Outputs (16)

A Cdk1 phosphomimic mutant of MCAK impairs microtubule end recognition (2017)
Other
Belsham, H. R., & Friel, C. T. (2017). A Cdk1 phosphomimic mutant of MCAK impairs microtubule end recognition

The microtubule depolymerising kinesin-13, MCAK, is phosphorylated at residue T537 by Cdk1. This is the only known phosphorylation site within MCAK’s motor domain. To understand the impact of phosphorylation by Cdk1 microtubule depolymerisation activ... Read More about A Cdk1 phosphomimic mutant of MCAK impairs microtubule end recognition.

The family-specific α4-helix of the kinesin-13, MCAK, is critical to microtubule end recognition (2016)
Journal Article
Patel, J. T., Belsham, H. R., Rathbone, A. J., Wickstead, B., Gell, C., & Friel, C. T. (2016). The family-specific α4-helix of the kinesin-13, MCAK, is critical to microtubule end recognition. Open Biology, 6(10), Article 160223. https://doi.org/10.1098/rsob.160223

Kinesins that influence the dynamics of microtubule growth and shrinkage require the ability to distinguish between the microtubule end and the microtubule lattice. The microtubule depolymerizing kinesin MCAK has been shown to specifically recognize... Read More about The family-specific α4-helix of the kinesin-13, MCAK, is critical to microtubule end recognition.

Use of stopped-flow fluorescence and labeled nucleotides to analyze the ATP turnover cycle of kinesins (2014)
Journal Article
Patel, J. T., Belsham, H. R., Rathbone, A. J., & FRIEL, C. (2014). Use of stopped-flow fluorescence and labeled nucleotides to analyze the ATP turnover cycle of kinesins. Journal of Visualized Experiments, Article e52142. https://doi.org/10.3791/52142

Coupling of kinesin ATP turnover to translocation and microtubule regulation: One engine, many machines (2012)
Journal Article
Friel, C. T., & Howard, J. (2012). Coupling of kinesin ATP turnover to translocation and microtubule regulation: One engine, many machines. Journal of Muscle Research and Cell Motility, 33(6), 377-383. https://doi.org/10.1007/s10974-012-9289-6

The cycle of ATP turnover is integral to the action of motor proteins. Here we discuss how variation in this cycle leads to variation of function observed amongst members of the kinesin superfamily of microtubule associated motor proteins. Variation... Read More about Coupling of kinesin ATP turnover to translocation and microtubule regulation: One engine, many machines.

Mitotic centromere-associated kinesin (MCAK): a potential cancer drug target (2011)
Journal Article
Sanhaji, M., Friel, C. T., Wordeman, L., Louwen, F., & Yuan, J. (2011). Mitotic centromere-associated kinesin (MCAK): a potential cancer drug target. Oncotarget, 2(12), 935-947. https://doi.org/10.18632/oncotarget.416

The inability to faithfully segregate chromosomes in mitosis results in chromosome instability, a hallmark of solid tumors. Disruption of microtubule dynamics contributes highly to mitotic chromosome instability. The kinesin-13 family is critical in... Read More about Mitotic centromere-associated kinesin (MCAK): a potential cancer drug target.

The kinesin-13 MCAK has an unconventional ATPase cycle adapted for microtubule depolymerization (2011)
Journal Article
Friel, C. T., & Howard, J. (2011). The kinesin-13 MCAK has an unconventional ATPase cycle adapted for microtubule depolymerization. EMBO Journal, 30(19), 3928-3939. https://doi.org/10.1038/emboj.2011.290

Unlike other kinesins, members of the kinesin-13 subfamily do not move directionally along microtubules but, instead, depolymerize them. To understand how kinesins with structurally similar motor domains can have such dissimilar functions, we elucida... Read More about The kinesin-13 MCAK has an unconventional ATPase cycle adapted for microtubule depolymerization.