Dan Chen
The multitasking Fasciola gigantica Cathepsin B interferes with various functions of goat peripheral blood mononuclear cells in vitro
Chen, Dan; Tian, Ai-Ling; Hou, Jun-Ling; Li, Jie-Xi; Tian, XiaoWei; Yuan, Xiao-Dan; Li, Xiangrui; Elsheikha, Hany M.; Zhu, Xing-Quan
Authors
Ai-Ling Tian
Jun-Ling Hou
Jie-Xi Li
XiaoWei Tian
Xiao-Dan Yuan
Xiangrui Li
Professor HANY ELSHEIKHA hany.elsheikha@nottingham.ac.uk
Professor of Interdisciplinary Parasitology
Xing-Quan Zhu
Abstract
Cathepsin B, a lysosomal cysteine protease, is thought to be involved in the pathogenesis of Fasciola gigantica infection, but its exact role remains unclear. In the present study, a recombinant F. gigantica cathepsin B (rFgCatB) protein was expressed in the methylotrophic yeast Pichia pastoris. Western blot analysis confirmed the reactivity of the purified rFgCatB protein to serum from F. gigantica-infected goats. The effects of serial concentrations (10, 20, 40, 80, and 160 μg/ml) of rFgCatB on various functions of goat peripheral blood mononuclear cells (PBMCs) were examined. We demonstrated that rFgCatB protein can specifically bind to the surface of PBMCs. In addition, rFgCatB increased the expression of cytokines (IL-2, IL-4, IL-10, IL-17, TGF-β, and IFN-γ), and increased nitric oxide production and cell apoptosis, but reduced cell viability. These data show that rFgCatB can influence cellular and immunological functions of goat PBMCs. Further characterization of the posttranslational modification and assessment of rFgCatB in immunogenicity studies is warranted.
Citation
Chen, D., Tian, A., Hou, J., Li, J., Tian, X., Yuan, X., …Zhu, X. (2019). The multitasking Fasciola gigantica Cathepsin B interferes with various functions of goat peripheral blood mononuclear cells in vitro. Frontiers in Immunology, 10, Article 1707. https://doi.org/10.3389/fimmu.2019.01707
Journal Article Type | Article |
---|---|
Acceptance Date | Jul 8, 2019 |
Online Publication Date | Jul 23, 2019 |
Publication Date | Jul 23, 2019 |
Deposit Date | Jul 26, 2019 |
Publicly Available Date | Jul 26, 2019 |
Journal | Frontiers in Immunology |
Electronic ISSN | 1664-3224 |
Publisher | Frontiers Media |
Peer Reviewed | Peer Reviewed |
Volume | 10 |
Article Number | 1707 |
DOI | https://doi.org/10.3389/fimmu.2019.01707 |
Public URL | https://nottingham-repository.worktribe.com/output/2345495 |
Publisher URL | https://www.frontiersin.org/articles/10.3389/fimmu.2019.01707/full |
Additional Information | The Multitasking Fasciola gigantica Cathepsin B Interferes With Various Functions of Goat Peripheral Blood Mononuclear Cells in vitro, Dan Chen, Ai-Ling Tian, Jun-Ling Hou, Jie-Xi Li, XiaoWei Tian, Xiao-Dan Yuan, Xiangrui Li, Hany M. Elsheikha and Xing-Quan Zhu, Front. Immunol., v. 10, article 1707. |
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The Multitasking Fasciola gigantica Cathepsin B Interferes With Various Functions of Goat Peripheral Blood Mononuclear Cells in vitro
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