Root development is maintained by specific bacteria-bacteria interactions within a complex microbiome

Finkel, Omri M.; Salas-González, Isai; Castrillo, Gabriel; Law, Theresa F.; Conway, Jonathan M.; Lima Teixeira, Paulo José Pereira; Jones, Corbin D.; Dangl, Jeffery L.

Abstract

Plants grow within a complex web of species interacting with each other and with the plant via a wide repertoire of chemical signals. To model plant-microbe-microbe-environment interactions, we inoculated seedlings with a defined 185-member bacterial synthetic community (SynCom), and manipulated the abiotic environment to enable classification of the SynCom to modules of co-occurring strains. We deconstructed the SynCom based on these modules, identifying a single bacterial genus, Variovorax, which reverts phenotypic effects on root development induced by a wide diversity of bacterial strains and by the entire 185-member community. Variovorax use mechanisms related to auxin and ethylene manipulation to balance this ecologically realistic root community’s effects on root development. We demonstrate metabolic signal interference within a complex model community, defining Variovorax as determinants of bacteria-plant communication networks.

Citation

Finkel, O. M., Salas-González, I., Castrillo, G., Law, T. F., Conway, J. M., Lima Teixeira, P. J. P., Jones, C. D., & Dangl, J. L. Root development is maintained by specific bacteria-bacteria interactions within a complex microbiome

• Other Type: Other
• Deposit Date: Jul 24, 2019
• Peer Reviewed: Not Peer Reviewed
• DOI: https://doi.org/10.1101/645655
• Public URL: https://nottingham-repository.worktribe.com/output/2338273