All Outputs (2)

Design, Construction, and Functional Characterization of a tRNA Neochromosome in Yeast (2022)
Preprint / Working Paper
Schindler, D., Walker, R. S., Jiang, S., Brooks, A. N., Wang, Y., Müller, C. A., Cockram, C., Luo, Y., García, A., Schraivogel, D., Mozziconacci, J., Blount, B. A., Cai, J., Ogunlana, L., Liu, W., Jönsson, K., Abramczyk, D., Garcia-Ruiz, E., Turowski, T. W., Swidah, R., …Cai, Y. Design, Construction, and Functional Characterization of a tRNA Neochromosome in Yeast

Here we report the design, construction and characterization of a tRNA neochromosome, a designer chromosome that functions as an additional, de novo counterpart to the native complement of Saccharomyces cerevisiae. Intending to address one of the cen... Read More about Design, Construction, and Functional Characterization of a tRNA Neochromosome in Yeast.

Synthetic yeast chromosome XI design enables extrachromosomal circular DNA formation on demand (2022)
Preprint / Working Paper
Blount, B. A., Lu, X., Driessen, M. R. M., Jovicevic, D., Sanchez, M. I., Ciurkot, K., Zhao, Y., Lauer, S., McKiernan, R. M., Gowers, G.-O. F., Sweeney, F., Fanfani, V., Lobzaev, E., Palacios-Flores, K., Walker, R., Hesketh, A., Oliver, S. G., Cai, Y., Stracquadanio, G., Mitchell, L. A., …Ellis, T. Synthetic yeast chromosome XI design enables extrachromosomal circular DNA formation on demand

We describe construction of the 660 kilobase synthetic yeast chromosome XI (synXI) and reveal how synthetic redesign of non-coding DNA elements impact the cell. To aid construction from synthesized 5 to 10 kilobase DNA fragments, we implemented CRISP... Read More about Synthetic yeast chromosome XI design enables extrachromosomal circular DNA formation on demand.