Hydrotropism: analysis of the root response to a moisture gradient

Antoni, Regina; Dietrich, Daniela; Bennett, Malcolm J.; Rodriguez, Pedro L.

Abstract

Hydrotropism is a genuine response of roots to a moisture gradient to avoid drought. An experimental system for the induction of hydrotropic root response in petri dishes was designed by pioneering groups in the field. This system uses split agar plates containing an osmolyte only in a region of the plate in order to generate a water potential gradient. Arabidopsis seedlings are placed on the MS agar plate so that their root tips are near the junction between plain MS medium and the region supplemented with the osmolyte. This elicits a hydrotropic response in Arabidopsis roots that can be measured as the root curvature angle.

Citation

Antoni, R., Dietrich, D., Bennett, M. J., & Rodriguez, P. L. (2016). Hydrotropism: analysis of the root response to a moisture gradient. Methods in Molecular Biology, 1398, 3-9. https://doi.org/10.1007/978-1-4939-3356-3_1

• Journal Article Type: Article
• Acceptance Date: Jan 1, 2016
• Online Publication Date: Feb 12, 2016
• Publication Date: Feb 1, 2016
• Deposit Date: Jul 25, 2016
• Publicly Available Date: Jul 25, 2016
• Journal: Methods in Molecular Biology
• Electronic ISSN: 1940-6029
• Peer Reviewed: Peer Reviewed
• Volume: 1398
• Pages: 3-9
• DOI: https://doi.org/10.1007/978-1-4939-3356-3_1
• Keywords: Hydrotropism, Water potential gradient, Root curvature angle, Moisture gradient, Root growth, Sorbitol, ABA, Arabidopsis
• Public URL: https://nottingham-repository.worktribe.com/output/776393
• Publisher URL: http://link.springer.com/protocol/10.1007%2F978-1-4939-3356-3_1
• Additional Information: The final publication is available at Springer via http://dx.doi.org/10.1007/978-1-4939-3356-3_1
• Contract Date: Jul 25, 2016