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Dr LAURA SIDNEY's Outputs (26)

Low-temperature vacuum evaporation as a novel dehydration process for the long-term preservation of transplantable human corneal tissue (2025)
Journal Article
McIntosh, O. D., Britchford, E. R., Beeken, L. J., Hopkinson, A., & Sidney, L. E. (2025). Low-temperature vacuum evaporation as a novel dehydration process for the long-term preservation of transplantable human corneal tissue. Cell and Tissue Banking, 26(1), Article 7. https://doi.org/10.1007/s10561-024-10155-y

Globally there is a shortage of available donor corneas with only 1 cornea available for every 70 needed. A large limitation to corneal transplant surgery is access to quality donor tissue due to inadequate eye donation services and infrastructure in... Read More about Low-temperature vacuum evaporation as a novel dehydration process for the long-term preservation of transplantable human corneal tissue.

Comparative cytotoxicity of Acanthamoeba castellanii-derived conditioned medium on human corneal epithelial and stromal cells (2024)
Journal Article
Alhazmi, A., Sidney, L. E., Hopkinson, A., & Elsheikha, H. M. (2024). Comparative cytotoxicity of Acanthamoeba castellanii-derived conditioned medium on human corneal epithelial and stromal cells. Acta Tropica, 257, Article 107288. https://doi.org/10.1016/j.actatropica.2024.107288

Soluble factors in the secretome of Acanthamoeba castellanii play crucial roles in the pathogenesis of Acanthamoeba keratitis (AK). Investigating the pathological effects of A. castellanii-derived conditioned medium (ACCM) on ocular cells can provide... Read More about Comparative cytotoxicity of Acanthamoeba castellanii-derived conditioned medium on human corneal epithelial and stromal cells.

Increased Anti-Inflammatory Therapeutic Potential and Progenitor Marker Expression of Corneal Mesenchymal Stem Cells Cultured in an Optimized Propagation Medium (2024)
Journal Article
Hopkinson, A., Notara, M., Cursiefen, C., & Sidney, L. E. (2024). Increased Anti-Inflammatory Therapeutic Potential and Progenitor Marker Expression of Corneal Mesenchymal Stem Cells Cultured in an Optimized Propagation Medium. Cell Transplantation, 33, 1-17. https://doi.org/10.1177/09636897241241992

There is a huge unmet need for new treatment modalities for ocular surface inflammatory disorders (OSIDs) such as dry eye disease and meibomian gland dysfunction. Mesenchymal stem cell therapies may hold the answer due to their potent immunomodulator... Read More about Increased Anti-Inflammatory Therapeutic Potential and Progenitor Marker Expression of Corneal Mesenchymal Stem Cells Cultured in an Optimized Propagation Medium.

Potential of mesenchymal stem cells as topical immunomodulatory cell therapies for ocular surface inflammatory disorders (2020)
Journal Article
Beeken, L. J., Ting, D. S. J., & Sidney, L. E. (2020). Potential of mesenchymal stem cells as topical immunomodulatory cell therapies for ocular surface inflammatory disorders. Stem Cells Translational Medicine, 10(1), 39-49. https://doi.org/10.1002/sctm.20-0118

Ocular surface inflammatory disorders (OSIDs) are a group of highly prevalent, heterogeneous diseases which display a variety of aetiologies and symptoms and are risk factors for serious complications including ocular and cornea impairment. Corneal i... Read More about Potential of mesenchymal stem cells as topical immunomodulatory cell therapies for ocular surface inflammatory disorders.

Preparation of Dried Amniotic Membrane for Corneal Repair (2020)
Book Chapter
Hopkinson, A., Britchford, E. R., & Sidney, L. E. (2020). Preparation of Dried Amniotic Membrane for Corneal Repair. In M. Ahearne (Ed.), Corneal Regeneration: Methods and Protocols (143-157). Springer US. https://doi.org/10.1007/978-1-0716-0599-8_10

Amniotic membrane transplantation is an established therapeutic and biological adjunct for several clinical situations, including treatment of diabetic foot ulcers and ocular surface disease. However, poorly standardized and validated clinical prepar... Read More about Preparation of Dried Amniotic Membrane for Corneal Repair.

Bioinspired Precision Engineering of Three-Dimensional Epithelial Stem Cell Microniches (2020)
Journal Article
Prina, E., Amer, M. H., Sidney, L., Tromayer, M., Moore, J., Liska, R., Bertolin, M., Ferrari, S., Hopkinson, A., Dua, H., Yang, J., Wildman, R., & Rose, F. R. (2020). Bioinspired Precision Engineering of Three-Dimensional Epithelial Stem Cell Microniches. Advanced Biosystems, 4(6), Article 2000016. https://doi.org/10.1002/adbi.202000016

© 2020 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Maintenance of the epithelium relies on stem cells residing within specialized microenvironments, known as epithelial crypts. Two-photon polymerization (2PP) is a valuable... Read More about Bioinspired Precision Engineering of Three-Dimensional Epithelial Stem Cell Microniches.

The electrospinning of a thermo-responsive polymer with peptide conjugates for phenotype support and extracellular matrix production of therapeutically relevant mammalian cells (2020)
Journal Article
Ruiter, F. A., Sidney, L. E., Kiick, K. L., Segal, J. I., Alexander, C., & Rose, F. R. A. J. (2020). The electrospinning of a thermo-responsive polymer with peptide conjugates for phenotype support and extracellular matrix production of therapeutically relevant mammalian cells. Biomaterials Science, 8(9), 2611-2626. https://doi.org/10.1039/c9bm01965k

Current cell expansion methods for tissue engineering and regenerative medicine applications rely on the use of enzymatic digestion passaging and 2D platforms. However, this enzymatic treatment significantly reduces cell quality, due to the destructi... Read More about The electrospinning of a thermo-responsive polymer with peptide conjugates for phenotype support and extracellular matrix production of therapeutically relevant mammalian cells.

Validation and assessment of an antibiotic-based, aseptic decontamination manufacturing protocol for therapeutic, vacuum-dried human amniotic membrane (2019)
Journal Article
Marsit, N. M., Sidney, L. E., Britchford, E. R., McIntosh, O. D., Allen, C. L., Ashraf, W., Bayston, R., & Hopkinson, A. (2019). Validation and assessment of an antibiotic-based, aseptic decontamination manufacturing protocol for therapeutic, vacuum-dried human amniotic membrane. Scientific Reports, 9(1), Article 12854. https://doi.org/10.1038/s41598-019-49314-7

© 2019, The Author(s). Amniotic membrane (AM) is used to treat a range of ophthalmic indications but must be presented in a non-contaminated state. AM from elective caesarean sections contains natural microbial contamination, requiring removal during... Read More about Validation and assessment of an antibiotic-based, aseptic decontamination manufacturing protocol for therapeutic, vacuum-dried human amniotic membrane.

Anti-inflammatory potential of human corneal stroma-derived stem cells determined by a novel in vitro corneal epithelial injury model (2019)
Journal Article
Morales, M. L. O., Marsit, N. M., McIntosh, O. D., Hopkinson, A., & Sidney, L. E. (2019). Anti-inflammatory potential of human corneal stroma-derived stem cells determined by a novel in vitro corneal epithelial injury model. World Journal of Stem Cells, 11(2), 84-99. https://doi.org/10.4252/wjsc.v11.i2.84

BACKGROUND
An in vitro injury model mimicking a corneal surface injury was optimised using human corneal epithelial cells (hCEC).

AIM
To investigate whether corneal-stroma derived stem cells (CSSC) seeded on an amniotic membrane (AM) construct m... Read More about Anti-inflammatory potential of human corneal stroma-derived stem cells determined by a novel in vitro corneal epithelial injury model.

A thermoresponsive three-dimensional fibrous cell culture platform for enzyme-free expansion of mammalian cells (2019)
Journal Article
Aladdad, A. M., Amer, M. H., Sidney, L., Hopkinson, A., White, L. J., Alexander, C., & Rose, F. R. (2019). A thermoresponsive three-dimensional fibrous cell culture platform for enzyme-free expansion of mammalian cells. Acta Biomaterialia, 95, 427-438. https://doi.org/10.1016/j.actbio.2019.01.037

A three-dimensional thermoresponsive fibrous scaffold system for the subsequent extended culture and enzyme-free passaging of a range of mammalian cell types is presented. Poly(PEGMA188) was incorporated with poly(ethylene terephthalate) (PET) via bl... Read More about A thermoresponsive three-dimensional fibrous cell culture platform for enzyme-free expansion of mammalian cells.

In vitro evaluation of electrospun blends of gelatin and PCL for application as a partial thickness corneal graft (2018)
Journal Article
Rose, J., Sidney, L., Patient, J., White, L., Dua, H., El Haj, A., Hopkinson, A., & Rose, F. (2018). In vitro evaluation of electrospun blends of gelatin and PCL for application as a partial thickness corneal graft. Journal of Biomedical Materials Research Part A, 107(4), 828-838. https://doi.org/10.1002/jbm.a.36598

The advent of innovative surgical procedures utilizing partial thickness corneal grafts has created a need for the development of synthetic implants to recreate corneal stromal tissue. This work evaluates electrospun gelatin and polycaprolactone (PCL... Read More about In vitro evaluation of electrospun blends of gelatin and PCL for application as a partial thickness corneal graft.

Geometry alone influences stem cell differentiation in a precision 3D printed stem cell niche (2018)
Preprint / Working Paper
Prina, E., Sidney, L., Tromayer, M., Moore, J., Liska, R., Bertolin, M., Ferrari, S., Hopkinson, A., Dua, H., Yang, J., Wildman, R., & Rose, F. R. Geometry alone influences stem cell differentiation in a precision 3D printed stem cell niche

Stem cells within epithelial tissues reside in anatomical structures known as crypts that are known to contribute to the mechanical and chemical milieu important for function. To date, epithelial stem cell therapies have largely ignored the niche and... Read More about Geometry alone influences stem cell differentiation in a precision 3D printed stem cell niche.

3D microfabricated scaffolds and microfluidic devices for ocular surface replacement: a review (2017)
Journal Article
Prina, E., Mistry, P., Sidney, L. E., Yang, J., Wildman, R. D., Bertolin, M., Breda, C., Ferrari, B., Barbaro, V., Hopkinson, A., Dua, H. S., Ferrari, S., & Rose, F. R. A. (2017). 3D microfabricated scaffolds and microfluidic devices for ocular surface replacement: a review. Stem Cell Reviews and Reports, 13(3), 430-441. https://doi.org/10.1007/s12015-017-9740-6

In recent years, there has been increased research interest in generating corneal substitutes, either for use in the clinic or as in vitro corneal models. The advancement of 3D microfabrication technologies has allowed the reconstruction of the nativ... Read More about 3D microfabricated scaffolds and microfluidic devices for ocular surface replacement: a review.

Cultivation and characterisation of the surface markers and carbohydrate profile of human corneal endothelial cells (2016)
Journal Article
Nagymihály, R., Veréb, Z., Albert, R., Sidney, L., Dua, H., Hopkinson, A., & Petrovski, G. (2017). Cultivation and characterisation of the surface markers and carbohydrate profile of human corneal endothelial cells. Clinical and Experimental Ophthalmology, 45(5), 509-519. https://doi.org/10.1111/ceo.12903

Background
The study aims to characterise human corneal endothelial cell (HCEnC) cultures generated by the peel?and?digest method based on their surface protein/carbohydrate expression pattern.

Methods
Quantitative polymerase chain reaction was... Read More about Cultivation and characterisation of the surface markers and carbohydrate profile of human corneal endothelial cells.

Terminal sterilization: conventional methods versus emerging cold atmospheric pressure plasma technology for non-viable biological tissues (2016)
Journal Article
Marsit, N. M., Sidney, L. E., Branch, M. J., Wilson, S. L., & Hopkinson, A. (in press). Terminal sterilization: conventional methods versus emerging cold atmospheric pressure plasma technology for non-viable biological tissues. Plasma Processes and Polymers, https://doi.org/10.1002/ppap.201600134

Tissue products are susceptible to microbial contamination from different sources, which may cause disease transmission upon transplantation. Terminal sterilization using gamma radiation, electron-beam, and ethylene oxide protocols are well-establish... Read More about Terminal sterilization: conventional methods versus emerging cold atmospheric pressure plasma technology for non-viable biological tissues.

Corneal keratocyte transition to mesenchymal stem cell phenotype and reversal using serum-free medium supplemented with FGF-2, TGF-ß3 and retinoic acid (2016)
Journal Article
Sidney, L. E., & Hopkinson, A. (in press). Corneal keratocyte transition to mesenchymal stem cell phenotype and reversal using serum-free medium supplemented with FGF-2, TGF-ß3 and retinoic acid. Journal of Tissue Engineering and Regenerative Medicine, https://doi.org/10.1002/term.2316

Keratocytes of the corneal limbal stroma can derive populations of mesenchymal stem cells (MSC) when expanded in vitro. However, once a corneal MSC (cMSC) phenotype is achieved, regaining the keratocyte phenotype can be challenging, and there is no s... Read More about Corneal keratocyte transition to mesenchymal stem cell phenotype and reversal using serum-free medium supplemented with FGF-2, TGF-ß3 and retinoic acid.

Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells (2015)
Journal Article
Sidney, L. E., Branch, M. J., Dua, H. S., & Hopkinson, A. (2015). Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells. Cytotherapy, 17(12), https://doi.org/10.1016/j.jcyt.2015.08.003

Background: The limbal area of the corneal stroma has been identified as a source of mesenchymal-like stem cells, which have potential for exploitation as a cell therapy. However, the optimal culture conditions are disputed and few direct media compa... Read More about Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells.

Phenotypic change and induction of cytokeratin expression during in vitro culture of corneal stromal cells (2015)
Journal Article
Sidney, L. E., McIntosh, O. D., & Hopkinson, A. (2015). Phenotypic change and induction of cytokeratin expression during in vitro culture of corneal stromal cells. Investigative Ophthalmology & Visual Science, 56(12), https://doi.org/10.1167/iovs.15-17810

Purpose: Cells of the corneal epithelium and stroma can be distinguished in vivo by different intermediate filaments, cytokeratins for corneal epithelial cells (CEC) and vimentin for keratocytes. Isolated and cultured keratocytes change phenotype, lo... Read More about Phenotypic change and induction of cytokeratin expression during in vitro culture of corneal stromal cells.

Corneal Decellularization: A Method of Recycling Unsuitable Donor Tissue for Clinical Translation? (2015)
Journal Article
Wilson, S. L., Sidney, L. E., Dunphy, S. E., Dua, H. S., & Hopkinson, A. (2016). Corneal Decellularization: A Method of Recycling Unsuitable Donor Tissue for Clinical Translation?. Current Eye Research, 41(6), 769-782. https://doi.org/10.3109/02713683.2015.1062114

Background: There is a clinical need for biomimetic corneas that are as effective, preferably superior, to cadaveric donor tissue. Decellularized tissues are advantageous compared to synthetic or semi-synthetic engineered tissues in that the native m... Read More about Corneal Decellularization: A Method of Recycling Unsuitable Donor Tissue for Clinical Translation?.