AUX1-mediated root hair auxin influx governs SCFTIR1/AFB-type Ca2+ signaling

Dindas, Julian; Scherzer, S�nke; Roelfsema, M. Rob G.; von Meyer, Katharina; M�ller, Heike M.; Al-Rasheid, K.A.S.; Palme, Klaus; Dietrich, Petra; Becker, Dirk; Bennett, Malcolm J.; Hedrich, Rainer

Abstract

Auxin is a key regulator of plant growth and development, but the causal relationship between hormone transport and root responses remains unresolved. Here we describe auxin uptake, together with early steps in signaling, in Arabidopsis root hairs. Using intracellular microelectrodes we show membrane depolarization, in response to IAA in a concentration- and pH-dependent manner. This depolarization is strongly impaired in aux1 mutants, indicating that AUX1 is the major transporter for auxin uptake in root hairs. Local intracellular auxin application triggers Ca2+ signals that propagate as long-distance waves between root cells and modulate their auxin responses. AUX1-mediated IAA transport, as well as IAA- triggered calcium signals, are blocked by treatment with the SCFTIR1/AFB - inhibitor auxinole. Further, they are strongly reduced in the tir1afb2afb3 and the cngc14 mutant. Our study reveals that the AUX1 transporter, the SCFTIR1/AFB receptor and the CNGC14 Ca2+ channel, mediate fast auxin signaling in roots.

Citation

Dindas, J., Scherzer, S., Roelfsema, M. R. G., von Meyer, K., Müller, H. M., Al-Rasheid, K., Palme, K., Dietrich, P., Becker, D., Bennett, M. J., & Hedrich, R. (2018). AUX1-mediated root hair auxin influx governs SCFTIR1/AFB-type Ca2+ signaling. Nature Communications, 9(1), Article 1174. https://doi.org/10.1038/s41467-018-03582-5

• Journal Article Type: Article
• Acceptance Date: Feb 26, 2018
• Online Publication Date: Mar 21, 2018
• Publication Date: Dec 1, 2018
• Deposit Date: Jul 17, 2018
• Publicly Available Date: Jul 17, 2018
• Journal: Nature Communications
• Electronic ISSN: 2041-1723
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 9
• Issue: 1
• Article Number: 1174
• DOI: https://doi.org/10.1038/s41467-018-03582-5
• Keywords: General Biochemistry, Genetics and Molecular Biology; General Physics and Astronomy; General Chemistry
• Public URL: https://nottingham-repository.worktribe.com/output/921238
• Publisher URL: https://www.nature.com/articles/s41467-018-03582-5
• Contract Date: Jul 17, 2018