Distinct branches of the N‐end rule pathway modulate the plant immune response

Vicente, Jorge; Mendiondo, Guillermina M.; Pauwels, Jarne; Pastor, Victoria; Izquierdo, Yovanny; Naumann, Christin; Movahedi, Mahsa; Rooney, Daniel J.; Gibbs, Daniel J.; Smart, Katherine; Bachmair, Andreas; Gray, Julie E.; Dissmeyer, Nico; Castresana, Carmen; Ray, Rumiana V.; Gevaert, Kris; Holdsworth, Michael J.

Abstract

© 2018 The Authors. New Phytologist © 2018 New Phytologist Trust The N-end rule pathway is a highly conserved constituent of the ubiquitin proteasome system, yet little is known about its biological roles. Here we explored the role of the N-end rule pathway in the plant immune response. We investigated the genetic influences of components of the pathway and known protein substrates on physiological, biochemical and metabolic responses to pathogen infection. We show that the glutamine (Gln) deamidation and cysteine (Cys) oxidation branches are both components of the plant immune system, through the E3 ligase PROTEOLYSIS (PRT)6. In Arabidopsis thaliana Gln-specific amino-terminal (Nt)-amidase (NTAQ1) controls the expression of specific defence-response genes, activates the synthesis pathway for the phytoalexin camalexin and influences basal resistance to the hemibiotroph pathogen Pseudomonas syringae pv tomato (Pst). The Nt-Cys ETHYLENE RESPONSE FACTOR VII transcription factor substrates enhance pathogen-induced stomatal closure. Transgenic barley with reduced HvPRT6 expression showed enhanced resistance to Ps. japonica and Blumeria graminis f. sp. hordei, indicating a conserved role of the pathway. We propose that that separate branches of the N-end rule pathway act as distinct components of the plant immune response in flowering plants.

Citation

Vicente, J., Mendiondo, G. M., Pauwels, J., Pastor, V., Izquierdo, Y., Naumann, C., Movahedi, M., Rooney, D. J., Gibbs, D. J., Smart, K., Bachmair, A., Gray, J. E., Dissmeyer, N., Castresana, C., Ray, R. V., Gevaert, K., & Holdsworth, M. J. (2019). Distinct branches of the N‐end rule pathway modulate the plant immune response. New Phytologist, 221(2), 988-1000. https://doi.org/10.1111/nph.15387

• Journal Article Type: Article
• Acceptance Date: Jul 11, 2018
• Online Publication Date: Aug 17, 2018
• Publication Date: Jan 1, 2019
• Deposit Date: Jul 12, 2018
• Publicly Available Date: Sep 7, 2018
• Journal: New Phytologist
• Print ISSN: 0028-646X
• Electronic ISSN: 1469-8137
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 221
• Issue: 2
• Pages: 988-1000
• DOI: https://doi.org/10.1111/nph.15387
• Public URL: https://nottingham-repository.worktribe.com/output/945913
• Publisher URL: https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.15387
• Additional Information: Received: 2018-05-18; Accepted: 2018-07-11; Published: 2018-08-17
• Contract Date: Jul 12, 2018