Skip to main content

Research Repository

Advanced Search

Dr Alexander Tarr's Outputs (74)

Determination of the human antibody response to the epitope defined by the hepatitis C virus-neutralizing monoclonal antibody AP33 (2007)
Journal Article
Tarr, A. W., Owsianka, A. M., Jayaraj, D., Brown, R. J., Hickling, T. P., Irving, W. L., Patel, A. H., & Ball, J. K. (2007). Determination of the human antibody response to the epitope defined by the hepatitis C virus-neutralizing monoclonal antibody AP33. Journal of General Virology, 88(11), 2991-3001. https://doi.org/10.1099/vir.0.83065-0

Hepatitis C virus (HCV) is a major cause of liver disease worldwide and there is a pressing need for the development of a preventative vaccine as well as new treatments. It was recently demonstrated that the mouse monoclonal antibody (mAb) AP33 poten... Read More about Determination of the human antibody response to the epitope defined by the hepatitis C virus-neutralizing monoclonal antibody AP33.

Human combinatorial libraries yield rare antibodies that broadly neutralize hepatitis C virus (2007)
Journal Article
Johansson, D. X., Voisset, C., Tarr, A. W., Aung, M., Ball, J. K., Dubuisson, J., & Persson, M. A. (2007). Human combinatorial libraries yield rare antibodies that broadly neutralize hepatitis C virus. Proceedings of the National Academy of Sciences, 104(41), 16269-16274. https://doi.org/10.1073/pnas.0705522104

One way to dissect the antibody response to an invading microorganism is to clone the antibody repertoire from immune donors and subsequently characterize the specific antibodies. Recently, methodological advances have allowed investigations of neutr... Read More about Human combinatorial libraries yield rare antibodies that broadly neutralize hepatitis C virus.

Cloning, expression, and functional analysis of patient-derived hepatitis C virus glycoproteins (2007)
Book Chapter
Tarr, A. W., Owsianka, A. M., Szwejk, A., Ball, J. K., & Patel, A. H. (2007). Cloning, expression, and functional analysis of patient-derived hepatitis C virus glycoproteins. In R. J. Sugrue (Ed.), Glycovirology Protocols (177-197). Humana Press. https://doi.org/10.1007/978-1-59745-393-6_13

Hepatitis C virus (HCV) infection is a major cause of severe chronic liver disease including cirrhosis and hepatocellular carcinoma. HCV has been classified into six major genotypes that exhibit extensive genetic variability, particularly in the enve... Read More about Cloning, expression, and functional analysis of patient-derived hepatitis C virus glycoproteins.

Cloning, Expression, and Functional Analysis of Patient-Derived Hepatitis C Virus Glycoproteins (2007)
Book Chapter
Tarr, A. W., Owsianka, A. M., Szwejk, A., Ball, J. K., & Patel, A. H. (2007). Cloning, Expression, and Functional Analysis of Patient-Derived Hepatitis C Virus Glycoproteins. In Glycovirology Protocols (177-197). Humana Press. https://doi.org/10.1385/1-59745-393-5%3A177

Hepatitis C virus (HCV) infection is a major cause of severe chronic liver disease including cirrhosis and hepatocellular carcinoma. HCV has been classified into six major genotypes that exhibit extensive genetic variability, particularly in the enve... Read More about Cloning, Expression, and Functional Analysis of Patient-Derived Hepatitis C Virus Glycoproteins.

Cloning, expression, and functional analysis of patient-derived hepatitis C virus glycoproteins (2007)
Book Chapter
Tarr, A. W., Owsianka, A. M., Szwejk, A., Ball, J. K., & Patel, A. H. (2007). Cloning, expression, and functional analysis of patient-derived hepatitis C virus glycoproteins. In R. J. Sugrue (Ed.), Glycovirology Protocols (177-197). Humana Press. https://doi.org/10.1007/978-1-59745-393-6_13

Hepatitis C virus (HCV) infection is a major cause of severe chronic liver disease including cirrhosis and hepatocellular carcinoma. HCV has been classified into six major genotypes that exhibit extensive genetic variability, particularly in the enve... Read More about Cloning, expression, and functional analysis of patient-derived hepatitis C virus glycoproteins.

Cross-genotype characterization of genetic diversity and molecular adaptation in hepatitis C virus envelope glycoprotein genes (2007)
Journal Article
Brown, R. J., Tarr, A. W., McClure, C. P., Juttla, V. S., Tagiuri, N., Irving, W. L., & Ball, J. K. (2007). Cross-genotype characterization of genetic diversity and molecular adaptation in hepatitis C virus envelope glycoprotein genes. Journal of General Virology, 88(2), 458-469. https://doi.org/10.1099/vir.0.82357-0

Investigation of the mechanisms underlying hepatitis C virus (HCV) envelope glycoprotein gene evolution will greatly assist rational development of broadly neutralizing antibody-based vaccines or vaccine components. Previously, comprehensive cross-ge... Read More about Cross-genotype characterization of genetic diversity and molecular adaptation in hepatitis C virus envelope glycoprotein genes.

Identification of Conserved Residues in the E2 Envelope Glycoprotein of the Hepatitis C Virus That Are Critical for CD81 Binding (2006)
Journal Article
Owsianka, A. M., Timms, J. M., Tarr, A. W., Brown, R. J., Hickling, T. P., Szwejk, A., Bienkowska-Szewczyk, K., Thomson, B. J., Patel, A. H., & Ball, J. K. (2006). Identification of Conserved Residues in the E2 Envelope Glycoprotein of the Hepatitis C Virus That Are Critical for CD81 Binding. Journal of Virology, 80(17), 8695-8704. https://doi.org/10.1128/JVI.00271-06

Hepatitis C virus (HCV) cell entry involves interaction between the viral envelope glycoprotein E2 and the cell surface receptor CD81. Knowledge of conserved E2 determinants important for successful binding will facilitate development of entry inhibi... Read More about Identification of Conserved Residues in the E2 Envelope Glycoprotein of the Hepatitis C Virus That Are Critical for CD81 Binding.

Characterization of the hepatitis C virus E2 epitope defined by the broadly neutralizing monoclonal antibody AP33 (2006)
Journal Article
Tarr, A. W., Owsianka, A. M., Timms, J. M., McClure, C. P., Brown, R. J., Hickling, T. P., Pietschmann, T., Bartenschlager, R., Patel, A. H., & Ball, J. K. (2006). Characterization of the hepatitis C virus E2 epitope defined by the broadly neutralizing monoclonal antibody AP33. Hepatology, 43(3), 592-601. https://doi.org/10.1002/hep.21088

The mouse monoclonal antibody (MAb) AP33, recognizing a 12 amino acid linear epitope in the hepatitis C virus (HCV) E2 glycoprotein, potently neutralizes retroviral pseudoparticles (HCVpp) carrying genetically diverse HCV envelope glycoproteins. Cons... Read More about Characterization of the hepatitis C virus E2 epitope defined by the broadly neutralizing monoclonal antibody AP33.

Monoclonal antibody AP33 defines a broadly neutralizing epitope on the hepatitis C virus E2 envelope glycoprotein (2005)
Journal Article
Owsianka, A., Tarr, A. W., Juttla, V. S., Lavillette, D., Bartosch, B., Cosset, F. L., Ball, J. K., & Patel, A. H. (2005). Monoclonal antibody AP33 defines a broadly neutralizing epitope on the hepatitis C virus E2 envelope glycoprotein. Journal of Virology, 79(17), 11095-11104. https://doi.org/10.1128/JVI.79.17.11095-11104.2005

Hepatitis C virus (HCV) remains a significant threat to the general health of the world's population, and there is a pressing need for the development of new treatments and preventative vaccines. Here, we describe the generation of retrovirus-based p... Read More about Monoclonal antibody AP33 defines a broadly neutralizing epitope on the hepatitis C virus E2 envelope glycoprotein.

Analysis of the binding of hepatitis C virus genotype 1a and 1b E2 glycoproteins to peripheral blood mononuclear cell subsets (2005)
Journal Article
Yamada, E., Montoya, M., Schuettler, C. G., Hickling, T. P., Tarr, A. W., Vitelli, A., Dubuisson, J., Patel, A. H., Ball, J. K., & Borrow, P. (2005). Analysis of the binding of hepatitis C virus genotype 1a and 1b E2 glycoproteins to peripheral blood mononuclear cell subsets. Journal of General Virology, 86(9), 2507-2512. https://doi.org/10.1099/vir.0.81169-0

Hepatitis C virus (HCV) binding to hepatocytes is thought to be mediated via interaction of the E2 glycoprotein with (co-)receptors including CD81 and scavenger receptor class B type I (SR-BI). Here, the expression of CD81 and SR-BI was analysed on p... Read More about Analysis of the binding of hepatitis C virus genotype 1a and 1b E2 glycoproteins to peripheral blood mononuclear cell subsets.

Evolutionary dynamics of hepatitis C virus envelope genes during chronic infection (2005)
Journal Article
Brown, R. J., Juttla, V. S., Tarr, A. W., Finnis, R., Irving, W. L., Hemsley, S., Flower, D. R., Borrow, P., & Ball, J. K. (2005). Evolutionary dynamics of hepatitis C virus envelope genes during chronic infection. Journal of General Virology, 86(7), 1931-1942. https://doi.org/10.1099/vir.0.80957-0

Hepatitis C virus (HCV) envelope glycoproteins E1 and E2 are important targets for the host immune response. The genes encoding these proteins exhibit a high degree of variability that gives rise to differing phenotypic traits, including alterations... Read More about Evolutionary dynamics of hepatitis C virus envelope genes during chronic infection.

Characterization of host-range and cell entry properties of the major genotypes and subtypes of hepatitis C virus (2005)
Journal Article
Lavillette, D., Tarr, A. W., Voisset, C., Donot, P., Bartosch, B., Bain, C., Patel, A. H., Dubuisson, J., Ball, J. K., & Cosset, F. L. (2005). Characterization of host-range and cell entry properties of the major genotypes and subtypes of hepatitis C virus. Hepatology, 41(2), 265-274. https://doi.org/10.1002/hep.20542

Because of the lack of a robust cell culture system, relatively little is known about the molecular details of the cell entry mechanism for hepatitis C virus (HCV). Recently, we described infectious HCV pseudo-particles (HCVpp) that were generated by... Read More about Characterization of host-range and cell entry properties of the major genotypes and subtypes of hepatitis C virus.

Tagged polymerase chain reaction subtractive hybridization for the enrichment of phage display random peptide libraries (2005)
Journal Article
Tarr, A. W., Boneham, S. P., Grabowska, A. M., & Ball, J. K. (2005). Tagged polymerase chain reaction subtractive hybridization for the enrichment of phage display random peptide libraries. Analytical Biochemistry, 339(1), 61-68. https://doi.org/10.1016/j.ab.2004.12.020

Affinity selection of phage display peptide libraries is routinely used for isolating peptides capable of binding a range of molecules, including antibodies and receptors. This process is most successful when the selecting molecule is relatively pure... Read More about Tagged polymerase chain reaction subtractive hybridization for the enrichment of phage display random peptide libraries.

Troubleshooting methods for the generation of novel pseudotyped viruses
Journal Article
King, B., Temperton, N., Grehan, K., Scott, S., Wright, E., Tarr, A. W., & Daly, J. M. Troubleshooting methods for the generation of novel pseudotyped viruses. Future Virology,

A pseudotyped virus (PV) is a virus particle with an envelope protein originating from a different virus. The ability to dictate which envelope proteins are expressed on the surface has made pseudotyping an important tool for basic virological studie... Read More about Troubleshooting methods for the generation of novel pseudotyped viruses.