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TGF?1-induced cell motility but not cell proliferation is mediated through Cten in colorectal cancer

Asiri, Abdulaziz; Raposo, Teresa Pereira; Alfahed, Abdulaziz; Ilyas, Mohammad


Abdulaziz Asiri

Teresa Pereira Raposo

Abdulaziz Alfahed


Cten (C-terminal tensin-like) is a member of the tensin protein family found in complex with integrins at focal adhesions. It promotes epithelial?mesenchymal transition (EMT) and cell motility. The precise mechanisms regulating Cten are unknown, although we and others have shown that Cten could be under the regulation of several cytokines and growth factors. Since Transforming growth factor beta 1 (TGF-?1) regulates integrin function and promotes EMT / cell motility, we were prompted to investigate whether TGF-?1 induces EMT and cell motility through Cten signalling in colorectal cancer.

TGF-?1 signalling was modulated by either stimulation with TGF-?1 or knockdown of TGF-?1 in the CRC cell lines SW620 and HCT116. The effect of this modulation on expression of Cten, EMT markers and on cellular function was tested. The role of Cten as a direct mediator of TGF-?1 signalling was investigated in a CRC cell line in which the Cten gene had been deleted (SW620?Cten).

When TGF-?1 was stimulated or inhibited, this resulted in, respectively, upregulation and downregulation of Cten expression and EMT markers (Snail, Rock, N-Cadherin, Src). Cell migration and cell invasion were significantly increased following TGF-?1 stimulation and lost by TGF-?1 knockdown. TGF-?1 stimulation of the SW620?Cten cell line resulted in selective loss of the effect of TGF-?1 signalling pathway on EMT and cell motility whilst the stimulatory effect on cell proliferation was retained.

These data suggested Cten may play an essential role in mediating TGF-?1 induced EMT and cell motility and may therefore play a role in metastasis in CRC.


Asiri, A., Raposo, T. P., Alfahed, A., & Ilyas, M. (2018). TGF?1-induced cell motility but not cell proliferation is mediated through Cten in colorectal cancer. International Journal of Experimental Pathology, 99(6), 323-330.

Journal Article Type Article
Acceptance Date Dec 6, 2018
Online Publication Date Jan 15, 2019
Publication Date Dec 1, 2018
Deposit Date Jan 24, 2019
Publicly Available Date Dec 2, 2019
Journal International Journal of Experimental Pathology
Print ISSN 0959-9673
Electronic ISSN 1365-2613
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 99
Issue 6
Pages 323-330
Keywords Epithelial?mesenchymal transition (EMT); Colorectal cancer; Transforming growth factor beta (TGF??); Cell motility; Cell invasion
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