The CEP5 peptide promotes abiotic stress tolerance, as revealed by quantitative proteomics, and attenuates the AUX/IAA equilibrium in Arabidopsis

Smith, Stephanie; Zhu, Shanshuo; Joos, Lisa; Roberts, Ianto; Nikonorova, Natalia; Vu, Lam Dai; Stes, Elisabeth; Cho, Hyunwoo; Larrieu, Antoine; Xuan, Wei; Goodall, Benjamin; Cotte, Brigitte van de; Waite, Jessic Marie; Rigal, Adeline; Harborough, Sigurd R.R.; Persiau, Geert; Vanneste, Steffen; Kirschner, Gwendolyn K.; Vandermarliere, Elien; Martens, Lennart; Stahl, Yvonne; Audenaert, Dominique; Friml, Jiri; Felix, Georg; Simon, Ruediger; Bennett, Malcolm; Bishopp, Anthony; De Jaeger, Geert; Ljung, Karin; Kepinski, Stefan; Robert, Stephanie; Nemhauser, Jennifer; Hwang, Ildoo; Gevaert, Kris; Beeckman, Tom; De Smet, Ive

Abstract

Peptides derived from non-functional precursors play important roles in various developmental processes, but also in (a)biotic stress signaling. Our (phospho)proteome-wide analyses of C-terminally encoded peptide 5 (CEP5)-mediated changes revealed an impact on abiotic stress-related processes. Drought has a dramatic impact on plant growth, development and reproduction, and the plant hormone auxin plays a role in drought responses. Our genetic, physiological, biochemical and pharmacological results demonstrated that CEP5-mediated signaling is relevant for osmotic and drought stress tolerance in Arabidopsis, and that CEP5 specifically counteracts auxin effects. Specifically, we found that CEP5 signaling stabilizes AUX/IAA transcriptional repressors, suggesting the existence of a novel peptide-dependent control mechanism that tunes auxin signaling. These observations align with the recently described role of AUX/IAAs in stress tolerance and provide a novel role for CEP5 in osmotic and drought stress tolerance.

Citation

Smith, S., Zhu, S., Joos, L., Roberts, I., Nikonorova, N., Vu, L. D., Stes, E., Cho, H., Larrieu, A., Xuan, W., Goodall, B., Cotte, B. V. D., Waite, J. M., Rigal, A., Harborough, S. R., Persiau, G., Vanneste, S., Kirschner, G. K., Vandermarliere, E., Martens, L., …De Smet, I. (2020). The CEP5 peptide promotes abiotic stress tolerance, as revealed by quantitative proteomics, and attenuates the AUX/IAA equilibrium in Arabidopsis. Molecular and Cellular Proteomics, 19(8), 1248-1262. https://doi.org/10.1074/mcp.ra119.001826

• Journal Article Type: Article
• Acceptance Date: May 13, 2020
• Online Publication Date: May 13, 2020
• Publication Date: Aug 1, 2020
• Deposit Date: May 18, 2020
• Publicly Available Date: May 19, 2020
• Journal: Molecular and Cellular Proteomics
• Print ISSN: 1535-9476
• Electronic ISSN: 1535-9484
• Publisher: American Society for Biochemistry and Molecular Biology
• Peer Reviewed: Peer Reviewed
• Volume: 19
• Issue: 8
• Pages: 1248-1262
• DOI: https://doi.org/10.1074/mcp.ra119.001826
• Keywords: Analytical Chemistry; Biochemistry; Molecular Biology
• Public URL: https://nottingham-repository.worktribe.com/output/4463167
• Publisher URL: https://www.mcponline.org/content/early/2020/05/13/mcp.RA119.001826
• Additional Information: This research was originally published in the Journal of Biological Chemistry. Stephanie Smith, Shanshuo Zhu, Lisa Joos, Ianto Roberts, Natalia Nikonorova, Lam Dai Vu, Elisabeth Stes, Hyunwoo Cho, Antoine Larrieu, Wei Xuan, Benjamin Goodall, Brigitte van de Cotte, Jessic Marie Waite, Adeline Rigal, Sigurd R. R. Harborough, Geert Persiau, Steffen Vanneste, Gwendolyn K. Kirschner, Elien Vandermarliere, Lennart Martens, Yvonne Stahl, Dominique Audenaert, Jiri Friml, Georg Felix, Ruediger Simon, Malcolm Bennett, Anthony Bishopp, Geert De Jaeger, Karin Ljung, Stefan Kepinski, Stephanie Robert, Jennifer Nemhauser, Ildoo Hwang, Kris Gevaert, Tom Beeckman, Ive De Smet. The CEP5 peptide promotes abiotic stress tolerance, as revealed by quantitative proteomics, and attenuates the AUX/IAA equilibrium in Arabidopsis. J Biol Chem. Year; Vol:pp-pp. © the American Society for Biochemistry and Molecular Biology or © the Author(s).