Outputs (20)

Atypical flagella assembly and haploid genome coiling during male gamete formation in Plasmodium (2023)
Journal Article
Hair, M., Moreira-Leite, F., Ferguson, D. J. P., Ferguson, D. J., Zeeshan, M., Tewari, R., & Vaughan, S. (2023). Atypical flagella assembly and haploid genome coiling during male gamete formation in Plasmodium. Nature Communications, 14(1), Article 8263. https://doi.org/10.1038/s41467-023-43877-w

Gametogenesis in Plasmodium spp. occurs within the Anopheles mosquito and is essential for sexual reproduction / differentiation and onwards transmission to mammalian hosts. To better understand the 3D organisation of male gametogenesis, we used seri... Read More about Atypical flagella assembly and haploid genome coiling during male gamete formation in Plasmodium.

Mechanochemical tuning of a kinesin motor essential for malaria parasite transmission (2022)
Journal Article
Liu, T., Shilliday, F., Cook, A. D., Zeeshan, M., Brady, D., Tewari, R., Sutherland, C. J., Roberts, A. J., & Moores, C. A. (2022). Mechanochemical tuning of a kinesin motor essential for malaria parasite transmission. Nature Communications, 13(1), Article 6988. https://doi.org/10.1038/s41467-022-34710-x

Plasmodium species cause malaria and kill hundreds of thousands annually. The microtubule-based motor kinesin-8B is required for development of the flagellated Plasmodium male gamete, and its absence completely blocks parasite transmission. To unders... Read More about Mechanochemical tuning of a kinesin motor essential for malaria parasite transmission.

Plasmodium SAS4: basal body component of male cell which is dispensable for parasite transmission (2022)
Journal Article
Zeeshan, M., Brady, D., Markus, R., Vaughan, S., Ferguson, D., Holder, A. A., & Tewari, R. (2022). Plasmodium SAS4: basal body component of male cell which is dispensable for parasite transmission. Life Science Alliance, 5(9), Article e202101329. https://doi.org/10.26508/lsa.202101329

The centriole/basal body (CBB) is an evolutionarily conserved organelle acting as a microtubule organising centre (MTOC) to nucleate cilia, flagella, and the centrosome. SAS4/CPAP is a conserved component associated with BB biogenesis in many model f... Read More about Plasmodium SAS4: basal body component of male cell which is dispensable for parasite transmission.

Cryo-EM structure of a microtubule-bound parasite kinesin motor and implications for its mechanism and inhibition (2021)
Journal Article
Cook, A. D., Roberts, A. J., Atherton, J., Tewari, R., Topf, M., & Moores, C. A. (2021). Cryo-EM structure of a microtubule-bound parasite kinesin motor and implications for its mechanism and inhibition. Journal of Biological Chemistry, 297(5), Article 101063. https://doi.org/10.1016/j.jbc.2021.101063

Plasmodium parasites cause malaria and are responsible annually for hundreds of thousands of deaths. Kinesins are a superfamily of microtubule-dependent ATPases that play important roles in the parasite replicative machinery, which is a potential tar... Read More about Cryo-EM structure of a microtubule-bound parasite kinesin motor and implications for its mechanism and inhibition.

Protein phosphatase 1 regulates atypical mitotic and meiotic division in Plasmodium sexual stages (2021)
Journal Article
Zeeshan, M., Pandey, R., Subudhi, A. K., Ferguson, D. J., Kaur, G., Rashpa, R., Nugmanova, R., Brady, D., Bottrill, A. R., Vaughan, S., Brochet, M., Bollen, M., Pain, A., Holder, A. A., Guttery, D. S., & Tewari, R. (2021). Protein phosphatase 1 regulates atypical mitotic and meiotic division in Plasmodium sexual stages. Communications Biology, 4(1), Article 760. https://doi.org/10.1038/s42003-021-02273-0

PP1 is a conserved eukaryotic serine/threonine phosphatase that regulates many aspects of mitosis and meiosis, often working in concert with other phosphatases, such as CDC14 and CDC25. The proliferative stages of the malaria parasite life cycle incl... Read More about Protein phosphatase 1 regulates atypical mitotic and meiotic division in Plasmodium sexual stages.

Molecular characterization of the conoid complex in Toxoplasma reveals its conservation in all apicomplexans, including Plasmodium species (2021)
Journal Article
Koreny, L., Zeeshan, M., Barylyuk, K., Tromer, E. C., van Hooff, J. J., Brady, D., Ke, H., Chelaghma, S., Ferguson, D. J., Eme, L., Tewari, R., & Waller, R. F. (2021). Molecular characterization of the conoid complex in Toxoplasma reveals its conservation in all apicomplexans, including Plasmodium species. PLoS Biology, 19(3), Article e3001081. https://doi.org/10.1371/journal.pbio.3001081

The apical complex is the instrument of invasion used by apicomplexan parasites, and the conoid is a conspicuous feature of this apparatus found throughout this phylum. The conoid, however, is believed to be heavily reduced or missing from Plasmodium... Read More about Molecular characterization of the conoid complex in Toxoplasma reveals its conservation in all apicomplexans, including Plasmodium species.

MRE11 Is Crucial for Malaria Parasite Transmission and Its Absence Affects Expression of Interconnected Networks of Key Genes Essential for Life (2020)
Journal Article
Guttery, D. S., Ramaprasad, A., Ferguson, D. J. P., Zeeshan, M., Pandey, R., Brady, D., Holder, A. A., Pain, A., & Tewari, R. (2020). MRE11 Is Crucial for Malaria Parasite Transmission and Its Absence Affects Expression of Interconnected Networks of Key Genes Essential for Life. Cells, 9(12), Article 2590. https://doi.org/10.3390/cells9122590

The meiotic recombination 11 protein (MRE11) plays a key role in DNA damage response and maintenance of genome stability. However, little is known about its function during development of the malaria parasite Plasmodium. Here, we present a functional... Read More about MRE11 Is Crucial for Malaria Parasite Transmission and Its Absence Affects Expression of Interconnected Networks of Key Genes Essential for Life.

A divergent cyclin/cyclin-dependent kinase complex controls the atypical replication of a malaria parasite during gametogony and transmission (2020)
Journal Article
Balestra, A. C., Zeeshan, M., Rea, E., Pasquarello, C., Brusini, L., Mourier, T., Subudhi, A. K., Klages, N., Arboit, P., Pandey, R., Brady, D., Vaughan, S., Holder, A. A., Pain, A., Ferguson, D. J., Hainard, A., Tewari, R., & Brochet, M. (2020). A divergent cyclin/cyclin-dependent kinase complex controls the atypical replication of a malaria parasite during gametogony and transmission. eLife, 9, Article e56474. https://doi.org/10.7554/eLife.56474

© 2020, Balestra et al. Cell cycle transitions are generally triggered by variation in the activity of cyclin-dependent kinases (CDKs) bound to cyclins. Malaria-causing parasites have a life cycle with unique cell-division cycles, and a repertoire of... Read More about A divergent cyclin/cyclin-dependent kinase complex controls the atypical replication of a malaria parasite during gametogony and transmission.

Plasmodium Condensin Core Subunits SMC2/SMC4 Mediate Atypical Mitosis and Are Essential for Parasite Proliferation and Transmission (2020)
Journal Article
Le Roch, K. G., Holder, A. A., Bottrill, A. R., Stanway, R. R., Wall, R. J., Tewari, R., Pandey, R., Abel, S., Boucher, M., Zeeshan, M., Wheatley, S., Batugedara, G., Hollin, T., Daniel, E., Rea, E., Freville, A., Lu, X. M., Gupta, D., & Brady, D. (2020). Plasmodium Condensin Core Subunits SMC2/SMC4 Mediate Atypical Mitosis and Are Essential for Parasite Proliferation and Transmission. Cell Reports, 30(6), 1883-1897.e6. https://doi.org/10.1016/j.celrep.2020.01.033

Condensin is a multi-subunit protein complex regulating chromosome condensation and segregation during cell division. In Plasmodium spp., the causative agent of malaria, cell division is atypical and the role of condensin is unclear. Here we examine... Read More about Plasmodium Condensin Core Subunits SMC2/SMC4 Mediate Atypical Mitosis and Are Essential for Parasite Proliferation and Transmission.

Plasmodium kinesin-8X associates with mitotic spindles and is essential for oocyst development during parasite proliferation and transmission (2019)
Journal Article
Zeeshan, M., Shilliday, F., Liu, T., Abel, S., Mourier, T., Ferguson, D. J. P., Rea, E., Stanway, R. R., Roques, M., Williams, D., Daniel, E., Brady, D., Roberts, A. J., Holder, A. A., Pain, A., Le, K. G., Le Roch, R. G., Moores, C. A., & Tewari, R. (2019). Plasmodium kinesin-8X associates with mitotic spindles and is essential for oocyst development during parasite proliferation and transmission. PLoS Pathogens, 15(10), Article e1008048. https://doi.org/10.1371/journal.ppat.1008048

Kinesin-8 proteins are microtubule motors that are often involved in regulation of mitotic spindle length and chromosome alignment. They move towards the plus ends of spindle microtubules and regulate the dynamics of these ends due, at least in some... Read More about Plasmodium kinesin-8X associates with mitotic spindles and is essential for oocyst development during parasite proliferation and transmission.