Prognostic Significance of Nucleolar Assessment in Invasive Breast Cancer

Elsharawy, Khloud A.; Toss, Michael S.; Raaft, Sara R.; Ball, Graham; Green, Andrew R.; Aleskandarany, Mohammed A.; Dalton, Leslie W.; Rakha, Emad A.

Abstract

Aims: Nucleolar morphometric features have a potential role in the assessment of aggressiveness of many cancers. However, the role of nucleoli in invasive breast cancer (IBC) is still unclear. This study aimed to investigate the optimal scoring method of nucleoli in IBC and their prognostic significance, and refine the grading of BC by incorporating the nucleolar score.
Methods and results: Digital images acquired from hematoxylin and eosin (H&E) stained sections from a large IBC cohort were divided into training (n=400) and validation (n=1200) sets were used in this study. Four different assessment methods including 1) modified Helpap’s method, and counting prominent nucleoli (size ≥2.5μm) in 2) 10 field views (10 FVs), 3) 5 FVs and 4) 1 FV were evaluated in the training set to identify the optimal method associated with the best performance and significant prognostic value. The optimal method was applied to the validation set and to an external validation set the Cancer Genome Atlas (TCGA) data (n=743). Scoring prominent nucleoli in 5 FVs, showed the highest inter-observer concordance rate (intraclass correlation coefficient = 0.8) and significant association with breast cancer specific survival (BCSS) (p

Citation

Elsharawy, K. A., Toss, M. S., Raaft, S. R., Ball, G., Green, A. R., Aleskandarany, M. A., Dalton, L. W., & Rakha, E. A. (2020). Prognostic Significance of Nucleolar Assessment in Invasive Breast Cancer. Histopathology, 76(5), 671-684. https://doi.org/10.1111/his.14036

• Journal Article Type: Article
• Acceptance Date: Nov 14, 2019
• Online Publication Date: Nov 17, 2019
• Publication Date: 2020-04
• Deposit Date: Nov 18, 2019
• Publicly Available Date: Nov 18, 2020
• Journal: Histopathology
• Print ISSN: 0309-0167
• Electronic ISSN: 1365-2559
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 76
• Issue: 5
• Pages: 671-684
• DOI: https://doi.org/10.1111/his.14036
• Keywords: Nucleoli, invasive breast cancer, methods, prognosis
• Public URL: https://nottingham-repository.worktribe.com/output/3323962
• Publisher URL: https://onlinelibrary.wiley.com/doi/10.1111/his.14036
• Additional Information: This is the peer reviewed version of the following article: Elsharawy, KA, Toss, MS, Abuelmaaty, SR, Ball, G, Green, AR, Aleskandarany, MA, Dalton, LW & Rakha, EA. ( 2019) Histopathology. https://doi.org/10.1111/his.14036 Prognostic Significance of Nucleolar Assessment in Invasive Breast Cancer, which has been published in final form at https://doi.org/10.1111/his.14036. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
• Contract Date: Nov 18, 2019