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Professor JONATHAN AYLOTT's Outputs (34)

Real-time measurement of the intracellular pH of yeast cells during glucose metabolism using ratiometric fluorescent nanosensors (2017)
Journal Article
Elsutohy, M. M., Chauhan, V. M., Markus, R., Kyyaly, M. A., Tendler, S. J., & Aylott, J. W. (2017). Real-time measurement of the intracellular pH of yeast cells during glucose metabolism using ratiometric fluorescent nanosensors. Nanoscale, 9(18), 5904-5911. https://doi.org/10.1039/c7nr00906b

Intracellular pH is a key parameter that influences many biochemical and metabolic pathways that can also be used as an indirect marker to monitor metabolic and intracellular processes. Herein, we utilise ratiometric fluorescent pH-sensitive nanosens... Read More about Real-time measurement of the intracellular pH of yeast cells during glucose metabolism using ratiometric fluorescent nanosensors.

Electrochemical communication with the inside of cells using micro-patterned vertical carbon nanofibre electrodes (2016)
Journal Article
Rawson, F. J., Cole, M., Aylott, J. W., Milne, W., Collins, C., Jackson, S. K., Silman, N., Mendes, P. M., & Hicks, J. (in press). Electrochemical communication with the inside of cells using micro-patterned vertical carbon nanofibre electrodes. Scientific Reports, 6(37672), https://doi.org/10.1038/srep37672

With the rapidly increasing demands for ultrasensitive biodetection, the design and applications of new nano-scale materials for development of sensors based on optical and electrochemical transducers have attracted substantial interest. In particula... Read More about Electrochemical communication with the inside of cells using micro-patterned vertical carbon nanofibre electrodes.

Quadruple labelled dual oxygen and pH-sensitive ratiometric nanosensors (2016)
Journal Article
Chauhan, V. M., Giuntini, F., & Aylott, J. W. (2016). Quadruple labelled dual oxygen and pH-sensitive ratiometric nanosensors. Sensing and Bio-Sensing Research, 8, https://doi.org/10.1016/j.sbsr.2016.03.007

Nanosensors capable of simultaneously measuring dissolved oxygen concentrations from 0 to 100% saturation and pH over the full physiological range, from pH 3.5 to 7.5, that advance the methods towards understanding of key biological gradients, were s... Read More about Quadruple labelled dual oxygen and pH-sensitive ratiometric nanosensors.

Controlled intracellular generation of reactive oxygen species in human mesenchymal stem cells using porphyrin conjugated nanoparticles (2015)
Journal Article
Lavado, A. S., Chauhan, V. M., Alhaj Zen, A., Giuntini, F., Jones, D. R. E., Boyle, R. W., Beeby, A., Chan, W. C., & Aylott, J. W. (2015). Controlled intracellular generation of reactive oxygen species in human mesenchymal stem cells using porphyrin conjugated nanoparticles. Nanoscale, 7(34), https://doi.org/10.1039/c5nr00795j

Nanoparticles capable of generating controlled amounts of intracellular reactive oxygen species (ROS), that advance the study of oxidative stress and cellular communication, were synthesized by functionalizing polyacrylamide nanoparticles with zinc(I... Read More about Controlled intracellular generation of reactive oxygen species in human mesenchymal stem cells using porphyrin conjugated nanoparticles.

Monitoring the Dissolution Mechanisms of Amorphous Bicalutamide Solid Dispersions via Real-Time Raman Mapping (2015)
Journal Article
Tres, F., Aylott, J., Patient, J. D., Williams, P. M., Treacher, K., Booth, J., Hughes, L. P., Wren, S. A., & Burley, J. C. (2015). Monitoring the Dissolution Mechanisms of Amorphous Bicalutamide Solid Dispersions via Real-Time Raman Mapping. Molecular Pharmaceutics, 12(5), 1512–1522. https://doi.org/10.1021/mp500829v

Real-time in situ Raman mapping has been employed to monitor, during dissolution, the crystallization transitions of amorphous bicalutamide formulated as a molecular dispersion in a copovidone VA64 matrix. The dissolution performance was also investi... Read More about Monitoring the Dissolution Mechanisms of Amorphous Bicalutamide Solid Dispersions via Real-Time Raman Mapping.

An appraisal of the Suzuki cross-coupling reaction for the synthesis of novel fluorescent coumarin derivatives (2014)
Journal Article
Alhaj Zen, A., Aylott, J. W., & Chan, W. C. (2014). An appraisal of the Suzuki cross-coupling reaction for the synthesis of novel fluorescent coumarin derivatives. Tetrahedron Letters, 55(40), https://doi.org/10.1016/j.tetlet.2014.08.058

We report the chemical design and development of 3-aryl-substituted 7-alkoxy-4-methylcoumarins with enhanced fluorogenic properties. The 3-aryl substituents are installed via an optimized Suzuki–Miyaura cross-coupling (SMC) reaction between a 7-alkox... Read More about An appraisal of the Suzuki cross-coupling reaction for the synthesis of novel fluorescent coumarin derivatives.

Human airway smooth muscle maintain in situ cell orientation and phenotype when cultured on aligned electrospun scaffolds (2014)
Journal Article
Morris, G., Bridge, J., Eltboli, O., Lewis, M., Knox, A., Aylott, J. W., Brightling, C., Ghaemmaghami, A., & Rose, F. R. (2014). Human airway smooth muscle maintain in situ cell orientation and phenotype when cultured on aligned electrospun scaffolds. AJP - Lung Cellular and Molecular Physiology, 307(1), Article L38-L47. https://doi.org/10.1152/ajplung.00318.2013

Human airway smooth muscle (HASM) contraction plays a central role in regulating airway resistance in both healthy and asthmatic bronchioles. In vitro studies that investigate the intricate mechanisms that regulate this contractile process are predom... Read More about Human airway smooth muscle maintain in situ cell orientation and phenotype when cultured on aligned electrospun scaffolds.

A novel electrospun biphasic scaffold provides optimal three-dimensional topography forin vitroco-culture of airway epithelial and fibroblast cells (2014)
Journal Article
Morris, G., Bridge, J., Brace, L., Knox, A., Aylott, J. W., Brightling, C., Ghaemmaghami, A. M., & Rose, F. R. (2014). A novel electrospun biphasic scaffold provides optimal three-dimensional topography forin vitroco-culture of airway epithelial and fibroblast cells. Biofabrication, 6(3), Article 035014. https://doi.org/10.1088/1758-5082/6/3/035014

Conventional airway in vitro models focus upon the function of individual structural cells cultured in a two-dimensional monolayer, with limited three-dimensional (3D) models of the bronchial mucosa. Electrospinning offers an attractive method to pro... Read More about A novel electrospun biphasic scaffold provides optimal three-dimensional topography forin vitroco-culture of airway epithelial and fibroblast cells.

Real time Raman imaging to understand dissolution performance of amorphous solid dispersions (2014)
Journal Article
Tres, F., Treacher, K., Booth, J., Hughes, L. P., Wren, S. A., Aylott, J. W., & Burley, J. C. (2014). Real time Raman imaging to understand dissolution performance of amorphous solid dispersions. Journal of Controlled Release, 188, https://doi.org/10.1016/j.jconrel.2014.05.061

We have employed for the first time Raman spectroscopic imaging along with multi-variate curve resolution (MCR) analysis to investigate in real time and in-situ the dissolution mechanisms that underpin amorphous solid dispersions, with data being col... Read More about Real time Raman imaging to understand dissolution performance of amorphous solid dispersions.

Immunocompetent 3D model of human upper airway for disease modeling and in vitro drug evaluation (2014)
Journal Article
Harrington, H., Cato, P., Salazar, F., Wilkinson, M., Knox, A., Haycock, J. W., Rose, F., Aylott, J. W., & Ghaemmaghami, A. M. (2014). Immunocompetent 3D model of human upper airway for disease modeling and in vitro drug evaluation. Molecular Pharmaceutics, 11(7), 2082-2091. https://doi.org/10.1021/mp5000295

The development of more complex in vitro models for the assessment of novel drugs and chemicals is needed because of the limited biological relevance of animal models to humans as well as ethical considerations. Although some human-cell-based assays... Read More about Immunocompetent 3D model of human upper airway for disease modeling and in vitro drug evaluation.

CHOTs optical transducers (2011)
Journal Article
Arca, A., Aylott, J., Marques, L., Clark, M., Somekh, M., Smith, R., Sharples, S., Stratoudaki, T., & Chen, X. (2011). CHOTs optical transducers. Nondestructive Testing and Evaluation, 26(3-4), 353-366. https://doi.org/10.1080/10589759.2011.573555

Laser ultrasonics conventionally use direct absorption in the sample to generate ultrasound and monitor the sample to detect the ultrasound. However, in some circumstances there are significant advantages to using an optical transducer - a device to... Read More about CHOTs optical transducers.