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Condenser-free contrast methods for transmitted-light microscopy (2014)
Journal Article
Webb, K. F. (2014). Condenser-free contrast methods for transmitted-light microscopy. Journal of Microscopy, 257(1), 8-22. https://doi.org/10.1111/jmi.12181

Phase contrast microscopy allows the study of highly transparent yet detail-rich specimens by producing intensity contrast from phase objects within the sample. Presented here is a generalized phase contrast illumination schema in which condenser opt... Read More about Condenser-free contrast methods for transmitted-light microscopy.

Identification of a nonselective cation channel in isolated lens fiber cells that is activated by cell shrinkage (2012)
Journal Article
Gunning, S. J., Chung, K. K., Donaldson, P. J., & Webb, K. F. (2012). Identification of a nonselective cation channel in isolated lens fiber cells that is activated by cell shrinkage. American Journal of Physiology - Cell Physiology, 303(12), C1252-C1259. https://doi.org/10.1152/ajpcell.00237.2012

The initiation of lens cataract has long been associated with the development of a membrane "leak" in lens fiber cells that depolarizes the lens intracellular potential and elevates intracellular Na+ and Ca2+ concentrations. It has been proposed that... Read More about Identification of a nonselective cation channel in isolated lens fiber cells that is activated by cell shrinkage.

Regulation of lens volume: Implications for lens transparency (2009)
Journal Article
Donaldson, P. J., Chee, K. S. N., Lim, J. C., & Webb, K. F. (2009). Regulation of lens volume: Implications for lens transparency. Experimental Eye Research, 88(2), 144-150. https://doi.org/10.1016/j.exer.2008.05.011

Lens transparency is critically dependent on the maintenance of an ordered tissue architecture, and disruption of this order leads to light scatter and eventually lens cataract. Hence the volume of the fiber cells that make up the bulk of the lens ne... Read More about Regulation of lens volume: Implications for lens transparency.

Molecular and functional mapping of regional differences in P2Y receptor expression in the rat lens (2008)
Journal Article
Hu, R. G., Suzuki-Kerr, H., Webb, K. F., Rhodes, J. D., Collison, D. J., Duncan, G., & Donaldson, P. J. (2008). Molecular and functional mapping of regional differences in P2Y receptor expression in the rat lens. Experimental Eye Research, 87(2), 137-146. https://doi.org/10.1016/j.exer.2008.05.008

Extracellular ATP has been shown to mobilize intracellular Ca2+ in cultured ovine lens epithelial cells and in human lens epithelium, suggesting a role for purines in the modulation of lens transparency. In this study, we characterized the expression... Read More about Molecular and functional mapping of regional differences in P2Y receptor expression in the rat lens.