Skip to main content

Research Repository

Advanced Search

Identification of nitrogen-dependent QTL and underlying genes for root system architecture in hexaploid wheat

Griffiths, Marcus; Atkinson, Jonathan A.; Gardiner, Laura-Jayne; Swarup, Ranjan; Pound, Michael P.; Wilson, Michael H.; Bennett, Malcolm J.; Wells, Darren M.

Authors

Marcus Griffiths

JONATHAN ATKINSON JONATHAN.ATKINSON@NOTTINGHAM.AC.UK
Future Food Beacon:Technologist in Phenomics

Laura-Jayne Gardiner

MICHAEL WILSON MICHAEL.WILSON@NOTTINGHAM.AC.UK
Future Food Beacon:Technologist in Bioinformatics/Computational Biology

DARREN WELLS DARREN.WELLS@NOTTINGHAM.AC.UK
Principal Research Fellow



Abstract

The root system architecture (RSA) of a crop has a profound effect on the uptake of nutrients and consequently the potential yield. However, little is known about the genetic basis of RSA and resource dependent response in wheat (Triticum aestivum L.). Here, a high-throughput hydroponic root phenotyping system was used to identify N-dependent root traits in a wheat mapping population. Using quantitative trait locus (QTL) analysis, a total of 55 QTLs were discovered for seedling root traits across two N treatments, 25 of which were N-dependent. Transcriptomic analyses were used on a N-dependent root angle QTL on chromosome 2D and 17 candidate genes were identified. Of these N-dependent genes a nitrate transporter 1/peptide transporter (NPF) family gene was upregulated making it an interesting candidate for N signalling and response processes for root angle change. The RNA-seq results provide valuable genetic insight for root angle control, N-dependent responses and candidate genes for improvement of N capture in wheat.

Citation

Griffiths, M., Atkinson, J. A., Gardiner, L., Swarup, R., Pound, M. P., Wilson, M. H., …Wells, D. M. (2019). Identification of nitrogen-dependent QTL and underlying genes for root system architecture in hexaploid wheat

Other Type Other
Acceptance Date Mar 28, 2019
Online Publication Date Mar 28, 2019
Publication Date Mar 28, 2019
Deposit Date Apr 30, 2019
Publicly Available Date May 13, 2019
Publisher Cold Spring Harbor Laboratory Press
DOI https://doi.org/10.1101/591529
Public URL https://nottingham-repository.worktribe.com/output/1854424
Related Public URLs https://www.biorxiv.org/content/10.1101/591529v1

Files





You might also like



Downloadable Citations