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The Arabidopsis thaliana N-recognin E3 ligase PROTEOLYSIS1 influences the immune response

Till, Christopher J.; Vicente, Jorge; Zhang, Hongtao; Oszvald, Maria; Deery, Michael J.; Pastor, Victoria; Lilley, Kathryn S.; Ray, Rumiana V.; Theodoulou, Frederica L.; Holdsworth, Michael J.

Authors

Christopher J. Till

Hongtao Zhang

Maria Oszvald

Michael J. Deery

Victoria Pastor

Kathryn S. Lilley

Frederica L. Theodoulou



Abstract

N-degron pathways of ubiquitin-mediated proteolysis (formerly known as the N-end rule pathway) control the stability of substrate proteins dependent on the amino-terminal (Nt) residue. Unlike yeast or mammalian N-recognin E3 ligases, which each recognize several different classes of Nt residues, in Arabidopsis thaliana, N-recognin functions of different N-degron pathways are carried out independently by PROTEOLYSIS (PRT)1, PRT6 and other unknown proteins. PRT1 recognizes type 2 aromatic Nt destabilizing residues and PRT6 recognizes type 1 basic residues. These two N-recognin functions diverged as separate proteins early in the evolution of plants, before the conquest of the land. We demonstrate that loss of PRT1 function promotes the plant immune system, as mutant prt1-1 plants showed greater apoplastic resistance than WT to infection by the bacterial hemi-biotroph Pseudomonas syringae pv tomato (Pst) DC3000. Quantitative proteomics revealed increased accumulation of proteins associated with specific components of plant defense in the prt1-1 mutant, concomitant with increased accumulation of salicylic acid. The effects of the prt1 mutation were additional to known effects of prt6 in influencing the immune system, in particular, an observed over-accumulation of pipecolic acid (Pip) in the double mutant prt1-1 prt6-1. These results demonstrate a potential role for PRT1 in controlling aspects ofthe plant immune system and suggest that PRT1 limits the onset of the defense response via degradation of substrates with type 2 Nt-destabilizing residues

Citation

Till, C. J., Vicente, J., Zhang, H., Oszvald, M., Deery, M. J., Pastor, V., …Holdsworth, M. J. (2019). The Arabidopsis thaliana N-recognin E3 ligase PROTEOLYSIS1 influences the immune response. Plant Direct, 3(12), https://doi.org/10.1002/pld3.194

Journal Article Type Article
Acceptance Date Dec 2, 2019
Online Publication Date Dec 26, 2019
Publication Date Dec 26, 2019
Deposit Date Dec 12, 2019
Publicly Available Date Dec 26, 2019
Journal Plant Direct
Electronic ISSN 2475-4455
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 3
Issue 12
Article Number e00194
DOI https://doi.org/10.1002/pld3.194
Public URL https://nottingham-repository.worktribe.com/output/3542249
Publisher URL https://onlinelibrary.wiley.com/doi/full/10.1002/pld3.194

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The Arabidopsis thaliana N‐recognin E3 ligase PROTEOLYSIS1 influences the immune response (3.8 Mb)
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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/





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