Unravelling transmission of Mycobacterium avium subspecies paratuberculosis to dairy calves: results of a lifelong longitudinal study

Nunney, Erica; Crotta, Matteo; van Winden, Steven; Bond, Karen; Green, Martin; Guitian, Javier

Abstract

Johne's disease (JD) is a chronic disease of ruminants endemic in the UK and other countries and responsible for large economic losses for the dairy sector. JD is caused by Mycobacterium avium subspecies paratuberculosis (MAP), which typically infects calves that remain latently infected during a long period, making early detection of infection challenging. Cow to calf transmission can occur in-utero, via milk/colostrum or faecal-orally. Understanding of the different transmission routes to calves is important in informing control recommendations. Our aim in this longitudinal study was to measure the association between the transmission routes via the dam and the environment on a calf subsequently testing serologically positive for MAP. The study population comprised of 439 UK dairy calves from 6 herds enrolled between 2012 and 2013. These calves were followed up from birth until 2023. At birth individual calf data was captured. During follow-up, individuals entering the milking herd were quarterly tested for the presence of MAP antibodies using milk ELISA. Cox regression models were used to measure the association between exposure from the dam (in-utero and/or colostrum) or from the environment (long time in dirty yard) and time to first detection of MAP infection. An association between calves born to positive dams and probability of having a MAP positive test result remained after excluding potential MAP transmission via colostrum (Hazard ratio: 2.24; 95% CI: 1.14 – 4.41). Calves unlikely to be infected with MAP via the in-utero or colostrum route, had 3.68 (95% CI: 3.68 1.45–9.33) higher hazard of a positive test result when they stayed longer in a dirty calving area. The effect of the dam infection status on transmission to calves precedes the dam's seroconversion and persists after excluding the potential role of transmission via colostrum. The association between time spent in a dirty calving area and probability of a MAP positive test result highlights the role of environmental contamination as a source of infection in addition to the dam.

Citation

Nunney, E., Crotta, M., van Winden, S., Bond, K., Green, M., & Guitian, J. (2023). Unravelling transmission of Mycobacterium avium subspecies paratuberculosis to dairy calves: results of a lifelong longitudinal study. Preventive Veterinary Medicine, 219, Article 106022. https://doi.org/10.1016/j.prevetmed.2023.106022

• Journal Article Type: Article
• Acceptance Date: Sep 5, 2023
• Online Publication Date: Sep 9, 2023
• Publication Date: 2023-10
• Deposit Date: Nov 21, 2023
• Publicly Available Date: Nov 21, 2023
• Journal: Preventive Veterinary Medicine
• Print ISSN: 0167-5877
• Electronic ISSN: 1873-1716
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 219
• Article Number: 106022
• DOI: https://doi.org/10.1016/j.prevetmed.2023.106022
• Keywords: Johne’s disease, Calves, Dairy cattle, Mycobacterium avium subspecies paratuberculosis, Milk ELISA, Transmission
• Public URL: https://nottingham-repository.worktribe.com/output/25953037
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0167587723001861?via%3Dihub