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Dietary supplementation of nucleotides and oligosaccharides in kittens reduces the expression of circulating miR13p, miR133a3p, miR2063p and miR3835p (2024)
Journal Article
Piccinini, A. M., Albanese, F., Harrison, M., Farquhar, M. J., Holcombe, L. J., Atwal, J., & Watson, P. (in press). Dietary supplementation of nucleotides and oligosaccharides in kittens reduces the expression of circulating miR13p, miR133a3p, miR2063p and miR3835p. Frontiers in Veterinary Science, https://doi.org/10.3389/fvets.2024.1382436

PLGA-PEG-PLGA hydrogels induce cytotoxicity in conventional in vitro assays (2024)
Journal Article
Stewart, C. L., Hook, A. L., Zelzer, M., Marlow, M., & Piccinini, A. M. (2024). PLGA-PEG-PLGA hydrogels induce cytotoxicity in conventional in vitro assays. Cell Biochemistry and Function, 42(5), Article e4097. https://doi.org/10.1002/cbf.4097

We identified that PLGA-PEG-PLGA hydrogels, which have been used in human clinical trials and possess a demonstrable safety profile, induced significant cytotoxicity in conventional in vitro assays. This major contradiction may lead to inconsistent a... Read More about PLGA-PEG-PLGA hydrogels induce cytotoxicity in conventional in vitro assays.

Cellular and microenvironmental cues that promote macrophage fusion and foreign body response (2024)
Journal Article
Stewart, C. L., Marlow, M., Zelzer, M., Hook, A. L., & Piccinini, A. M. (in press). Cellular and microenvironmental cues that promote macrophage fusion and foreign body response. Frontiers in Immunology, 15, https://doi.org/10.3389/fimmu.2024.1411872

During the foreign body response (FBR), macrophages fuse to form foreign body giant cells (FBGCs). Modulation of FBGC formation can prevent biomaterial degradation and loss of therapeutic efficacy. However, the microenvironmental cues that dictate FB... Read More about Cellular and microenvironmental cues that promote macrophage fusion and foreign body response.

A virological view of tenascin-C in infection (2022)
Journal Article
Zuliani-Alvarez, L., & Piccinini, A. M. (2023). A virological view of tenascin-C in infection. American Journal of Physiology - Cell Physiology, 324(1), C1-C9. https://doi.org/10.1152/ajpcell.00333.2022

Tenascin-C is a large extracellular matrix glycoprotein with complex, not yet fully unveiled roles. Its context- and structure-dependent modus operandi renders tenascin-C a puzzling protein. Since its discovery ∼40 years ago, research into tenascin-C... Read More about A virological view of tenascin-C in infection.

miR-155-3p: processing by-product or rising star in immunity and cancer? (2022)
Journal Article
Dawson, O., & Piccinini, A. M. (2022). miR-155-3p: processing by-product or rising star in immunity and cancer?. Open Biology, 12(5), 843-854. https://doi.org/10.1098/rsob.220070

MicroRNAs (miRNAs) are key players in gene regulation that target specific mRNAs for degradation or translational repression. Each miRNA is synthesized as a miRNA duplex comprising two strands (5p and 3p). However, only one of the two strands becomes... Read More about miR-155-3p: processing by-product or rising star in immunity and cancer?.

Therapeutic Effects of Hypoxic and Pro-Inflammatory Priming of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Inflammatory Arthritis (2021)
Journal Article
Kay, A. G., Treadwell, K., Roach, P., Morgan, R., Lodge, R., Hyland, M., Piccinini, A. M., Forsyth, N. R., & Kehoe, O. (2022). Therapeutic Effects of Hypoxic and Pro-Inflammatory Priming of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Inflammatory Arthritis. International Journal of Molecular Sciences, 23(1), Article 126. https://doi.org/10.3390/ijms23010126

Mesenchymal stem cells (MSCs) immunomodulate inflammatory responses through paracrine signalling, including via secretion of extracellular vesicles (EVs) in the cell secretome. We evaluated the therapeutic potential of MSCs-derived small EVs in an an... Read More about Therapeutic Effects of Hypoxic and Pro-Inflammatory Priming of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Inflammatory Arthritis.

Gestational poly(I:C) attenuates, not exacerbates, the behavioral, cytokine and mTOR changes caused by isolation rearing in a rat ‘dual-hit’ model for neurodevelopmental disorders (2020)
Journal Article
Goh, J. Y., O'Sullivan, S. E., Shortall, S. E., Zordan, N., Piccinini, A. M., Potter, H. G., Fone, K. C., & King, M. V. (2020). Gestational poly(I:C) attenuates, not exacerbates, the behavioral, cytokine and mTOR changes caused by isolation rearing in a rat ‘dual-hit’ model for neurodevelopmental disorders. Brain, Behavior, and Immunity, 89, 100-117. https://doi.org/10.1016/j.bbi.2020.05.076

© 2020 Elsevier Inc. Many psychiatric illnesses have a multifactorial etiology involving genetic and environmental risk factors that trigger persistent neurodevelopmental impairments. Several risk factors have been individually replicated in rodents,... Read More about Gestational poly(I:C) attenuates, not exacerbates, the behavioral, cytokine and mTOR changes caused by isolation rearing in a rat ‘dual-hit’ model for neurodevelopmental disorders.

The polyadenylation inhibitor cordycepin reduces pain, inflammation and joint pathology in rodent models of osteoarthritis (2019)
Journal Article
Ashraf, S., Radhi, M., Gowler, P., Burston, J. J., Gandhi, R. D., Thorn, G. J., Piccinini, A. M., Walsh, D. A., Chapman, V., De Moor, C. H., & Chapman, V. (2019). The polyadenylation inhibitor cordycepin reduces pain, inflammation and joint pathology in rodent models of osteoarthritis. Scientific Reports, 9(1), 1-17. https://doi.org/10.1038/s41598-019-41140-1

Clinically, osteoarthritis (OA) pain is significantly associated with synovial inflammation. Identification of the mechanisms driving inflammation could reveal new targets to relieve this prevalent pain state. Herein, a role of polyadenylation in OA... Read More about The polyadenylation inhibitor cordycepin reduces pain, inflammation and joint pathology in rodent models of osteoarthritis.

A complex interplay between the extracellular matrix and the innate immune response to microbial pathogens (2018)
Journal Article
Tomlin, H., & Piccinini, A. M. (2018). A complex interplay between the extracellular matrix and the innate immune response to microbial pathogens. Immunology, 155(2), 186-201. https://doi.org/10.1111/imm.12972

The role of the host extracellular matrix (ECM) in infection tends to be neglected. However, the complex interactions between invading pathogens, host tissues and immune cells occur in the context of the ECM. On the pathogen side, a variety of surfac... Read More about A complex interplay between the extracellular matrix and the innate immune response to microbial pathogens.

Mesenchymal stem cell-conditioned medium reduces disease severity and immune responses in inflammatory arthritis (2017)
Journal Article
Kay, A. G., Long, G., Tyler, G., Stefan, A., Broadfoot, S. J., Piccinini, A. M., Middleton, J., & Kehoe, O. (2017). Mesenchymal stem cell-conditioned medium reduces disease severity and immune responses in inflammatory arthritis. Scientific Reports, 7, Article 18019. https://doi.org/10.1038/s41598-017-18144-w

We evaluated the therapeutic potential of mesenchymal stem cell-conditioned medium (CM-MSC) as an alternative to cell therapy in an antigen-induced model of arthritis (AIA). Disease severity and cartilage loss were evaluated by histopathological anal... Read More about Mesenchymal stem cell-conditioned medium reduces disease severity and immune responses in inflammatory arthritis.

Mapping tenascin-C interaction with toll-like receptor 4 reveals a new subset of endogenous inflammatory triggers (2017)
Journal Article
Zuliani-Alvarez, L., Marzeda, A. M., Deligne, C., Schwenzer, A., McCann, F. E., Marsden, B. D., Piccinini, A. M., & Midwood, K. S. (2017). Mapping tenascin-C interaction with toll-like receptor 4 reveals a new subset of endogenous inflammatory triggers. Nature Communications, 8(1), Article 1595. https://doi.org/10.1038/s41467-017-01718-7

© 2017 The Author(s). Pattern recognition underpins innate immunity; the accurate identification of danger, including infection, injury, or tumor, is key to an appropriately targeted immune response. Pathogen detection is increasingly well defined me... Read More about Mapping tenascin-C interaction with toll-like receptor 4 reveals a new subset of endogenous inflammatory triggers.

Screening for novel endogenous inflammatory stimuli using the secreted embryonic alkaline phosphatase NF-κB reporter assay (2017)
Journal Article
Zuliani-Alvarez, L., Piccinini, A. M., & Midwood, K. S. (2017). Screening for novel endogenous inflammatory stimuli using the secreted embryonic alkaline phosphatase NF-κB reporter assay. Bio-protocol, 7(7), https://doi.org/10.21769/BioProtoc.2220

An immune response can be activated by pathogenic stimuli, as well as endogenous danger signals, triggering the activation of pattern recognition receptors and initiating signalling cascades that lead to inflammation. This method uses THP1-Blue™ cell... Read More about Screening for novel endogenous inflammatory stimuli using the secreted embryonic alkaline phosphatase NF-κB reporter assay.

Distinct microenvironmental cues stimulate divergent TLR4-mediated signaling pathways in macrophages (2016)
Journal Article
Piccinini, A. M., Zuliani-Alvarez, L., Lim, J. M., & Midwood, K. S. (2016). Distinct microenvironmental cues stimulate divergent TLR4-mediated signaling pathways in macrophages. Science Signaling, 9(443), ra86-ra86. https://doi.org/10.1126/scisignal.aaf3596

Macrophages exhibit a phenotypic plasticity that enables them to orchestrate specific immune responses to distinct threats. However, the factors that control macrophage behaviour in a context dependent manner are not well understood. Lipopolysacchari... Read More about Distinct microenvironmental cues stimulate divergent TLR4-mediated signaling pathways in macrophages.

Interfering with the CCL2-glycosaminoglycan axis as a potential approach to modulate neuroinflammation (2016)
Journal Article
Gschwandtner, M., Piccinini, A. M., Geriza, T., Adage, T., & Kungl, A. (2016). Interfering with the CCL2-glycosaminoglycan axis as a potential approach to modulate neuroinflammation. Neuroscience Letters, 626, 164-173. https://doi.org/10.1016/j.neulet.2016.05.037

© 2016 Elsevier Ireland Ltd. Multiple Sclerosis, a chronic inflammatory demyelinating disease of the central nervous system, involves an increased expression of monocyte chemotactic protein 1 MCP1-/CCL2. For exerting its chemotactic effects, chemokin... Read More about Interfering with the CCL2-glycosaminoglycan axis as a potential approach to modulate neuroinflammation.

Investigating the role of toll-like receptors in models of arthritis (2016)
Journal Article
Piccinini, A. M., Williams, L., McCann, F. E., & Midwood, K. S. (2016). Investigating the role of toll-like receptors in models of arthritis. Methods in Molecular Biology, 1390, 351-381. https://doi.org/10.1007/978-1-4939-3335-8_22

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by persistent synovial inflammation leading to tissue destruction and progressive loss of joint function. Here we describe two methods that can be used to assess the contribution... Read More about Investigating the role of toll-like receptors in models of arthritis.

Illustrating the interplay between the extracellular matrix and microRNAs (2014)
Journal Article
Piccinini, A. M., & Midwood, K. S. (2014). Illustrating the interplay between the extracellular matrix and microRNAs. International Journal of Experimental Pathology, 95(3), 158-180. https://doi.org/10.1111/iep.12079

The discovery of cell surface receptors that bind to extracellular matrix (ECM) components marked a new era in biological research. Since then there has been an increasing appreciation of the importance of studying cells in the context of their extra... Read More about Illustrating the interplay between the extracellular matrix and microRNAs.