Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate

Giri, Jitender; Bhosale, Rahul; Huang, Guoqiang; Pandey, Bipin K.; Parker, Helen; Zappala, Susan; Yang, Jing; Dievart, Anne; Bureau, Charlotte; Ljung, Karin; Price, Adam; Rose, Terry; Larrieu, Antoine; Mairhofer, Stefan; Sturrock, Craig J.; White, Philip; Dupuy, Lionel; Hawkesford, Malcolm; Perin, Christophe; Liang, Wanqi; Peret, Benjamin; Hodgman, Charlie T.; Lynch, Jonathan; Wissuwa, Matthias; Zhang, Dabing; Pridmore, Tony; Mooney, Sacha J.; Guiderdoni, Emmanuel; Swarup, Ranjan; Bennett, Malcolm J.

Abstract

Root traits such as root angle and hair length influence resource acquisition particularly for immobile nutrients like phosphorus (P). Here, we attempted to modify root angle in rice by disrupting the OsAUX1 auxin influx transporter gene in an effort to improve rice P acquisition efficiency. We show by X-ray microCT imaging that root angle is altered in the osaux1 mutant, causing preferential foraging in the top soil where P normally accumulates, yet surprisingly, P acquisition efficiency does not improve. Through closer investigation, we reveal that OsAUX1 also promotes root hair elongation in response to P limitation. Reporter studies reveal that auxin response increases in the root hair zone in low P environments. We demonstrate that OsAUX1 functions to mobilize auxin from the root apex to the differentiation zone where this signal promotes hair elongation when roots encounter low external P. We conclude that auxin and OsAUX1 play key roles in promoting root foraging for P in rice.

Citation

Giri, J., Bhosale, R., Huang, G., Pandey, B. K., Parker, H., Zappala, S., Yang, J., Dievart, A., Bureau, C., Ljung, K., Price, A., Rose, T., Larrieu, A., Mairhofer, S., Sturrock, C. J., White, P., Dupuy, L., Hawkesford, M., Perin, C., Liang, W., …Bennett, M. J. (2018). Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate. Nature Communications, 9(1), https://doi.org/10.1038/s41467-018-03850-4

• Journal Article Type: Article
• Acceptance Date: Mar 16, 2018
• Online Publication Date: Apr 12, 2018
• Publication Date: Dec 1, 2018
• Deposit Date: Apr 12, 2018
• Publicly Available Date: Apr 12, 2018
• Journal: Nature Communications
• Electronic ISSN: 2041-1723
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 9
• Issue: 1
• DOI: https://doi.org/10.1038/s41467-018-03850-4
• Public URL: https://nottingham-repository.worktribe.com/output/924833
• Publisher URL: https://www.nature.com/articles/s41467-018-03850-4
• Additional Information: Received: 9 January 2017; Accepted: 16 March 2018; First Online: 12 April 2018; Change Date: 1 May 2018; Change Type: Correction; Change Details: The original version of this Article omitted the following from the Acknowledgements:‘We also thank DBT-CREST BT/HRD/03/01/2002.’This has been corrected in both the PDF and HTML versions of the Article.; : The authors declare no competing interests.
• Contract Date: Apr 12, 2018