A nuclear magnetic resonance study of water in aggrecan solutions

Foster, Richard J.; Damion, Robin A.; Baboolal, Thomas G.; Smye, Stephen W.; Ries, Michael E.

Abstract

Aggrecan, a highly charged macromolecule found in articular cartilage, was investigated in aqueous salt solutions with proton nuclear magnetic resonance. The longitudinal and transverse relaxation rates were determined at two different field strengths, 9.4 T and 0.5 T, for a range of temperatures and aggrecan concentrations. The diffusion coefficients of the water molecules were also measured as a function of temperature and aggrecan concentration, using a pulsed field gradient technique at 9.4 T. Assuming an Arrhenius relationship, the activation energies for the various relaxation processes and the translational motion of the water molecules were determined from temperature dependencies as a function of aggrecan concentration in the range 0–5.3% w/w. The longitudinal relaxation rate and inverse diffusion coefficient were approximately equally dependent on concentration and only increased by upto 20% from that of the salt solution. The transverse relaxation rate at high field demonstrated greatest concentration dependence, changing by an order of magnitude across the concentration range examined. We attribute this primarily to chemical exchange. Activation energies appeared to be approximately independent of aggrecan concentration, except for that of the low-field transverse relaxation rate, which decreased with concentration.

Citation

Foster, R. J., Damion, R. A., Baboolal, T. G., Smye, S. W., & Ries, M. E. (2016). A nuclear magnetic resonance study of water in aggrecan solutions. Royal Society Open Science, 3(3), https://doi.org/10.1098/rsos.150705

• Journal Article Type: Article
• Acceptance Date: Feb 12, 2016
• Online Publication Date: Mar 1, 2016
• Publication Date: Mar 1, 2016
• Deposit Date: Dec 5, 2022
• Publicly Available Date: Dec 5, 2022
• Journal: Royal Society Open Science
• Electronic ISSN: 2054-5703
• Publisher: The Royal Society
• Peer Reviewed: Peer Reviewed
• Volume: 3
• Issue: 3
• DOI: https://doi.org/10.1098/rsos.150705
• Public URL: https://nottingham-repository.worktribe.com/output/14321103
• Publisher URL: https://royalsocietypublishing.org/doi/10.1098/rsos.150705