Eriko Yamada
Analysis of the binding of hepatitis C virus genotype 1a and 1b E2 glycoproteins to peripheral blood mononuclear cell subsets
Yamada, Eriko; Montoya, Maria; Schuettler, Christian G.; Hickling, Timothy P.; Tarr, Alexander W.; Vitelli, Alessandra; Dubuisson, Jean; Patel, Arvind H.; Ball, Jonathan K.; Borrow, Persephone
Authors
Maria Montoya
Christian G. Schuettler
Timothy P. Hickling
Dr Alexander Tarr Alex.Tarr@nottingham.ac.uk
ASSOCIATE PROFESSOR
Alessandra Vitelli
Jean Dubuisson
Arvind H. Patel
Jonathan K. Ball
Persephone Borrow
Abstract
Hepatitis C virus (HCV) binding to hepatocytes is thought to be mediated via interaction of the E2 glycoprotein with (co-)receptors including CD81 and scavenger receptor class B type I (SR-BI). Here, the expression of CD81 and SR-BI was analysed on peripheral blood mononuclear cell (PBMC) subsets, and the binding of genotype 1 soluble truncated E2 (sE2) proteins to these cells was investigated. All PBMC subsets expressed CD81, although at varying levels. In contrast, SR-BI was only detected on monocytes and dendritic cells (DCs). The genotype 1a H77c sE2 protein showed higher PBMC binding than other genotype 1a/b sE2s. H77c sE2 binding to different PBMC subsets largely paralleled their level of CD81 expression, and could be inhibited by blocking E2-CD81 interaction. However, those PBMC subsets reported to be infected by HCV in vivo (monocytes, DCs and B cells) also exhibited residual, CD81-independent binding, indicating roles for SIR-BI/other receptor(s) in mediating haematopoietic cell infection. © 2005 SGM.
Citation
Yamada, E., Montoya, M., Schuettler, C. G., Hickling, T. P., Tarr, A. W., Vitelli, A., Dubuisson, J., Patel, A. H., Ball, J. K., & Borrow, P. (2005). Analysis of the binding of hepatitis C virus genotype 1a and 1b E2 glycoproteins to peripheral blood mononuclear cell subsets. Journal of General Virology, 86(9), 2507-2512. https://doi.org/10.1099/vir.0.81169-0
| Journal Article Type | Article |
|---|---|
| Online Publication Date | Sep 1, 2005 |
| Publication Date | Sep 1, 2005 |
| Deposit Date | Nov 9, 2022 |
| Journal | Journal of General Virology |
| Print ISSN | 0022-1317 |
| Electronic ISSN | 1465-2099 |
| Publisher | Microbiology Society |
| Peer Reviewed | Peer Reviewed |
| Volume | 86 |
| Issue | 9 |
| Pages | 2507-2512 |
| DOI | https://doi.org/10.1099/vir.0.81169-0 |
| Public URL | https://nottingham-repository.worktribe.com/output/3129665 |
| Publisher URL | https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.81169-0 |