Dr DAVID ONION's Outputs (16)

Lower Humoral and Cellular Immunity Following Asymptomatic SARS-CoV-2 Infection Compared to Symptomatic Infection in Education (The ACE Cohort) (2024)
Journal Article
Hopkins, G., Gomez, N., Tucis, D., Bartlett, L., Steers, G., Burns, E., Brown, M., Harvey-Cowlishaw, T., Santos, R., Lauder, S. N., Scurr, M., Capitani, L., Burnell, S., Rees, T., Smart, K., Somerville, M., Gallimore, A., Perera, M., Potts, M., Metaxaki, M., …Fairclough, L. (2024). Lower Humoral and Cellular Immunity Following Asymptomatic SARS-CoV-2 Infection Compared to Symptomatic Infection in Education (The ACE Cohort). Journal of Clinical Immunology, 44(6), Article 147. https://doi.org/10.1007/s10875-024-01739-0

Purpose
Asymptomatic SARS-CoV-2 infections were widely reported during the COVID-19 pandemic, acting as a hidden source of infection. Many existing studies investigating asymptomatic immunity failed to recruit true asymptomatic individuals. Thus, we... Read More about Lower Humoral and Cellular Immunity Following Asymptomatic SARS-CoV-2 Infection Compared to Symptomatic Infection in Education (The ACE Cohort).

The Role of Extracellular Vesicles in Allergic Sensitization: A Systematic Review (2024)
Journal Article
Tucis, D., Hopkins, G., Browne, W., James, V., Onion, D., & Fairclough, L. C. (2024). The Role of Extracellular Vesicles in Allergic Sensitization: A Systematic Review. International Journal of Molecular Sciences, 25(8), Article 4492. https://doi.org/10.3390/ijms25084492

Allergies affect approximately 10–30% of people worldwide, with an increasing number of cases each year; however, the underlying mechanisms are still poorly understood. In recent years, extracellular vesicles (EVs) have been suggested to play a role... Read More about The Role of Extracellular Vesicles in Allergic Sensitization: A Systematic Review.

PBMC-derived extracellular vesicles in a smoking-related inflammatory disease model (2023)
Journal Article
Gomez, N. E., James, V., Arkill, K. P., Nizamudeen, Z. A., Onion, D., & Fairclough, L. C. (2023). PBMC-derived extracellular vesicles in a smoking-related inflammatory disease model. European Journal of Immunology, 53(6), Article 2250143. https://doi.org/10.1002/eji.202250143

Extracellular vesicles (EVs) function as mediators of intercellular communication and as such influence the recipient cell function. EVs derived from immune cells can carry out many of the same functions as their parental cells, as they carry costimu... Read More about PBMC-derived extracellular vesicles in a smoking-related inflammatory disease model.

A mechanoresponsive nano-sized carrier achieves intracellular release of drug on external ultrasound stimulus (2022)
Journal Article
Catania, R., Onion, D., Russo, E., Zelzer, M., Mantovani, G., Huett, A., & Stolnik, S. (2022). A mechanoresponsive nano-sized carrier achieves intracellular release of drug on external ultrasound stimulus. RSC Advances, 12(26), 16561-16569. https://doi.org/10.1039/d2ra02307e

Control over intracellular release of therapeutic compounds incorporated into nano-carriers will open new possibilities for targeted treatments of various diseases including cancer, and viral and bacterial infections. Here we report our study on mech... Read More about A mechanoresponsive nano-sized carrier achieves intracellular release of drug on external ultrasound stimulus.

The Role of Lipids in Allergic Sensitization: A Systematic Review (2022)
Journal Article
Hopkins, G. V., Cochrane, S., Onion, D., & Fairclough, L. C. (2022). The Role of Lipids in Allergic Sensitization: A Systematic Review. Frontiers in Molecular Biosciences, 9, Article 832330. https://doi.org/10.3389/fmolb.2022.832330

Background: Immunoglobulin E (IgE)-mediated allergies are increasing in prevalence, with IgE-mediated food allergies currently affecting up to 10% of children and 6% of adults worldwide. The mechanisms underpinning the first phase of IgE-mediated all... Read More about The Role of Lipids in Allergic Sensitization: A Systematic Review.

Extracellular vesicles and chronic obstructive pulmonary disease (COPD): a systematic review (2022)
Journal Article
Gomez, N., James, V., Onion, D., & Fairclough, L. C. (2022). Extracellular vesicles and chronic obstructive pulmonary disease (COPD): a systematic review. Respiratory Research, 23, Article 82. https://doi.org/10.1186/s12931-022-01984-0

Background: Chronic Obstructive Pulmonary Disease (COPD) is a common inflammatory disease of the airways characterized by irreversible airflow limitation, ranking the third highest cause of death worldwide. Extracellular vesicles (EVs) are important... Read More about Extracellular vesicles and chronic obstructive pulmonary disease (COPD): a systematic review.

Extracellular Vesicles and Chronic Obstructive Pulmonary Disease (COPD): A Systematic Review (2021)
Preprint / Working Paper
Gomez, N., James, V., Onion, D., & Fairclough, L. Extracellular Vesicles and Chronic Obstructive Pulmonary Disease (COPD): A Systematic Review

Background: Chronic Obstructive Pulmonary Disease (COPD) is a common inflammatory disease of the airways characterized by irreversible airflow limitation, ranking the third highest cause of death worldwide. Extracellular vesicles (EVs) are important... Read More about Extracellular Vesicles and Chronic Obstructive Pulmonary Disease (COPD): A Systematic Review.

ABCB1 inhibition provides a novel therapeutic target to block TWIST1-induced migration in medulloblastoma (2021)
Journal Article
Nasir, A., Cardall, A., Othman, R. T., Nicolaou, N., Lourdusamy, A., Linke, F., …Coyle, B. (2021). ABCB1 inhibition provides a novel therapeutic target to block TWIST1-induced migration in medulloblastoma. Neuro-Oncology Advances, 3(1), https://doi.org/10.1093/noajnl/vdab030

Background: Therapeutic intervention in metastatic medulloblastoma is dependent upon elucidating the underlying metastatic mechanism. We investigated whether an epithelialmesenchymal transition (EMT)-like pathway could drive medulloblastoma metastasi... Read More about ABCB1 inhibition provides a novel therapeutic target to block TWIST1-induced migration in medulloblastoma.

A 3D Heterotypic Breast Cancer Model Demonstrates a Role for Mesenchymal Stem Cells in Driving a Proliferative and Invasive Phenotype (2020)
Journal Article
Pal, A., Ashworth, J. C., Collier, P., Probert, C., Jones, S., Leza, E. P., …Grabowska, A. M. (2020). A 3D Heterotypic Breast Cancer Model Demonstrates a Role for Mesenchymal Stem Cells in Driving a Proliferative and Invasive Phenotype. Cancers, 12(8), Article 2290. https://doi.org/10.3390/cancers12082290

Metabolism based isolation of invasive glioblastoma cells with specific gene signatures and tumorigenic potential (2020)
Journal Article
Smith, S. J., Rowlinson, J., Estevez-Cebrero, M., Onion, D., Ritchie, A., Clarke, P., …Rahman, R. (2020). Metabolism based isolation of invasive glioblastoma cells with specific gene signatures and tumorigenic potential. Neuro-Oncology Advances, 2(1), Article vdaa087. https://doi.org/10.1093/noajnl/vdaa087

Background
Glioblastoma (GBM) is a highly aggressive brain tumor with rapid subclonal diversification, harboring molecular abnormalities that vary temporo-spatially, a contributor to therapy resistance. Fluorescence guided neurosurgical resection ut... Read More about Metabolism based isolation of invasive glioblastoma cells with specific gene signatures and tumorigenic potential.

Characterization of human FcεRIα chain expression and gene copy number in humanized rat basophilic leukaemia (RBL) reporter cell lines (2019)
Journal Article
Ali Ali, E., Kalli, M., Wan, D., Nakamura, R., Onion, D., Alanine, D. G., …Falcone, F. H. (2019). Characterization of human FcεRIα chain expression and gene copy number in humanized rat basophilic leukaemia (RBL) reporter cell lines. PLoS ONE, 14(8), Article e0221034. https://doi.org/10.1371/journal.+pone.0221034

Several laboratories have created rat basophil leukemia (RBL) cell lines stably transfected with the human high affinity IgE receptor (FcεRI H). More recently, humanized RBL cell lines saw the introduction of reporter genes such as luciferase (RS-ATL... Read More about Characterization of human FcεRIα chain expression and gene copy number in humanized rat basophilic leukaemia (RBL) reporter cell lines.

Intracellular processing of silica-coated superparamagnetic iron nanoparticles in human mesenchymal stem cells (2019)
Journal Article
Harrison, R. P., Chauhan, V. M., Onion, D., Aylott, J. W., & Sottile, V. (2019). Intracellular processing of silica-coated superparamagnetic iron nanoparticles in human mesenchymal stem cells. RSC Advances, 9(6), 3176-3184. https://doi.org/10.1039/C8RA09089K

Silica-coated Superparamagnetic Iron Nanoparticles (SiMAGs) are an exciting biomedical technology capable of targeted delivery of cell-based therapeutics and disease diagnosis. However, in order to realise their full clinical potential, their intrace... Read More about Intracellular processing of silica-coated superparamagnetic iron nanoparticles in human mesenchymal stem cells.

Multicomponent analysis of the tumour microenvironment reveals low CD8 T cell number, low stromal caveolin-1 and high tenascin-C and their combination as significant prognostic markers in non-small cell lung cancer (2017)
Journal Article
Onion, D., Isherwood, M., Shridhar, N., Xenophontos, M., Craze, M. L., Day, L. J., …Grabowska, A. M. (2018). Multicomponent analysis of the tumour microenvironment reveals low CD8 T cell number, low stromal caveolin-1 and high tenascin-C and their combination as significant prognostic markers in non-small cell lung cancer. Oncotarget, 9(2), 1760-1771. https://doi.org/10.18632/oncotarget.18880

The complex interplay of the tumour microenvironment (TME) and its role in disease progression and response to therapy is poorly understood. The majority of studies to date focus on individual components or molecules within the TME and so lack the po... Read More about Multicomponent analysis of the tumour microenvironment reveals low CD8 T cell number, low stromal caveolin-1 and high tenascin-C and their combination as significant prognostic markers in non-small cell lung cancer.

Individual patient oesophageal cancer 3D models for tailored treatment (2016)
Journal Article
Saunders, J. H., Onion, D., Collier, P., Dorrington, M. S., Argent, R. H., Clarke, P. A., …Grabowska, A. M. (2017). Individual patient oesophageal cancer 3D models for tailored treatment. Oncotarget, 8(15), 24224-24236. https://doi.org/10.18632/oncotarget.12500

Background: A model to predict chemotherapy response would provide a marked clinical benefit, enabling tailored treatment of oesophageal cancer, where less than half of patients respond to the routinely administered chemotherapy.
Methods: Cancer cel... Read More about Individual patient oesophageal cancer 3D models for tailored treatment.

3-dimensional patient-derived lung cancer assays reveal resistance to standards-of-care promoted by stromal cells but sensitivity to histone deacetylase inhibitors (2016)
Journal Article
Onion, D., Argent, R. H., Reece-Smith, A. M., Craze, M. L., Pineda, R. G., Clarke, P. A., …Grabowska, A. M. (2016). 3-dimensional patient-derived lung cancer assays reveal resistance to standards-of-care promoted by stromal cells but sensitivity to histone deacetylase inhibitors. Molecular Cancer Therapeutics, 15(4), https://doi.org/10.1158/1535-7163.MCT-15-0598

There is a growing recognition that current preclinical models do not reflect the tumor microenvironment in cellular, biological, and biophysical content and this may have a profound effect on drug efficacy testing, especially in the era of molecular... Read More about 3-dimensional patient-derived lung cancer assays reveal resistance to standards-of-care promoted by stromal cells but sensitivity to histone deacetylase inhibitors.

Quantum dot labelling of adenovirus allows for highly sensitive single cell flow and imaging cytometry (2015)
Journal Article
Herod, M. R., Pineda, R. G., Mautner, V., & Onion, D. (2015). Quantum dot labelling of adenovirus allows for highly sensitive single cell flow and imaging cytometry. Small, 11(7), https://doi.org/10.1002/smll.201401885

A quantum dot method for highly efficient labelling of single adenoviral particles is developed. The technique has no impact on viral fitness and allows the imaging and tracking of virus binding and internalisation events using a variety of technique... Read More about Quantum dot labelling of adenovirus allows for highly sensitive single cell flow and imaging cytometry.