Kai Betteridge
Sialic acids regulate microvessel permeability, revealed by novel in vivo studies of endothelial glycocalyx structure and function
Betteridge, Kai; Arkill, Kenton; Neal, Chris; Harper, Steve; Foster, Becky; Satchell, Simon; Bates, David O.; Salmon, Andy
Authors
KENTON ARKILL Kenton.Arkill@nottingham.ac.uk
Associate Professor
Chris Neal
Steve Harper
Becky Foster
Simon Satchell
DAVID BATES David.Bates@nottingham.ac.uk
Professor of Oncology
Andy Salmon
Abstract
The endothelial glycocalyx forms a continuous coat over the luminal surface of all vessels, and regulates multiple vascular functions. The contribution of individual components of the endothelial glycocalyx to one critical vascular function, microvascular permeability, remains unclear. We developed novel, real time, paired methodologies to study the contribution of sialic acids within the endothelial glycocalyx to the structural and functional permeability properties of the same microvessel in vivo. Single perfused rat mesenteric microvessels were perfused with fluorescent endothelial cell membrane and glycocalyx labels, and imaged with confocal microscopy. A broad range of glycocalyx depth measurements (0.17–3.02μm) were obtained with different labels, imaging techniques and analysis methods. The distance between peak cell membrane and peak glycocalyx label provided the most reliable measure of endothelial glycocalyx anatomy, correlating with paired, numerically smaller values of endothelial glycocalyx depth (0.078±0.016μm) from electron micrographs of the same portion of the same vessel. Disruption of sialic acid residues within the endothelial glycocalyx using neuraminidase perfusion decreased endothelial glycocalyx depth and increased apparent solute permeability to albumin in the same vessels in a timedependent manner, with changes in all three true vessel wall permeability coefficients (hydraulic conductivity, reflection coefficient, and diffusive solute permeability). These novel technologies expand the range of techniques that permit direct studies of the structure of the endothelial glycocalyx and dependent microvascular functions in vivo, and demonstrate that sialic acid residues within the endothelial glycocalyx are critical regulators of microvascular permeability to both water and albumin.
Citation
Betteridge, K., Arkill, K., Neal, C., Harper, S., Foster, B., Satchell, S., …Salmon, A. (2017). Sialic acids regulate microvessel permeability, revealed by novel in vivo studies of endothelial glycocalyx structure and function. Journal of Physiology, 595(15), https://doi.org/10.1113/JP274167
Journal Article Type | Article |
---|---|
Acceptance Date | May 8, 2017 |
Online Publication Date | May 19, 2017 |
Publication Date | Aug 1, 2017 |
Deposit Date | May 24, 2017 |
Publicly Available Date | May 24, 2017 |
Journal | Journal of Physiology |
Print ISSN | 0022-3751 |
Electronic ISSN | 1469-7793 |
Publisher | Wiley |
Peer Reviewed | Peer Reviewed |
Volume | 595 |
Issue | 15 |
DOI | https://doi.org/10.1113/JP274167 |
Public URL | https://nottingham-repository.worktribe.com/output/875547 |
Publisher URL | http://onlinelibrary.wiley.com/doi/10.1113/JP274167/abstract |
Contract Date | May 24, 2017 |
Files
Betteridge_et_al-2017-The_Journal_of_Physiology.pdf
(1.9 Mb)
PDF
Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
Endothelial Glycocalyx Sialic Acids Regulate Microvessel Permeability - revised complete text.pdf
(1.7 Mb)
PDF
Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
You might also like
Transformations of citrate and Tween coated silver nanoparticles reacted with Na2S
(2014)
Journal Article
Model-based image analysis of a tethered Brownian fibre for shear stress sensing
(2017)
Journal Article
Downloadable Citations
About Repository@Nottingham
Administrator e-mail: discovery-access-systems@nottingham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search