Dr YING ZHANG's Outputs (17)

Profiling of RNA N6-Methyladenosine methylation reveals the critical role of m6A in betaine alleviating hepatic steatosis (2025)
Journal Article
Wu, Y., Yu, J., Song, H., Lu, D., Li, Z., Li, X., Ding, Z., Zhou, L., Ma, J., Zhang, Y., & Li, Y. (2025). Profiling of RNA N6-Methyladenosine methylation reveals the critical role of m6A in betaine alleviating hepatic steatosis. Scientific Reports, 15, Article 7298. https://doi.org/10.1038/s41598-025-91573-0

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a major predisposing factor of metabolic dysfunction-associated steatohepatitis, hepatic fibrosis and hepatocellular carcinoma. Abnormal RNA m6A modification leads to the disturbance... Read More about Profiling of RNA N6-Methyladenosine methylation reveals the critical role of m6A in betaine alleviating hepatic steatosis.

Development of a Suite of Tools for Genome Editing in Parageobacillus thermoglucosidasius and Their Use to Identify the Potential of a Native Plasmid in the Generation of Stable Engineered Strains (2021)
Journal Article
Lau, M. S. H., Sheng, L., Zhang, Y., & Minton, N. P. (2021). Development of a Suite of Tools for Genome Editing in Parageobacillus thermoglucosidasius and Their Use to Identify the Potential of a Native Plasmid in the Generation of Stable Engineered Strains. ACS Synthetic Biology, 10(7), 1739-1749. https://doi.org/10.1021/acssynbio.1c00138

The relentless rise in the levels of atmospheric greenhouse gases caused by the exploitation of fossil fuel necessitates the development of more environmentally friendly routes to the manufacture of chemicals and fuels. The exploitation of a fermenta... Read More about Development of a Suite of Tools for Genome Editing in Parageobacillus thermoglucosidasius and Their Use to Identify the Potential of a Native Plasmid in the Generation of Stable Engineered Strains.

Development of Clostridium saccharoperbutylacetonicum as a Whole Cell Biocatalyst for Production of Chirally Pure (R)-1,3-Butanediol (2021)
Journal Article
Grosse-Honebrink, A., Little, G. T., Bean, Z., Heldt, D., Cornock, R. H. M., Winzer, K., Minton, N. P., Green, E., & Zhang, Y. (2021). Development of Clostridium saccharoperbutylacetonicum as a Whole Cell Biocatalyst for Production of Chirally Pure (R)-1,3-Butanediol. Frontiers in Bioengineering and Biotechnology, 9, Article 659895. https://doi.org/10.3389/fbioe.2021.659895

Chirally pure (R)-1,3-butanediol ((R)-1,3-BDO) is a valuable intermediate for the production of fragrances, pheromones, insecticides and antibiotics. Biotechnological production results in superior enantiomeric excess over chemical production and is... Read More about Development of Clostridium saccharoperbutylacetonicum as a Whole Cell Biocatalyst for Production of Chirally Pure (R)-1,3-Butanediol.

Engineering Geobacillus thermoglucosidasius for direct utilisation of holocellulose from wheat straw (2019)
Journal Article
Bashir, Z., Sheng, L., Anil, A., Lali, A., Minton, N. P., & Zhang, Y. (2019). Engineering Geobacillus thermoglucosidasius for direct utilisation of holocellulose from wheat straw. Biotechnology for Biofuels, 12(1), Article 199. https://doi.org/10.1186/s13068-019-1540-6

Background: A consolidated bioprocessing (CBP), where lignocellulose is converted into the desired product(s) in a single fermentative step without the addition of expensive degradative enzymes, represents the ideal solution of renewable routes to ch... Read More about Engineering Geobacillus thermoglucosidasius for direct utilisation of holocellulose from wheat straw.

Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants (2017)
Journal Article
Grosse-Honebrink, A., Schwarz, K., Wang, H., Minton, N. P., & Zhang, Y. (2017). Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants. Anaerobe, 48, https://doi.org/10.1016/j.anaerobe.2017.09.001

Effective microbial metabolic engineering is reliant on efficient gene transfer. Here we present a simple screening strategy that may be deployed to isolate rare, hypertransformable variants. The procedure was used to increase the frequency of transf... Read More about Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants.

Breeding histories and selection criteria for oilseed rape in Europe and China identified by genome wide pedigree dissection (2017)
Journal Article
Wang, X., Long, Y., Wang, N., Zou, J., Ding, G., Broadley, M. R., White, P. J., Yuan, P., Zhang, Q., Luo, Z., Liu, P., Zhao, H., Zhang, Y., Cai, H., King, G. J., Xu, F., Meng, J., & Shi, L. (2017). Breeding histories and selection criteria for oilseed rape in Europe and China identified by genome wide pedigree dissection. Scientific Reports, 7(1), Article 1916. https://doi.org/10.1038/s41598-017-02188-z

Selection breeding has played a key role in the improvement of seed yield and quality in oilseed rape (Brassica napus L.). We genotyped Tapidor (European), Ningyou7 (Chinese) and their progenitors with the Brassica 60 K Illumina Infinium SNP array an... Read More about Breeding histories and selection criteria for oilseed rape in Europe and China identified by genome wide pedigree dissection.

Towards improved butanol production through targeted genetic modification of Clostridium pasteurianum (2017)
Journal Article
Schwarz, K., Grosse-Honebrink, A., Derecka, K., Rotta, C., Zhang, Y., & Minton, N. P. (2017). Towards improved butanol production through targeted genetic modification of Clostridium pasteurianum. Metabolic Engineering, 40, 124-137. https://doi.org/10.1016/j.ymben.2017.01.009

Declining fossil fuel reserves, coupled with environmental concerns over their continued extraction and exploitation have led to strenuous efforts to identify renewable routes to energy and fuels. One attractive option is to convert glycerol, a by-pr... Read More about Towards improved butanol production through targeted genetic modification of Clostridium pasteurianum.

Development and implementation of rapid metabolic engineering tools for chemical and fuel production in Geobacillus thermoglucosidasius NCIMB 11955 (2017)
Journal Article
Sheng, L., Kovács, K., Winzer, K., Zhang, Y., & Minton, N. P. (2017). Development and implementation of rapid metabolic engineering tools for chemical and fuel production in Geobacillus thermoglucosidasius NCIMB 11955. Biotechnology for Biofuels, 10(1), Article 5. https://doi.org/10.1186/s13068-016-0692-x

Background

The thermophile Geobacillus thermoglucosidasius has considerable attraction as a chassis for the production of chemicals and fuels. It utilises a wide range of sugars and oligosaccharides typical of those derived from lignocellulose and... Read More about Development and implementation of rapid metabolic engineering tools for chemical and fuel production in Geobacillus thermoglucosidasius NCIMB 11955.

Enhanced solvent production by metabolic engineering of a twin-clostridial consortium (2016)
Journal Article
Wen, Z., Minton, N. P., Zhang, Y., Li, Q., Liu, J., Jiang, Y., & Yang, S. (2017). Enhanced solvent production by metabolic engineering of a twin-clostridial consortium. Metabolic Engineering, 39, 38-48. https://doi.org/10.1016/j.ymben.2016.10.013

QTL meta-analysis of root traits in Brassica napus under contrasting phosphorus supply in two growth systems (2016)
Journal Article
Zhang, Y., Thomas, C. L., Xiang, J., Long, Y., Wang, X., Zou, J., Luo, Z., Ding, G., Cai, H., Graham, N. S., Hammond, J. P., King, G. J., White, P. J., Xu, F., Broadley, M. R., Shi, L., & Meng, J. (2016). QTL meta-analysis of root traits in Brassica napus under contrasting phosphorus supply in two growth systems. Scientific Reports, 6(1), Article 33113. https://doi.org/10.1038/srep33113

© The Author(s) 2016. A high-density SNP-based genetic linkage map was constructed and integrated with a previous map in the Tapidor x Ningyou7 (TNDH) Brassica napus population, giving a new map with a total of 2041 molecular markers and an average m... Read More about QTL meta-analysis of root traits in Brassica napus under contrasting phosphorus supply in two growth systems.

A roadmap for gene system development in Clostridium (2016)
Journal Article
Minton, N. P., Ehsaan, M., Humphreys, C. M., Little, G. T., Baker, J., Henstra, A. M., Liew, F., Kelly, M., Sheng, L., Schwarz, K., & Zhang, Y. (2016). A roadmap for gene system development in Clostridium. Anaerobe, 41, 104-112. https://doi.org/10.1016/j.anaerobe.2016.05.011

Clostridium species are both heroes and villains. Some cause serious human and animal diseases, those present in the microbiota contribute to health and wellbeing, while others represent useful industrial chassis for the production of chemicals and f... Read More about A roadmap for gene system development in Clostridium.

CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii (2016)
Journal Article
Li, Q., Cheng, J., Minton, N. P., Zhang, Y., Wen, Z., Liu, J., Yang, H., Zeng, Z., Ren, X., Yang, J., Gu, Y., Jiang, W., Jiang, Y., & Yang, S. (2016). CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii. Biotechnology Journal, 11(7), 961-972. https://doi.org/10.1002/biot.201600053

Solventogenic clostridia are important industrial microorganisms that produce various chemicals and fuels. Effective genetic tools would facilitate physiological studies aimed both at improving our understanding of metabolism and optimizing solvent p... Read More about CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii.

Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum (2016)
Journal Article
Zhang, Y., Xu, S., Chai, C., Yang, S., Jiang, W., Minton, N. P., & Gu, Y. (2016). Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum. FEMS Microbiology Letters, 363(8), Article fnw065. https://doi.org/10.1093/femsle/fnw065

Clostridium acetobutylicum is an industrially important Gram-positive organism which is capable of producing economically important chemicals in the ABE (Acetone, Butanol and Ethanol) fermentation process. Renewed interests in the ABE process necessi... Read More about Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum.

Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824 (2016)
Journal Article
Ehsaan, M., Kuit, W., Zhang, Y., Cartman, S. T., Heap, J. T., Winzer, K., & Minton, N. P. (2016). Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824. Biotechnology for Biofuels, 9(1), Article 4. https://doi.org/10.1186/s13068-015-0410-0

Background

Clostridium acetobutylicum represents a paradigm chassis for the industrial production of the biofuel biobutanol and a focus for metabolic engineering. We have previously developed procedures for the creation of in-frame, marker-less de... Read More about Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824.

A universal mariner transposon system for forward genetic studies in the genus Clostridium (2015)
Journal Article
Zhang, Y., Grosse-Honebrink, A., & Minton, N. P. (2015). A universal mariner transposon system for forward genetic studies in the genus Clostridium. PLoS ONE, 10(4), 1-21. https://doi.org/10.1371/journal.pone.0122411

DNA transposons represent an essential tool in the armoury of the molecular microbiologist. We previously developed a catP-based mini transposon system for Clostridium difficile in which the expression of the transposase gene was dependent on a sigma... Read More about A universal mariner transposon system for forward genetic studies in the genus Clostridium.

Structural evidence that colicin a protein binds to a novel binding site of TolA protein in Escherichia coli periplasm (2012)
Journal Article
Li, C., Zhang, Y., Vankemmelbeke, M., Hecht, O., Aleanizy, F. S., Macdonald, C., Moore, G. R., James, R., & Penfold, C. N. (2012). Structural evidence that colicin a protein binds to a novel binding site of TolA protein in Escherichia coli periplasm. Journal of Biological Chemistry, 287(23), https://doi.org/10.1074/jbc.M112.342246

The Tol assembly of proteins is an interacting network of proteins located in the Escherichia coli cell envelope that transduces energy and contributes to cell integrity. TolA is central to this network linking the inner and outer membranes by intera... Read More about Structural evidence that colicin a protein binds to a novel binding site of TolA protein in Escherichia coli periplasm.

Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors (2004)
Journal Article
Ahmed, B., Chakravarthy, S., Eggers, R., Hermens, W., Zhang, J., Niclou, S., Levelt, C., Sablitzky, F., Anderson, P., Lieberman, A., & Verhaagen, J. (2004). Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors. BMC Neuroscience, 5(1), Article 4. https://doi.org/10.1186/1471-2202-5-4

BACKGROUND: Inactivating genes in vivo is an important technique for establishing their function in the adult nervous system. Unfortunately, conventional knockout mice may suffer from several limitations including embryonic or perinatal lethality and... Read More about Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors.