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The multitasking Fasciola gigantica Cathepsin B interferes with various functions of goat peripheral blood mononuclear cells in vitro

Chen, Dan; Tian, Ai-Ling; Hou, Jun-Ling; Li, Jie-Xi; Tian, XiaoWei; Yuan, Xiao-Dan; Li, Xiangrui; Elsheikha, Hany M.; Zhu, Xing-Quan

Authors

Dan Chen

Ai-Ling Tian

Jun-Ling Hou

Jie-Xi Li

XiaoWei Tian

Xiao-Dan Yuan

Xiangrui Li

Xing-Quan Zhu



Abstract

Cathepsin B, a lysosomal cysteine protease, is thought to be involved in the pathogenesis of Fasciola gigantica infection, but its exact role remains unclear. In the present study, a recombinant F. gigantica cathepsin B (rFgCatB) protein was expressed in the methylotrophic yeast Pichia pastoris. Western blot analysis confirmed the reactivity of the purified rFgCatB protein to serum from F. gigantica-infected goats. The effects of serial concentrations (10, 20, 40, 80, and 160 μg/ml) of rFgCatB on various functions of goat peripheral blood mononuclear cells (PBMCs) were examined. We demonstrated that rFgCatB protein can specifically bind to the surface of PBMCs. In addition, rFgCatB increased the expression of cytokines (IL-2, IL-4, IL-10, IL-17, TGF-β, and IFN-γ), and increased nitric oxide production and cell apoptosis, but reduced cell viability. These data show that rFgCatB can influence cellular and immunological functions of goat PBMCs. Further characterization of the posttranslational modification and assessment of rFgCatB in immunogenicity studies is warranted.

Citation

Chen, D., Tian, A., Hou, J., Li, J., Tian, X., Yuan, X., …Zhu, X. (2019). The multitasking Fasciola gigantica Cathepsin B interferes with various functions of goat peripheral blood mononuclear cells in vitro. Frontiers in Immunology, 10, Article 1707. https://doi.org/10.3389/fimmu.2019.01707

Journal Article Type Article
Acceptance Date Jul 8, 2019
Online Publication Date Jul 23, 2019
Publication Date Jul 23, 2019
Deposit Date Jul 26, 2019
Publicly Available Date Jul 26, 2019
Journal Frontiers in Immunology
Electronic ISSN 1664-3224
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 10
Article Number 1707
DOI https://doi.org/10.3389/fimmu.2019.01707
Public URL https://nottingham-repository.worktribe.com/output/2345495
Publisher URL https://www.frontiersin.org/articles/10.3389/fimmu.2019.01707/full
Additional Information The Multitasking Fasciola gigantica Cathepsin B Interferes With Various Functions of Goat Peripheral Blood Mononuclear Cells in vitro,
Dan Chen, Ai-Ling Tian, Jun-Ling Hou, Jie-Xi Li, XiaoWei Tian, Xiao-Dan Yuan, Xiangrui Li, Hany M. Elsheikha and Xing-Quan Zhu, Front. Immunol., v. 10, article 1707.

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