Skip to main content

Research Repository

Advanced Search

Magnetically Assisted Control of Stem Cells Applied in 2D, 3D and In Situ Models of Cell Migration

Harrison, Richard; Luckett, Jeni; Marsh, Sarah; Lugo Leija, Hilda Anaid; Salih, Shelanah; Alkharji, Reem; Sottile, Virginie

Authors

Richard Harrison

Sarah Marsh

Hilda Anaid Lugo Leija

Shelanah Salih

Reem Alkharji

Virginie Sottile



Abstract

The success of cell therapy approaches is greatly dependent on the ability to precisely deliver and monitor transplanted stem cell grafts at treated sites. Iron oxide particles, traditionally used in vivo for magnetic resonance imaging (MRI), have been shown to also represent a safe and efficient in vitro labelling agent for mesenchymal stem cells (MSCs). Here, stem cells were labelled with magnetic particles, and their resulting response to magnetic forces was studied using 2D and 3D models. Labelled cells exhibited magnetic responsiveness, which promoted localised retention and patterned cell seeding when exposed to magnet arrangements in vitro. Directed migration was observed in 2D culture when adherent cells were exposed to a magnetic field, and also when cells were seeded into a 3D gel. Finally, a model of cell injection into the rodent leg was used to test the enhanced localised retention of labelled stem cells when applying magnetic forces, using whole body imaging to confirm the potential use of magnetic particles in strategies seeking to better control cell distribution for in vivo cell delivery.

Journal Article Type Article
Publication Date Apr 2, 2019
Journal Molecules
Electronic ISSN 1420-3049
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 24
Issue 8
Article Number 1563
APA6 Citation Harrison, R., Luckett, J., Marsh, S., Lugo Leija, H. A., Salih, S., Alkharji, R., & Sottile, V. (2019). Magnetically Assisted Control of Stem Cells Applied in 2D, 3D and In Situ Models of Cell Migration. Molecules, 24(8), https://doi.org/10.3390/molecules24081563
DOI https://doi.org/10.3390/molecules24081563
Keywords Organic Chemistry
Publisher URL https://www.mdpi.com/1420-3049/24/8/1563

Files





You might also like



Downloadable Citations

;