Outputs (11)

An optimised toolkit for high molecular weight DNA extraction and ultra-long-read nanopore sequencing using glass beads and hexamminecobalt(III) chloride (2025)
Journal Article
Cahyani, I., Tyson, J., Holmes, N., Quick, J., Moore, C., Loman, N., & Loose, M. (2025). An optimised toolkit for high molecular weight DNA extraction and ultra-long-read nanopore sequencing using glass beads and hexamminecobalt(III) chloride. Genome Research, 35(5), 1154-1166. https://doi.org/10.1101/gr.279943.124

Since the advent of long-read sequencing, achieving longer read lengths has been a key goal for many users. Ultra-long-read sets (N50 ≥ 100 kb) produced from Oxford Nanopore sequencers have improved genome assemblies in recent years. However, despite... Read More about An optimised toolkit for high molecular weight DNA extraction and ultra-long-read nanopore sequencing using glass beads and hexamminecobalt(III) chloride.

Impact of whole-genome duplications on structural variant evolution in Cochlearia (2024)
Journal Article
Hämälä, T., Moore, C., Cowan, L., Carlile, M., Gopaulchan, D., K. Brandrud, M. K., Birkeland, S., Loose, M., Kolář, F., Koch, M., & Yant, L. (2024). Impact of whole-genome duplications on structural variant evolution in Cochlearia. Nature Communications, 15, Article 5377. https://doi.org/10.1038/s41467-024-49679-y

Polyploidy, the result of whole-genome duplication (WGD), is a major driver of eukaryote evolution. Yet WGDs are hugely disruptive mutations, and we still lack a clear understanding of their fitness consequences. Here, we study whether WGDs result in... Read More about Impact of whole-genome duplications on structural variant evolution in Cochlearia.

A genomic toolkit for winged bean Psophocarpus tetragonolobus (2024)
Journal Article
Ho, W. K., Tanzi, A. S., Sang, F., Tsoutsoura, N., Shah, N., Moore, C., Bhosale, R., Wright, V., Massawe, F., & Mayes, S. (2024). A genomic toolkit for winged bean Psophocarpus tetragonolobus. Nature Communications, 15(1), Article 1901. https://doi.org/10.1038/s41467-024-45048-x

A sustainable supply of plant protein is critical for future generations and needs to be achieved while reducing green house gas emissions from agriculture and increasing agricultural resilience in the face of climate volatility. Agricultural diversi... Read More about A genomic toolkit for winged bean Psophocarpus tetragonolobus.

Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus (2023)
Journal Article
Edwards, A., Njaci, I., Sarkar, A., Jiang, Z., Kaithakottil, G. G., Moore, C., Cheema, J., Stevenson, C. E. M., Rejzek, M., Novák, P., Vigouroux, M., Vickers, M., Wouters, R. H. M., Paajanen, P., Steuernagel, B., Moore, J. D., Higgins, J., Swarbreck, D., Martens, S., Kim, C. Y., …Emmrich, P. M. F. (2023). Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus. Nature Communications, 14, Article 876. https://doi.org/10.1038/s41467-023-36503-2

Grass pea (Lathyrus sativus L.) is a rich source of protein cultivated as an insurance crop in Ethiopia, Eritrea, India, Bangladesh, and Nepal. Its resilience to both drought and flooding makes it a promising crop for ensuring food security in a chan... Read More about Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus.

The Impact of Real-Time Whole-Genome Sequencing in Controlling Healthcare-Associated SARS-CoV-2 Outbreaks (2021)
Journal Article
Francis, R. V., Billam, H., Clarke, M., Yates, C., Tsoleridis, T., Berry, L., Mahida, N., Irving, W. L., Moore, C., Holmes, N., Ball, J. K., Loose, M., & McClure, C. P. (2022). The Impact of Real-Time Whole-Genome Sequencing in Controlling Healthcare-Associated SARS-CoV-2 Outbreaks. Journal of Infectious Diseases, 225(1), 10-18. https://doi.org/10.1093/infdis/jiab483

Nosocomial severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections have severely affected bed capacity and patient flow. We utilized whole-genome sequencing (WGS) to identify outbreaks and focus infection control resources and interve... Read More about The Impact of Real-Time Whole-Genome Sequencing in Controlling Healthcare-Associated SARS-CoV-2 Outbreaks.

Study on Significance of Receptor Targeting in Killing of Intracellular Bacteria with Membrane-Impermeable Antibiotics (2021)
Journal Article
Catania, R., Mastrotto, F., Moore, C. J., Bosquillon, C., Falcone, F. H., Huett, A., Mantovani, G., & Stolnik, S. (2021). Study on Significance of Receptor Targeting in Killing of Intracellular Bacteria with Membrane-Impermeable Antibiotics. Advanced Therapeutics, 4(12), Article 2100168. https://doi.org/10.1002/adtp.202100168

Water-soluble antibiotics are largely excluded from therapy of intracellular infections, such as Shigella spp., Listeria spp., or Salmonella spp., due to their inability to permeate mammalian cell membrane. Here, the authors study if targeting offers... Read More about Study on Significance of Receptor Targeting in Killing of Intracellular Bacteria with Membrane-Impermeable Antibiotics.

Magnesium and calcium overaccumulate in the leaves of a schengen3 mutant of Brassica rapa (2021)
Journal Article
Alcock, T. D., Thomas, C. L., Ó Lochlainn, S., Pongrac, P., Wilson, M., Moore, C., Reyt, G., Vogel-Mikuš, K., Kelemen, M., Hayden, R., Wilson, L., Stephenson, P., Østergaard, L., Irwin, J. A., Hammond, J. P., King, G. J., Salt, D. E., Graham, N. S., White, P. J., & Broadley, M. R. (2021). Magnesium and calcium overaccumulate in the leaves of a schengen3 mutant of Brassica rapa. Plant Physiology, 186(3), 1616-1631. https://doi.org/10.1093/PLPHYS/KIAB150

Magnesium (Mg) and calcium (Ca) are essential mineral nutrients poorly supplied in many human food systems. In grazing livestock, Mg and Ca deficiencies are costly welfare issues. Here, we report a Brassica rapa loss-of-function schengen3 (sgn3) muta... Read More about Magnesium and calcium overaccumulate in the leaves of a schengen3 mutant of Brassica rapa.

Engulfment, persistance and fate of Bdellovibrio bacteriovorus predators inside human phagocytic cells informs their future therapeutic potential (2019)
Journal Article
Raghunathan, D., Radford, P. M., Gell, C., Negus, D., Moore, C., Till, R., Tighe, P. J., Wheatley, S. P., Martinez-Pomares, L., Elizabeth Sockett, R., & Tyson, J. (2019). Engulfment, persistance and fate of Bdellovibrio bacteriovorus predators inside human phagocytic cells informs their future therapeutic potential. Scientific Reports, 9, 1-16. https://doi.org/10.1038/s41598-019-40223-3

In assessing the potential of predatory bacteria, such as Bdellovibrio bacteriovorus, to become live therapeutic agents against bacterial infections, it is crucial to understand and quantify Bdellovibrio host cell interactions at a molecular level. H... Read More about Engulfment, persistance and fate of Bdellovibrio bacteriovorus predators inside human phagocytic cells informs their future therapeutic potential.

Measuring and modelling the response of Klebsiella pneumoniae KPC prey to Bdellovibrio bacteriovorus predation, in human serum and defined buffer (2017)
Journal Article
Baker, M., Negus, D., Raghunathan, D., Radford, P., Moore, C., Clark, G., Diggle, M., Tyson, J., Twycross, J., & Sockett, R. E. (in press). Measuring and modelling the response of Klebsiella pneumoniae KPC prey to Bdellovibrio bacteriovorus predation, in human serum and defined buffer. Scientific Reports, 7(8329), https://doi.org/10.1038/s41598-017-08060-4

In worldwide conditions of increasingly antibiotic-resistant hospital infections, it is important to research alternative therapies. Bdellovibrio bacteriovorus bacteria naturally prey on Gram-negative pathogens, including antibiotic-resistant strains... Read More about Measuring and modelling the response of Klebsiella pneumoniae KPC prey to Bdellovibrio bacteriovorus predation, in human serum and defined buffer.

Tropomyosin 1: multiple roles in the developing heart and in the formation of congenital heart defects (2017)
Journal Article
England, J., Granados-Riveron, J. T., Polo-Parada, L., Kuriakose, D., Moore, C., Brook, J. D., Rutland, C. S., Setchfield, K., Gell, C., Ghosh, T. K., Bu'Lock, F. A., Thornborough, C., Ehler, E., & Loughna, S. (2017). Tropomyosin 1: multiple roles in the developing heart and in the formation of congenital heart defects. Journal of Molecular and Cellular Cardiology, 106, https://doi.org/10.1016/j.yjmcc.2017.03.006

Tropomyosin 1 (TPM1) is an essential sarcomeric component, stabilising the thin filament and facilitating actin's interaction with myosin. A number of sarcomeric proteins, such as alpha myosin heavy chain, play crucial roles in cardiac development. M... Read More about Tropomyosin 1: multiple roles in the developing heart and in the formation of congenital heart defects.