Vivien Yeh
The Role of Lipid Chains as Determinants of Membrane Stability in the Presence of Styrene
Yeh, Vivien; Goode, Alice; Johnson, David; Cowieson, Nathan; Bonev, Boyan B.
Authors
Alice Goode
David Johnson
Nathan Cowieson
Professor BOYAN BONEV boyan.bonev@nottingham.ac.uk
PROFESSOR OF BIOPHYSICS
Abstract
Biofermentative production of styrene from renewable carbon sources is crucially dependent on strain tolerance and viability at elevated styrene concentrations. Solvent-driven collapse of bacterial plasma membranes limits yields and is technologically restrictive. Styrene is a hydrophobic solvent that readily partitions into the membrane interior and alters membrane-chain order and packing. We investigate styrene incorporation into model membranes and the role lipid chains play as determinants of membrane stability in the presence of styrene. MD simulations reveal styrene phase separation followed by irreversible segregation into the membrane interior. Solid state NMR shows committed partitioning of styrene into the membrane interior with persistence of the bilayer phase up to 67 mol % styrene. Saturated-chain lipid membranes were able to retain integrity even at 80 mol % styrene, whereas in unsaturated lipid membranes, we observe the onset of a non-bilayer phase of small lipid aggregates in coexistence with styrene-saturated membranes. Shorter-chain saturated lipid membranes were seen to tolerate styrene better, which is consistent with observed chain length reduction in bacteria grown in the presence of small molecule solvents. Unsaturation at mid-chain position appears to reduce the membrane tolerance to styrene and conversion from cis- to trans-chain unsaturation does not alter membrane phase stability but the lipid order in trans-chains is less affected than cis.
Citation
Yeh, V., Goode, A., Johnson, D., Cowieson, N., & Bonev, B. B. (2022). The Role of Lipid Chains as Determinants of Membrane Stability in the Presence of Styrene. Langmuir, 38(4), 1348-1359. https://doi.org/10.1021/acs.langmuir.1c02332
Journal Article Type | Article |
---|---|
Acceptance Date | Dec 17, 2021 |
Online Publication Date | Jan 19, 2022 |
Publication Date | Feb 1, 2022 |
Deposit Date | Jan 6, 2022 |
Publicly Available Date | Jan 19, 2022 |
Journal | Langmuir |
Print ISSN | 0743-7463 |
Electronic ISSN | 1520-5827 |
Publisher | American Chemical Society |
Peer Reviewed | Peer Reviewed |
Volume | 38 |
Issue | 4 |
Pages | 1348-1359 |
DOI | https://doi.org/10.1021/acs.langmuir.1c02332 |
Keywords | Electrochemistry; Spectroscopy; Surfaces and Interfaces; Condensed Matter Physics; General Materials Science |
Public URL | https://nottingham-repository.worktribe.com/output/7167817 |
Publisher URL | https://pubs.acs.org/doi/full/10.1021/acs.langmuir.1c02332 |
Files
acs.langmuir.1c02332
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Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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