Outputs (165)

Kinetic analysis of fluorescent ligand binding to cell surface receptors: Insights into conformational changes and allosterism in living cells (2023)
Journal Article
Hill, S. J., & Kilpatrick, L. E. (2023). Kinetic analysis of fluorescent ligand binding to cell surface receptors: Insights into conformational changes and allosterism in living cells. British Journal of Pharmacology, https://doi.org/10.1111/bph.16185

Equilibrium binding assays are one of the mainstays of current drug discovery efforts to evaluate the interaction of drugs with receptors in membranes and intact cells. However, in recent years, there has been increased focus on the kinetics of the d... Read More about Kinetic analysis of fluorescent ligand binding to cell surface receptors: Insights into conformational changes and allosterism in living cells.

Escherichia coli DNA repair helicase Lhr is also a uracil‐DNA glycosylase (2023)
Journal Article
Buckley, R. J., Lou‐Hing, A., Hanson, K. M., Ahmed, N. R., Cooper, C. D. O., & Bolt, E. L. (2023). Escherichia coli DNA repair helicase Lhr is also a uracil‐DNA glycosylase. Molecular Microbiology, 120(2), 298-306. https://doi.org/10.1111/mmi.15123

DNA glycosylases protect genetic fidelity during DNA replication by removing potentially mutagenic chemically damaged DNA bases. Bacterial Lhr proteins are well‐characterized DNA repair helicases that are fused to additional 600–700 amino acids of un... Read More about Escherichia coli DNA repair helicase Lhr is also a uracil‐DNA glycosylase.

The Lipid Phosphate Phosphatase Wunen Promotes Eggshell Formation and Is Essential for Fertility in Drosophila (2023)
Journal Article
Mukherjee, A., Schuppe, M., & Renault, A. D. (2023). The Lipid Phosphate Phosphatase Wunen Promotes Eggshell Formation and Is Essential for Fertility in Drosophila. Biology, 12(7), Article 1003. https://doi.org/10.3390/biology12071003

The eggshell that surrounds insect eggs acts as a barrier, protecting against biotic factors and desiccation. The eggshell is a multi-layered structure which is synthesised by the somatic follicle cells that surround the developing oocyte. Although t... Read More about The Lipid Phosphate Phosphatase Wunen Promotes Eggshell Formation and Is Essential for Fertility in Drosophila.

Escherichia coli DNA repair helicase Lhr is also a uracil-DNA glycosylase (2023)
Journal Article
Buckley, R. J., Lou-Hing, A., Hanson, K. M., Ahmed, N. R., Cooper, C. D., & Bolt, E. L. (2023). Escherichia coli DNA repair helicase Lhr is also a uracil-DNA glycosylase. Molecular Microbiology, 120(2), 298-306. https://doi.org/10.1111/mmi.15123

DNA glycosylases protect genetic fidelity during DNA replication by removing potentially mutagenic chemically damaged DNA bases. Bacterial Lhr proteins are well-characterized DNA repair helicases that are fused to additional 600–700 amino acids of un... Read More about Escherichia coli DNA repair helicase Lhr is also a uracil-DNA glycosylase.

A new species of the genus Anamylopsora (Baeomycetaceae; Ascomycota) from Deosai National Park, Gilgit-Baltistan, Pakistan (2023)
Journal Article
Usman, M., Firdous, Q., Dyer, P. S., & Khalid, A. N. (2023). A new species of the genus Anamylopsora (Baeomycetaceae; Ascomycota) from Deosai National Park, Gilgit-Baltistan, Pakistan. Lichenologist, https://doi.org/10.1017/s002428292300018x

A novel lichen species occurring on rocks was collected from three different localities within Deosai National Park, Gilgit-Baltistan, Pakistan. Phylogenetic analyses of the nrDNA ITS and nuLSU regions revealed that it clustered within the genus Anam... Read More about A new species of the genus Anamylopsora (Baeomycetaceae; Ascomycota) from Deosai National Park, Gilgit-Baltistan, Pakistan.

Cellular Responses and Targets in Food Spoilage Yeasts Exposed to Antifungal Prenylated Isoflavonoids (2023)
Journal Article
Kalli, S., Vallieres, C., Violet, J., Sanders, J.-W., Chapman, J., Vincken, J.-P., …Araya-Cloutier, C. (2023). Cellular Responses and Targets in Food Spoilage Yeasts Exposed to Antifungal Prenylated Isoflavonoids. Microbiology Spectrum, 11(4), Article e0132723. https://doi.org/10.1128/spectrum.01327-23

Prenylated isoflavonoids are phytochemicals with promising antifungal properties. Recently, it was shown that glabridin and wighteone disrupted the plasma membrane (PM) of the food spoilage yeast Zygosaccharomyces parabailii in distinct ways, which l... Read More about Cellular Responses and Targets in Food Spoilage Yeasts Exposed to Antifungal Prenylated Isoflavonoids.

Associations of obesity phenotypes with weight change, cardiometabolic benefits, and type 2 diabetes incidence during a lifestyle intervention: results from the PREVIEW study (2023)
Journal Article
Zhu, R., Huttunen-Lenz, M., Stratton, G., Handjieva-Darlenska, T., Handjiev, S., Sundvall, J., …Raben, A. (2023). Associations of obesity phenotypes with weight change, cardiometabolic benefits, and type 2 diabetes incidence during a lifestyle intervention: results from the PREVIEW study. International Journal of Obesity, 47(9), 833–840. https://doi.org/10.1038/s41366-023-01328-y

Background/Objectives Some individuals with overweight/obesity may be relatively metabolically healthy (MHO) and have a lower risk of cardiovascular disease than those with metabolically unhealthy overweight/obesity (MUO). We aimed to compare change... Read More about Associations of obesity phenotypes with weight change, cardiometabolic benefits, and type 2 diabetes incidence during a lifestyle intervention: results from the PREVIEW study.

Archaeal Hel308 suppresses recombination through a catalytic switch that controls DNA annealing (2023)
Journal Article
Lever, R., Simmons, E., Gamble-Milner, R., Buckley, R., Harrison, C., Parkes, A., …Allers, T. (2023). Archaeal Hel308 suppresses recombination through a catalytic switch that controls DNA annealing. Nucleic Acids Research, 51(16), 8563-8574. https://doi.org/10.1093/nar/gkad572

Hel308 helicases promote genome stability in archaea and are conserved in metazoans, where they are known as HELQ. Their helicase mechanism is well characterised, but it is unclear how they specifically contribute to genome stability in archaea. We s... Read More about Archaeal Hel308 suppresses recombination through a catalytic switch that controls DNA annealing.

Characterisation of tyrosine kinase inhibitor-receptor interactions at VEGFR2 using sunitinib-red and nanoBRET (2023)
Journal Article
Van Daele, M., Kilpatrick, L. E., Woolard, J., & Hill, S. J. (2023). Characterisation of tyrosine kinase inhibitor-receptor interactions at VEGFR2 using sunitinib-red and nanoBRET. Biochemical Pharmacology, 214, Article 115672. https://doi.org/10.1016/j.bcp.2023.115672

Vascular endothelial growth factor (VEGF) is an important mediator of angiogenesis, proliferation and migration of vascular endothelial cells. It is well known that cardiovascular safety liability for a wide range of small molecule tyrosine kinase in... Read More about Characterisation of tyrosine kinase inhibitor-receptor interactions at VEGFR2 using sunitinib-red and nanoBRET.

Probing expression of E-selectin using CRISPR-Cas9-mediated tagging with HiBiT in human endothelial cells (2023)
Journal Article
Ogrodzinski, L., Platt, S., Goulding, J., Alexander, C., Farr, T. D., Woolard, J., …Kilpatrick, L. E. (2023). Probing expression of E-selectin using CRISPR-Cas9-mediated tagging with HiBiT in human endothelial cells. iScience, 26(7), Article 107232. https://doi.org/10.1016/j.isci.2023.107232

E-selectin is expressed on endothelial cells in response to inflammatory cytokines and mediates leukocyte rolling and extravasation. However, studies have been hampered by lack of experimental approaches to monitor expression in real time in living c... Read More about Probing expression of E-selectin using CRISPR-Cas9-mediated tagging with HiBiT in human endothelial cells.