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Dr LISA WHITE's Outputs (39)

Translational considerations in injectable cell-based therapeutics for neurological applications: concepts, progress and challenges (2017)
Journal Article
Amer, M. H., Rose, F. R., Shakesheff, K. M., Modo, M., & White, L. J. (in press). Translational considerations in injectable cell-based therapeutics for neurological applications: concepts, progress and challenges. npj Regenerative Medicine, 2, Article 23. https://doi.org/10.1038/s41536-017-0028-x

Significant progress has been made during the past decade towards the clinical adoption of cell- based therapeutics. However, existing cell delivery approaches have shown limited success, with numerous studies showing fewer than 5% of injected cells... Read More about Translational considerations in injectable cell-based therapeutics for neurological applications: concepts, progress and challenges.

Decellularized bone extracellular matrix and human dental pulp stem cells as a construct for bone regeneration (2017)
Journal Article
Paduano, F., Marrelli, M., Alom, N., Amer, M. H., White, L. J., Shakesheff, K. M., & Tatullo, M. (in press). Decellularized bone extracellular matrix and human dental pulp stem cells as a construct for bone regeneration. Journal of Biomaterials Science, Polymer Edition, 28(8), https://doi.org/10.1080/09205063.2017.1301770

Dental pulp tissue represents a source of mesenchymal stem cells (MSCs) that have a strong differentiation potential towards the osteogenic lineage. The objective of the current study was to examine in vitro osteogenic induction of dental pulp stem c... Read More about Decellularized bone extracellular matrix and human dental pulp stem cells as a construct for bone regeneration.

Macrophage phenotype in response to ECM bioscaffolds (2017)
Journal Article
Huleihel, L., Dziki, J. L., Bartolacci, J. G., Rausch, T., Scarritt, M. E., Cramer, M. C., Vorobyov, T., LoPresti, S. T., Swinehart, I. T., White, L. J., Brown, B. N., & Badylak, S. F. (2017). Macrophage phenotype in response to ECM bioscaffolds. Seminars in Immunology, 29, https://doi.org/10.1016/j.smim.2017.04.004

Macrophage presence and phenotype are critical determinants of the healing response following injury. Downregulation of the pro-inflammatory macrophage phenotype has been associated with the therapeutic use of bioscaffolds composed of extracellular... Read More about Macrophage phenotype in response to ECM bioscaffolds.

Surface modification of PdlLGA microspheres with gelatine methacrylate: evaluation of adsorption, entrapment, and oxygen plasma treatment approaches (2017)
Journal Article
Baki, A., Rahman, C. V., White, L. J., Scurr, D. J., Qutachi, O., & Shakesheff, K. M. (2017). Surface modification of PdlLGA microspheres with gelatine methacrylate: evaluation of adsorption, entrapment, and oxygen plasma treatment approaches. Acta Biomaterialia, 53, 450-459. https://doi.org/10.1016/j.actbio.2017.01.042

Injectable poly (dl-lactic-co-glycolic acid) (PdlLGA) microspheres are promising candidates as biodegradable controlled release carriers for drug and cell delivery applications; however, they have limited functional groups on the surface to enable de... Read More about Surface modification of PdlLGA microspheres with gelatine methacrylate: evaluation of adsorption, entrapment, and oxygen plasma treatment approaches.

The impact of detergents on the tissue decellularization process: a ToF-SIMS study (2016)
Journal Article
White, L. J., Taylor, A. J., Faulk, D. M., Keane, T. J., Saldin, L. T., Reing, J. E., Swinehart, I. T., Turner, N. J., Ratner, B. D., & Badylak, S. F. (2017). The impact of detergents on the tissue decellularization process: a ToF-SIMS study. Acta Biomaterialia, 50, https://doi.org/10.1016/j.actbio.2016.12.033

Biologic scaffolds are derived from mammalian tissues, which must be decellularized to remove cellular antigens that would otherwise incite an adverse immune response. Although widely used clinically, the optimum balance between cell removal and the... Read More about The impact of detergents on the tissue decellularization process: a ToF-SIMS study.

Extracellular matrix hydrogels from decellularized tissues: structure and function (2016)
Journal Article
Saldin, L. T., Cramer, M. C., Velankar, S. S., White, L. J., & Badylak, S. F. (2017). Extracellular matrix hydrogels from decellularized tissues: structure and function. Acta Biomaterialia, 49, https://doi.org/10.1016/j.actbio.2016.11.068

Extracellular matrix (ECM) bioscaffolds prepared from decellularized tissues have been used to facilitate constructive and functional tissue remodeling in a variety of clinical applications. The discovery that these ECM materials could be solubilized... Read More about Extracellular matrix hydrogels from decellularized tissues: structure and function.

Extracellular matrix-derived hydrogels for dental stem cell delivery (2016)
Journal Article
Viswanath, A., Vanacker, J., Germain, L., Leprince, J. G., Diogenes, A., Shakesheff, K. M., White, L. J., & des Rieux, A. (2016). Extracellular matrix-derived hydrogels for dental stem cell delivery. Journal of Biomedical Materials Research Part A, 105(1), 319-328. https://doi.org/10.1002/jbm.a.35901

Decellularised mammalian extracellular matrices (ECM) have been widely accepted as an ideal substrate for repair and remodelling of numerous tissues in clinical and pre-clinical studies. Recent studies have demonstrated the ability of ECM scaffolds d... Read More about Extracellular matrix-derived hydrogels for dental stem cell delivery.

Restoring Mucosal Barrier Function and Modifying Macrophage Phenotype with an Extracellular Matrix Hydrogel: Potential Therapy for Ulcerative Colitis (2016)
Journal Article
Keane, T. J., Dziki, J., Sobieski, E., Smoulder, A., Castleton, A. A., Turner, N. J., White, L. J., & Badylak, S. F. (2017). Restoring Mucosal Barrier Function and Modifying Macrophage Phenotype with an Extracellular Matrix Hydrogel: Potential Therapy for Ulcerative Colitis. Journal of Crohn's and Colitis, 11(3), 360-368. https://doi.org/10.1093/ecco-jcc/jjw149

Background and Aims: Despite advances in therapeutic options, more than half of all patients with ulcerative colitis [UC] do not achieve long-term remission, many require colectomy, and the disease still has a marked negative impact on quality of lif... Read More about Restoring Mucosal Barrier Function and Modifying Macrophage Phenotype with an Extracellular Matrix Hydrogel: Potential Therapy for Ulcerative Colitis.

A detailed assessment of varying ejection rate on delivery efficiency of mesenchymal stem cells using narrow-bore needles (2016)
Journal Article
Rose, F., Shakesheff, K., White, L., & Amer, M. (2016). A detailed assessment of varying ejection rate on delivery efficiency of mesenchymal stem cells using narrow-bore needles. Stem Cells Translational Medicine, 5(3), 366-378. https://doi.org/10.5966/sctm.2015-0208

As the number of clinical trials exploring cell therapy rises, a thorough understanding of the limits of cell delivery is essential. We used an extensive toolset comprising various standard and multiplex assays for the assessment of cell delivery pos... Read More about A detailed assessment of varying ejection rate on delivery efficiency of mesenchymal stem cells using narrow-bore needles.

Odontogenic differentiation of human dental pulp stem cells on hydrogel scaffolds derived from decellularized bone extracellular matrix and collagen type I (2016)
Journal Article
Paduano, F., Marrelli, M., White, L. J., Shakesheff, K., & Tatullo, M. (2016). Odontogenic differentiation of human dental pulp stem cells on hydrogel scaffolds derived from decellularized bone extracellular matrix and collagen type I. PLoS ONE, 11(2), 1-18. https://doi.org/10.1371/journal.pone.0148225

Objectives
The aim of this study was to evaluate the level of odontogenic
differentiation of dental pulp stem cells (DPSCs) on hydrogel scaffolds derived from bone extracellular matrix (bECM) in comparison to those seeded on collagen I (Col-I), on... Read More about Odontogenic differentiation of human dental pulp stem cells on hydrogel scaffolds derived from decellularized bone extracellular matrix and collagen type I.

In vivo assessment of bone regeneration in alginate/bone ECM hydrogels with incorporated skeletal stem cells and single growth factors (2015)
Journal Article
Gothard, D., Smith, E. L., Kanczler, J. M., Black, C. R., Wells, J. A., Roberts, C. A., White, L., Qutachi, O., Peto, H., Rashidi, H., Rojo, L., Stevens, M. M., El Haj, A. J., Rose, F., Shakesheff, K., & Oreffo, R. O. (2015). In vivo assessment of bone regeneration in alginate/bone ECM hydrogels with incorporated skeletal stem cells and single growth factors. PLoS ONE, 10(12), Article e0145080. https://doi.org/10.1371/journal.pone.0145080

A Thermoresponsive and Magnetic Colloid for 3D Cell Expansion and Reconfiguration (2014)
Journal Article
Saunders, B. R., Gould, T., Saeed, A. O., Francini, N., White, L., Dixon, J. E., Gould, T. W., Rashidi, H., Al Ghanami, R. C., Hruschka, V., Redl, H., Saunders, B. R., Alexander, C., & Shakesheff, K. M. (2015). A Thermoresponsive and Magnetic Colloid for 3D Cell Expansion and Reconfiguration. Advanced Materials, 27(4), 662-668. https://doi.org/10.1002/adma.201403626

A dual thermoresponsive and magnetic colloidal gel matrix is described for enhanced stem-cell culture. The combined properties of the material allow enzyme-free passaging and expansion of mesenchymal stem cells, as well as isolation of cells postcult... Read More about A Thermoresponsive and Magnetic Colloid for 3D Cell Expansion and Reconfiguration.

Formulations for modulation of protein release from large-size PLGA microparticles for tissue engineering (2014)
Journal Article
Qodratnama, R., Pio Serino, L., Cox, H., Qutachi, O., & White, L. J. (2015). Formulations for modulation of protein release from large-size PLGA microparticles for tissue engineering. Materials Science and Engineering: C, 47, 230-236. https://doi.org/10.1016/j.msec.2014.11.003

In this study we present an approach to pre-program lysozyme release from large size (100–300 μm) poly(dl-lactic acid-co-glycolic acid) (PLGA) microparticles. This approach involved blending in-house synthesized triblock copolymers with a PLGA 85:15.... Read More about Formulations for modulation of protein release from large-size PLGA microparticles for tissue engineering.

Evaluation of skeletal tissue repair, Part 1: Assessment of novel growth-factor-releasing hydrogels in an ex vivo chick femur defect model (2014)
Journal Article
Smith, E., Rose, F., Kanczler, J., Shakesheff, K., Gothard, D., White, L., Roberts, C., Wells, J., Qutachi, O., Sawkins, M., Peto, H., Rashidi, H., Rojocd, L., Stevens, M., El Haj, A., & Oreffoa, R. (2014). Evaluation of skeletal tissue repair, Part 1: Assessment of novel growth-factor-releasing hydrogels in an ex vivo chick femur defect model. Acta Biomaterialia, 10(10), 4186-4196. https://doi.org/10.1016/j.actbio.2014.06.011

Current clinical treatments for skeletal conditions resulting in large-scale bone loss include autograft or allograft, both of which have limited effectiveness. In seeking to address bone regeneration, several tissue engineering strategies have come... Read More about Evaluation of skeletal tissue repair, Part 1: Assessment of novel growth-factor-releasing hydrogels in an ex vivo chick femur defect model.

A biodegradable antibiotic-impregnated scaffold to prevent osteomyelitis in a contaminated in vivo bone defect model (2014)
Journal Article
McLaren, J. S., White, L., Cox, H., Ashraf, W., Rahman, C., Blunn, G., Goodship, A., Quirk, R., Shakesheff, K. M., Bayston, R., & Scammell, B. E. (2014). A biodegradable antibiotic-impregnated scaffold to prevent osteomyelitis in a contaminated in vivo bone defect model. eCells and Materials Journal, 27, 332-349. https://doi.org/10.22203/eCM.v027a24

Open fractures are at risk of serious infection and, if infected, require several surgical interventions and courses of systemic antibiotics. We investigated a new injectable formulation that simultaneously hardens in vivo to form a porous scaffold f... Read More about A biodegradable antibiotic-impregnated scaffold to prevent osteomyelitis in a contaminated in vivo bone defect model.

A biodegradable antibiotic-impregnated scaffold to prevent osteomyelitis and encourage new bone growth (2014)
Journal Article
McLaren, J. S., Bayston, R., White, L. J., Cox, H. C., Ashraf, W., Rahman, C. V., Blunn, G., Goodship, A. E., Quirk, R. A., Shakesheff, K. M., & Scammell, B. E. (2014). A biodegradable antibiotic-impregnated scaffold to prevent osteomyelitis and encourage new bone growth. eCells and Materials Journal, 27, 332-349. https://doi.org/10.22203/eCM.v027a24

Open fractures are at risk of serious infection and, if infected, require several surgical interventions and courses of systemic antibiotics. We investigated a new injectable formulation that simultaneously hardens in vivo to form a porous scaffold f... Read More about A biodegradable antibiotic-impregnated scaffold to prevent osteomyelitis and encourage new bone growth.

Evaluation of skeletal tissue repair, Part 2: Enhancement of skeletal tissue repair through dual-growth-factor-releasing hydrogels within an ex vivo chick femur defect model (2014)
Journal Article
Smith, E., Felicity, R., Kanczler, J., Shakesheff, K., Gothard, D., White, L., Roberts, C., Wells, J., Qutachi, O., Sawkins, M., Peto, H., Rashidi, H., Rojocd, L., Stevens, M., El Haj, A., & Oreffoa, R. (2014). Evaluation of skeletal tissue repair, Part 2: Enhancement of skeletal tissue repair through dual-growth-factor-releasing hydrogels within an ex vivo chick femur defect model. Acta Biomaterialia, 10(10), 4197-4205. https://doi.org/10.1016/j.actbio.2014.05.025

There is an unmet need for improved, effective tissue engineering strategies to replace or repair bone damaged through disease or injury. Recent research has focused on developing biomaterial scaffolds capable of spatially and temporally releasing co... Read More about Evaluation of skeletal tissue repair, Part 2: Enhancement of skeletal tissue repair through dual-growth-factor-releasing hydrogels within an ex vivo chick femur defect model.

Uniform cell colonization of porous 3-D scaffolds achieved using radial control of surface chemistry (2011)
Journal Article
Intranuovo, F., Howard, D., White, L. J., Johal, R. K., Ghaemmaghami, A. M., Favia, P., Howdle, S. M., Shakesheff, K. M., & Alexander, M. R. (2011). Uniform cell colonization of porous 3-D scaffolds achieved using radial control of surface chemistry. Acta Biomaterialia, 7(9), 3336-3344. https://doi.org/10.1016/j.actbio.2011.05.020

Uniform cellular distribution is a prerequisite to forming tissue within porous scaffolds, but the seeding process often results in preferential adhesion of cells at the periphery. We develop a vapour phase coating strategy which is readily applicabl... Read More about Uniform cell colonization of porous 3-D scaffolds achieved using radial control of surface chemistry.