
Alexandre A. Khrapitchev
Prof MICHAEL CHAPPELL MICHAEL.CHAPPELL@NOTTINGHAM.AC.UK
Professor of Biomedical Imaging
Nicola R. Sibson
Abstract
Brain and tumour blood flow can be measured noninvasively using arterial spin labelling (ASL) magnetic resonance imaging (MRI), but reliable quantification in mouse models remains difficult. Pseudocontinuous ASL (pCASL) is recommended as the clinical standard for ASL and can be improved using multiphase labelling (MP pCASL). The aim of this study was to optimise and validate MP pCASL MRI for cerebral blood flow (CBF) measurement in mice and to assess its sensitivity to tumour perfusion. Following optimization of the MP pCASL sequence, CBF data were compared with gold-standard autoradiography, showing close agreement. Subsequently, MP pCASL data were acquired at weekly intervals in models of primary and secondary brain tumours, and tumour microvessel density was determined histologically. MP pCASL measurements in a secondary brain tumour model revealed a significant reduction in blood flow at day 35 after induction, despite a higher density of blood vessels. Tumour core regions also showed reduced blood flow compared with the tumour rim. Similarly, significant reductions in CBF were found in a model of glioma 28 days after tumour induction, together with an increased density of blood vessels. These findings indicate that MP pCASL MRI provides accurate and robust measurements of cerebral blood flow in naïve mice and is sensitive to changes in tumour perfusion.
Citation
Buck, J., Larkin, J. R., Simard, M. A., Khrapitchev, A. A., Chappell, M. A., & Sibson, N. R. (2018). Sensitivity of Multiphase Pseudocontinuous Arterial Spin Labelling (MP pCASL) Magnetic Resonance Imaging for Measuring Brain and Tumour Blood Flow in Mice. Contrast Media and Molecular Imaging, 2018, 1-11. https://doi.org/10.1155/2018/4580919
Journal Article Type | Article |
---|---|
Acceptance Date | Sep 26, 2018 |
Online Publication Date | Nov 7, 2018 |
Publication Date | Nov 7, 2018 |
Deposit Date | Sep 28, 2020 |
Publicly Available Date | Mar 15, 2021 |
Journal | Contrast Media & Molecular Imaging |
Print ISSN | 1555-4309 |
Electronic ISSN | 1555-4317 |
Publisher | Hindawi |
Peer Reviewed | Peer Reviewed |
Volume | 2018 |
Article Number | 4580919 |
Pages | 1-11 |
DOI | https://doi.org/10.1155/2018/4580919 |
Keywords | Radiology Nuclear Medicine and imaging |
Public URL | https://nottingham-repository.worktribe.com/output/4930874 |
Publisher URL | https://www.hindawi.com/journals/cmmi/2018/4580919/ |
Files
4580919
(3.8 Mb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
You might also like
Identifying the ischaemic penumbra using pH-weighted magnetic resonance imaging
(2014)
Journal Article
Model-based Bayesian inference of brain oxygenation using quantitative BOLD
(2019)
Journal Article
Association of Midlife Cardiovascular Risk Profiles with Cerebral Perfusion at Older Ages
(2019)
Journal Article
ICA-based denoising for ASL perfusion imaging
(2019)
Journal Article
Downloadable Citations
About Repository@Nottingham
Administrator e-mail: discovery-access-systems@nottingham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search