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The plant N‐degron pathways of ubiquitin‐mediated proteolysis

Holdsworth, Michael John; Vicente, Jorge; Sharma, Gunjan; Abbas, Mohamad; Zubrycka, Agata

Authors

Agata Zubrycka



Abstract

The amino‐terminal residue of a protein (or amino‐terminus of a peptide following protease cleavage) can be an important determinant of its stability, through the Ubiquitin‐Proteasome‐System associated N‐degron pathways. Plants contain a unique combination of N‐degron pathways (previously called the N‐end rule pathways) E3 ligases, PROTEOLYSIS (PRT)6 and PRT1, recognising non‐overlapping sets of amino‐terminal residues, and others remain to be identified. Although only very few substrates of PRT1 or PRT6 have been identified, substrates of the oxygen and nitric oxide sensing branch of the PRT6 N‐degron pathway include key nuclear‐located transcription factors (ETHYLENE RESPONSE FACTOR VIIs and LITTLE ZIPPER 2) and the histone‐modifying Polycomb Repressive Complex 2 component VERNALISATION 2. In response to reduced oxygen or nitric oxide levels (and other mechanisms that reduce pathway activity) these stabilised substrates regulate diverse aspects of growth and development, including response to flooding, salinity, vernalisation (cold‐induced flowering) and shoot apical meristem function. The N‐degron pathways show great promise for use in the improvement of crop performance and for biotechnological applications. Upstream proteases, components of the different pathways and associated substrates still remain to be identified and characterised to fully appreciate how regulation of protein stability through the amino‐terminal residue impacts plant biology.

Citation

Holdsworth, M. J., Vicente, J., Sharma, G., Abbas, M., & Zubrycka, A. (2020). The plant N‐degron pathways of ubiquitin‐mediated proteolysis. Journal of Integrative Plant Biology, 62(1), 70-89. https://doi.org/10.1111/jipb.12882

Journal Article Type Article
Acceptance Date Oct 18, 2019
Online Publication Date Oct 22, 2019
Publication Date 2020-01
Deposit Date Oct 28, 2019
Publicly Available Date Oct 23, 2020
Journal Journal of Integrative Plant Biology
Print ISSN 1672-9072
Electronic ISSN 1744-7909
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 62
Issue 1
Pages 70-89
DOI https://doi.org/10.1111/jipb.12882
Keywords Plant Science; General Biochemistry, Genetics and Molecular Biology; Biochemistry
Public URL https://nottingham-repository.worktribe.com/output/2975461
Publisher URL https://onlinelibrary.wiley.com/doi/abs/10.1111/jipb.12882
Additional Information This is the pre-peer reviewed version of the following article: Holdsworth, M. J., Vicente, J. , Sharma, G. , Abbas, M. and Zubrycka, A. (2019), The plant N‐degron pathways of ubiquitin‐mediated proteolysis. J. Integr. Plant Biol. Accepted Author Manuscript, which has been published in final form at https://doi.org/10.1111/jipb.12882. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

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