Outputs (40)

Saliva for COVID-19 testing: Simple but useless or an undervalued resource? (2021)
Journal Article
Pijuan-Galito, S., Tarantini, F. S., Tomlin, H., Jenkins, H., Thompson, J. L., Scales, D., Stroud, A., Tellechea Lopez, A., Hassall, J., McTernan, P., Coultas, A., Arendt-Tranholm, A., Reffin, C., Hill, I., Lee, I.-N., Wu, S., Porte, J., Chappell, J., Lis-Slimak, K., Kaneko, K., …Denning, C. (2021). Saliva for COVID-19 testing: Simple but useless or an undervalued resource?. Frontiers in Virology, 1, Article 778790. https://doi.org/10.3389/fviro.2021.778790

During the COVID-19 pandemic, countries with robust population-based asymptomatic testing were generally successful in controlling virus spread, hence reducing hospitalizations and deaths. This effectiveness inspired widespread asymptomatic surveilla... Read More about Saliva for COVID-19 testing: Simple but useless or an undervalued resource?.

Transcriptomic Analysis of Cardiomyocyte Extracellular Vesicles in Hypertrophic Cardiomyopathy Reveals Differential snoRNA Cargo (2021)
Journal Article
James, V., Nizamudeen, Z. A., Lea, D., Dottorini, T., Holmes, T. L., Johnson, B. B., Arkill, K. P., Denning, C., & Smith, J. G. (2021). Transcriptomic Analysis of Cardiomyocyte Extracellular Vesicles in Hypertrophic Cardiomyopathy Reveals Differential snoRNA Cargo. Stem Cells and Development, 30(24), 1215-1227. https://doi.org/10.1089/scd.2021.0202

Hypertrophic cardiomyopathy (HCM) is characterised by increased left ventricular wall thickness that can lead to devastating conditions such as heart failure and sudden cardiac death. Despite extensive study, the mechanisms mediating many of the asso... Read More about Transcriptomic Analysis of Cardiomyocyte Extracellular Vesicles in Hypertrophic Cardiomyopathy Reveals Differential snoRNA Cargo.

Impacts of the COVID-19 pandemic and self-isolation on students and staff in higher education: A qualitative study (2021)
Journal Article
Knight, H., Carlisle, S., O’connor, M., Briggs, L., Fothergill, L., Al-Oraibi, A., Yildirim, M., Morling, J. R., Corner, J., Ball, J., Denning, C., Vedhara, K., & Blake, H. (2021). Impacts of the COVID-19 pandemic and self-isolation on students and staff in higher education: A qualitative study. International Journal of Environmental Research and Public Health, 18(20), Article 10675. https://doi.org/10.3390/ijerph182010675

This qualitative study explored the impact of COVID-19 self-isolation and social restriction measures on university students, through the perspectives of both students and the staff supporting them. The study comprised 11 focus groups (students) and... Read More about Impacts of the COVID-19 pandemic and self-isolation on students and staff in higher education: A qualitative study.

The use of fluorescence correlation spectroscopy to monitor cell surface β2‐adrenoceptors at low expression levels in human embryonic stem cell‐derived cardiomyocytes and fibroblasts (2021)
Journal Article
Goulding, J., Kondrashov, A., Mistry, S. J., Melarangi, T., Vo, N. T. N., Hoang, D. M., White, C. W., Denning, C., Briddon, S. J., & Hill, S. J. (2021). The use of fluorescence correlation spectroscopy to monitor cell surface β2‐adrenoceptors at low expression levels in human embryonic stem cell‐derived cardiomyocytes and fibroblasts. FASEB Journal, 35(4), Article e21398. https://doi.org/10.1096/fj.202002268r

The importance of cell phenotype in determining the molecular mechanisms underlying β2- adrenoceptor (β2AR) function has been noted previously when comparing responses in primary cells and recombinant model cell lines. Here, we have generated haploty... Read More about The use of fluorescence correlation spectroscopy to monitor cell surface β2‐adrenoceptors at low expression levels in human embryonic stem cell‐derived cardiomyocytes and fibroblasts.

Complex Relationship Between Cardiac Fibroblasts and Cardiomyocytes in Health and Disease (2021)
Journal Article
Hall, C., Gehmlich, K., Denning, C., & Pavlovic, D. (2021). Complex Relationship Between Cardiac Fibroblasts and Cardiomyocytes in Health and Disease. Journal of the American Heart Association, 10(5), Article e019338. https://doi.org/10.1161/jaha.120.019338

Cardiac fibroblasts are the primary cell type responsible for deposition of extracellular matrix in the heart, providing support to the contracting myocardium and contributing to a myriad of physiological signaling processes. Despite the importance o... Read More about Complex Relationship Between Cardiac Fibroblasts and Cardiomyocytes in Health and Disease.

Students’ Views Towards SARS-CoV-2 Mass Asymptomatic Testing, Social Distancing and Self-Isolation in a University Setting during the COVID-19 Pandemic: A Qualitative Study (2021)
Preprint / Working Paper
Blake, H., Knight, H., Jia, R., Corner, J., Morling, J. R., Denning, C., Ball, J. K., Bolton, K., Figeuredo, G., Morris, D., Tighe, P., Villalon, A., Ayling, K., & Vedhara, K. Students’ Views Towards SARS-CoV-2 Mass Asymptomatic Testing, Social Distancing and Self-Isolation in a University Setting during the COVID-19 Pandemic: A Qualitative Study

We aimed to explore university students’ perceptions and experiences of SARS-CoV-2 mass asymptomatic testing, social distancing and self-isolation during the COVID-19 pandemic. This qualitative study comprised of four rapid online focus groups conduc... Read More about Students’ Views Towards SARS-CoV-2 Mass Asymptomatic Testing, Social Distancing and Self-Isolation in a University Setting during the COVID-19 Pandemic: A Qualitative Study.

Discovery of a Novel Polymer for Xeno-Free, Long-Term Culture of Human Pluripotent Stem Cell Expansion (2020)
Journal Article
Nasir, A., Thorpe, J., Burroughs, L., Meurs, J., Pijuan‐Galito, S., Irvine, D. J., Alexander, M. R., & Denning, C. (2020). Discovery of a Novel Polymer for Xeno-Free, Long-Term Culture of Human Pluripotent Stem Cell Expansion. Advanced Healthcare Materials, 10(6), Article 2001448. https://doi.org/10.1002/adhm.202001448

Human pluripotent stem cells (hPSCs) can be expanded and differentiated in vitro into almost any adult tissue cell type, and thus have great potential as a source for cell therapies with biomedical application. In this study, a fully-defined polymer... Read More about Discovery of a Novel Polymer for Xeno-Free, Long-Term Culture of Human Pluripotent Stem Cell Expansion.

Experiences of the COVID-19 pandemic: cross-sectional analysis of risk perceptions and mental health in a student cohort (2020)
Preprint / Working Paper
Jia, R., Knight, H., Blake, H., Corner, J., Denning, C., Ball, J., Bolton, K., Morling, J. R., Coupland, C., Figueredo, G., Morris, D. E., Tighe, P., Villalon, A., Aylling, K., & Vedhara, K. Experiences of the COVID-19 pandemic: cross-sectional analysis of risk perceptions and mental health in a student cohort

Objective: This study examined the COVID-19 risk perceptions and mental health of university students on returning to campus in the midst of the COVID-19 pandemic.

Methods: An online survey was completed during the first four weeks of the academic... Read More about Experiences of the COVID-19 pandemic: cross-sectional analysis of risk perceptions and mental health in a student cohort.

CRISPR/Cas9-mediated generation and analysis of N terminus polymorphic models of β2AR in isogenic hPSC-derived cardiomyocytes (2020)
Journal Article
Kondrashov, A., Mohd Yusof, N. A., Hasan, A., Goulding, J., Kodagoda, T., Hoang, D. M., Vo, N. T., Melarangi, T., Dolatshad, N., Gorelik, J., Hill, S. J., Harding, S. E., & Denning, C. (2021). CRISPR/Cas9-mediated generation and analysis of N terminus polymorphic models of β2AR in isogenic hPSC-derived cardiomyocytes. Molecular Therapy - Methods and Clinical Development, 20, 39-53. https://doi.org/10.1016/j.omtm.2020.10.019

© 2020 During normal- and patho-physiological situations, the behavior of the beta2-adrenoreceptor (β2AR) is influenced by polymorphic variants. The functional impact of such polymorphisms has been suggested from data derived from genetic association... Read More about CRISPR/Cas9-mediated generation and analysis of N terminus polymorphic models of β2AR in isogenic hPSC-derived cardiomyocytes.

Transfection of hPSC-Cardiomyocytes Using Viafect™ Transfection Reagent (2020)
Journal Article
Bodbin, S. E., Denning, C., & Mosqueira, D. (2020). Transfection of hPSC-Cardiomyocytes Using Viafect™ Transfection Reagent. Methods and Protocols, 3(3), Article 57. https://doi.org/10.3390/mps3030057

Twenty years since their first derivation, human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have shown promise in disease modelling research, while their potential for cardiac repair is being investigated. However, low transfection effic... Read More about Transfection of hPSC-Cardiomyocytes Using Viafect™ Transfection Reagent.