Study of NAP adsorption and assembly on the surface of HOPG

Korolkov, Vladimir V.; Allen, Stephanie; Roberts, Clive J.; Gozes, Illana; Tendler, Saul J.B.

doi:10.1016/j.peptides.2014.09.017

Study of NAP adsorption and assembly on the surface of HOPG

Korolkov, Vladimir V.; Allen, Stephanie; Roberts, Clive J.; Gozes, Illana; Tendler, Saul J.B.

Authors

Vladimir V. Korolkov

Stephanie Allen

Professor CLIVE ROBERTS CLIVE.ROBERTS@NOTTINGHAM.AC.UK
HEAD OF SCHOOL - LIFE SCIENCES

Illana Gozes

Saul J.B. Tendler

Abstract

NAP is an octapeptide that has demonstrated a neuroprotective/therapeutic efficacy at very low concentrations in preclinical studies and in a number of clinical trials. Yet little is known about its structural organization at low concentrations. Here, we have employed atomic force microscopy to investigate NAP peptide assembly on graphite in aqueous media at nanomolar concentration. High spatial resolution scans of NAP assemblies reveal their fine structure with clearly resolved single NAP units. This observation leads us to conclude that NAP molecules do not form complex self-assembled structures at nanomolar concentration when adsorbed on graphite surface.

Citation

Korolkov, V. V., Allen, S., Roberts, C. J., Gozes, I., & Tendler, S. J. (2014). Study of NAP adsorption and assembly on the surface of HOPG. Peptides, 62, 55-58. https://doi.org/10.1016/j.peptides.2014.09.017

Journal Article Type	Article
Acceptance Date	Sep 17, 2014
Online Publication Date	Sep 30, 2019
Publication Date	2014-12
Deposit Date	Oct 24, 2014
Publicly Available Date	Dec 31, 2014
Journal	Peptides
Print ISSN	0196-9781
Electronic ISSN	1873-5169
Publisher	Elsevier
Peer Reviewed	Peer Reviewed
Volume	62
Pages	55-58
DOI	https://doi.org/10.1016/j.peptides.2014.09.017
Keywords	NAP; Atomic Force Microscopy; davunetide; adsorption; peptides
Public URL	https://nottingham-repository.worktribe.com/output/735047
Publisher URL	http://www.sciencedirect.com/science/article/pii/S0196978114002861
Additional Information	This article is maintained by: Elsevier; Article Title: Study of NAP adsorption and assembly on the surface of HOPG; Journal Title: Peptides; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.peptides.2014.09.017; Content Type: article; Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.
Contract Date	Oct 24, 2014