Odontogenic differentiation of human dental pulp stem cells on hydrogel scaffolds derived from decellularized bone extracellular matrix and collagen type I

Paduano, Francesco; Marrelli, Massimo; White, Lisa J.; Shakesheff, Kevin; Tatullo, Marco

Abstract

Objectives
The aim of this study was to evaluate the level of odontogenic
differentiation of dental pulp stem cells (DPSCs) on hydrogel scaffolds derived from bone extracellular matrix (bECM) in comparison to those seeded on collagen I (Col-I), one of the main components of dental pulp ECM.

Methods
DPSCs isolated from human third molars were characterized for surface marker expression and odontogenic potential prior to seeding into bECM or Col-I hydrogel scaffolds. The cells were then seeded onto bECM and Col-I hydrogel scaffolds and cultured under basal conditions or with odontogenic and growth factor (GF) supplements. DPSCs cultivated on tissue culture polystyrene (TCPS) with and without supplements were used as controls. Gene expression of dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP-1) and matrix extracellular phosphoglycoprotein (MEPE) was evaluated by quantitative reverse transcription-polymerase chain reaction (qRT PCR) and mineral deposition was observed by Von Kossa staining.

Results
When DPSCs were cultured on bECM hydrogels, the mRNA expression levels of DSPP, DMP-1 and MEPE genes were significantly upregulated with respect to those cultured on Col-I scaffolds or TCPS in the absence of extra odontogenic inducers. In addition, more mineral deposition was observed on bECM hydrogel scaffolds as demonstrated by Von Kossa staining. Moreover, DSPP, DMP-1 and MEPE mRNA expressions of DPSCs cultured on bECM hydrogels were further upregulated by the addition of GFs or osteo/odontogenic
medium compared to Col-I treated cells in the same culture conditions.

Significance
These results demonstrate the potential of the bECM hydrogel scaffolds to stimulate odontogenic differentiation of DPSCs.

Citation

Paduano, F., Marrelli, M., White, L. J., Shakesheff, K., & Tatullo, M. (2016). Odontogenic differentiation of human dental pulp stem cells on hydrogel scaffolds derived from decellularized bone extracellular matrix and collagen type I. PLoS ONE, 11(2), 1-18. https://doi.org/10.1371/journal.pone.0148225

• Journal Article Type: Article
• Acceptance Date: Jan 14, 2016
• Online Publication Date: Feb 16, 2016
• Publication Date: Feb 16, 2016
• Deposit Date: Sep 14, 2018
• Publicly Available Date: Oct 31, 2018
• Journal: PLoS ONE
• Electronic ISSN: 1932-6203
• Publisher: Public Library of Science
• Peer Reviewed: Peer Reviewed
• Volume: 11
• Issue: 2
• Article Number: e0148225
• Pages: 1-18
• DOI: https://doi.org/10.1371/journal.pone.0148225
• Public URL: https://nottingham-repository.worktribe.com/output/1109277
• Publisher URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0148225
• PMID: 00037121