Coordination of meristem and boundary functions by transcription factors in the SHOOT MERISTEMLESS regulatory network

Scofield, Simon; Murison, Alexander; Jones, Angharad; Fozard, John; Aida, Mitsuhiro; Band, Leah R.; Bennett, Malcolm; Murray, James A.H.

Abstract

© 2018. Published by The Company of Biologists Ltd. The Arabidopsis homeodomain transcription factor SHOOT MERISTEMLESS (STM) is crucial for shoot apical meristem (SAM) function, yet the components and structure of the STM gene regulatory network (GRN) are largely unknown. Here, we show that transcriptional regulators are overrepresented among STM-regulated genes and, using these as GRN components in Bayesian network analysis, we infer STM GRN associations and reveal regulatory relationships between STM and factors involved in multiple aspects of SAM function. These include hormone regulation, TCP-mediated control of cell differentiation, AIL/PLT-mediated regulation of pluripotency and phyllotaxis, and specification of meristem-organ boundary zones via CUC1. We demonstrate a direct positive transcriptional feedback loop between STM and CUC1, despite their distinct expression patterns in the meristem and organ boundary, respectively. Our further finding that STM activates expression of the CUC1-targeting microRNA miR164c combined with mathematical modelling provides a potential solution for this apparent contradiction, demonstrating that these proposed regulatory interactions coupled with STM mobility could be sufficient to provide a mechanism for CUC1 localisation at the meristem-organ boundary. Our findings highlight the central role for the STM GRN in coordinating SAM functions.

Citation

Scofield, S., Murison, A., Jones, A., Fozard, J., Aida, M., Band, L. R., …Murray, J. A. (2018). Coordination of meristem and boundary functions by transcription factors in the SHOOT MERISTEMLESS regulatory network. Development, 145(9), Article dev157081. https://doi.org/10.1242/dev.157081

• Journal Article Type: Article
• Acceptance Date: Mar 21, 2018
• Online Publication Date: Apr 12, 2018
• Publication Date: May 1, 2018
• Deposit Date: May 30, 2018
• Publicly Available Date: May 30, 2018
• Journal: Development (Cambridge)
• Print ISSN: 0950-1991
• Electronic ISSN: 1477-9129
• Publisher: Company of Biologists
• Peer Reviewed: Peer Reviewed
• Volume: 145
• Issue: 9
• Article Number: dev157081
• DOI: https://doi.org/10.1242/dev.157081
• Keywords: SHOOT MERISTEMLESS, Gene regulatory network, Arabidopsis, Homeodomain, Meristem, KNOX, Transcriptional regulation
• Public URL: https://nottingham-repository.worktribe.com/output/930113
• Publisher URL: http://dev.biologists.org/content/145/9/dev157081
• Contract Date: May 30, 2018