Outputs (3)

Enhanced 4-hydroxybutyrate incorporation into the PHA terpolymer of Haloferax mediterranei by heterologous expression of 4-hydroxybutyrate-CoA transferases/synthetases (2024)
Journal Article
Zheng, X., Chacón, M., Allers, T., Dixon, N., & Winterburn, J. (2024). Enhanced 4-hydroxybutyrate incorporation into the PHA terpolymer of Haloferax mediterranei by heterologous expression of 4-hydroxybutyrate-CoA transferases/synthetases. Biochemical Engineering Journal, 212, Article 109498. https://doi.org/10.1016/j.bej.2024.109498

The polyhydroxyalkanoate terpolymer, P[(3HB)-co-(3HV)-co-(4HB)], is a promising plastic alternative for specialized applications, notably in medical and pharmaceutical sectors. Haloferax mediterranei (Hfx), an extreme halophile arch... Read More about Enhanced 4-hydroxybutyrate incorporation into the PHA terpolymer of Haloferax mediterranei by heterologous expression of 4-hydroxybutyrate-CoA transferases/synthetases.

Ion-combination specific effects driving the enzymatic activity of halophilic alcohol dehydrogenase 2 from Haloferax volcanii in aqueous ionic liquid solvent mixtures (2024)
Journal Article
Schindl, A., Hagen, M. L., Cooley, I., Jäger, C. M., Warden, A. C., Zelzer, M., Allers, T., & Croft, A. K. (2024). Ion-combination specific effects driving the enzymatic activity of halophilic alcohol dehydrogenase 2 from Haloferax volcanii in aqueous ionic liquid solvent mixtures. RSC Sustainability, 2(9), 2559-2580. https://doi.org/10.1039/d3su00412k

Biocatalysis in ionic liquids enables novel routes for bioprocessing. Enzymes derived from extremophiles promise greater stability and activity under ionic liquid (IL) influence. Here, we probe the enzyme alcohol dehydrogenase 2 from the halophilic a... Read More about Ion-combination specific effects driving the enzymatic activity of halophilic alcohol dehydrogenase 2 from Haloferax volcanii in aqueous ionic liquid solvent mixtures.

A systematic analysis of affinity tags in the haloarchaeal expression system, Haloferax volcanii for protein purification (2024)
Journal Article
Karan, R., Renn, D., Allers, T., & Rueping, M. (2024). A systematic analysis of affinity tags in the haloarchaeal expression system, Haloferax volcanii for protein purification. Frontiers in Microbiology, 15, Article 1403623. https://doi.org/10.3389/fmicb.2024.1403623

Extremophilic proteins are valuable in various fields, but their expression can be challenging in traditional hosts like Escherichia coli due to misfolding and aggregation. Haloferax volcanii (H. volcanii), a halophilic expression system, offers a so... Read More about A systematic analysis of affinity tags in the haloarchaeal expression system, Haloferax volcanii for protein purification.